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Hongsoonwoonella zoysiae gen. nov., sp. nov., a new member of the family Stappiaceae isolated from a tidal mudflat

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Abstract

A Gram stain-negative bacterial strain, designated SY4-7T, was isolated from rhizosphere mudflat of a halophyte (Zoysia sinica) collected around Seonyu Island, Republic of Korea. Cells of the organism were strictly aerobic, non-sporulating, non-motile rods and grew at 20–42 °C, pH 6–8 and 1–6% (w/v) NaCl. The 16S rRNA gene-based phylogenetic analyses revealed that strain SY4-7T formed an independent cluster separated from the recognized genera of the family Stappiaceae, which was also supported by phylogenomic analysis-based 92-core gene sequences. The type stains of the phylogenetically closest relatives were Stappia indica (95.6% sequence similarity), Stappia stellulata (95.1%) and Roseibium hamelinense (95.1%). The isoprenoid quinone was Q-10. The polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminophospholipids, an unidentified phosphoglycolipid, an unidentified aminolipid, two unidentified phospholipids and an unidentified lipid. The major cellular fatty acids are C18:1ω7c and C19:1 cyclo ω8c. The G + C content of the genomic DNA is 60.7%. Discrimination of the organism from all the recognized genera of the family Stappiaceae was apparent by the chemotaxonomic and phylogenetic features. Based on the results presented here, strain SY4-7T (= KCTC 72226T = NBRC 113902T) represents a novel species of a new genus in the family Stappiaceae, for which the name Hongsoonwoonella zoysiae sp. nov. is proposed.

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Acknowledgements

This research was carried out by the partial support of the Ministry of Food and Drug Safety, Korea (Grant No. MFDS2019-69). The authors would like to thank Dr. S.W. Kown (KACC) and Dr. Y. Muramatsu (NBRC) for providing the type strains of species of the genera Roseibium and Stappia.

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  1. Soon Dong Lee and In Seop Kim contributed equally to this work.

Authors and Affiliations

  1. Institute of Jeju Microbial Resources, BioPS Co., Ltd., Jeju, 63243, Republic of Korea

    Soon Dong Lee

  2. Department of Food Bioengineering, Jeju National University, Jeju, 63243, Republic of Korea

    Sung-Min Kim & Yeong-Sik Byeon

  3. Institute of Hallasan Ecology and Culture, Jeju, 63064, Republic of Korea

    Hong Lim Yang

  4. Department of Biological Sciences and Biotechnology, Hannam University, Daejon, 34054, Republic of Korea

    In Seop Kim

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  1. Soon Dong Lee
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Correspondence to Soon Dong Lee or In Seop Kim.

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Lee, S.D., Kim, SM., Yang, H.L. et al. Hongsoonwoonella zoysiae gen. nov., sp. nov., a new member of the family Stappiaceae isolated from a tidal mudflat. Arch Microbiol 203, 1335–1343 (2021). https://doi.org/10.1007/s00203-020-02083-8

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