Abstract
Elucidation of the acetic acid resistance (AAR) mechanisms is of great significance to the development of industrial microbial species, specifically to the acetic acid bacteria (AAB) in vinegar industry. Currently, the role of population heterogeneity in the AAR of AAB is still unclear. In this study, we investigated the persister formation in AAB and the physiological role of HicAB in Acetobacter pasteurianus Ab3. We found that AAB were able to produce a high level of persister cells (10−2 to 100 in frequency) in the exponential-phase cultures. Initial addition of acetic acid and ethanol reduced the ratio of persister cells in A. pasteurianus by promoting the intracellular ATP level. Further, we demonstrated that HicAB was an important regulator of AAR in A. pasteurianus Ab3. Strains lacking hicAB showed a decreased survival under acetic acid exposure. Deletion of hicAB significantly diminished the acetic acid production, acetification rate, and persister formation in A. pasteurianus Ab3, underscoring the correlation between hicAB, persister formation, and acid stress resistance. By transcriptomic analysis (RNA-seq), we revealed that HicAB contributed to the survival of A. pasteurianus Ab3 under high acid stress by upregulating the expression of genes involved in the acetic acid over-oxidation and transport, 2-methylcitrate cycle, and oxidative phosphorylation. Collectively, the results of this study refresh our current understanding of the AAR mechanisms in A. pasteurianus, which may facilitate the development of novel ways for improving its industrial performance and direct the scaled-up vinegar production.
Key points
• AAB strains form persister cells with different frequencies.
• A. pasteurianus are able to form acid-tolerant persister cells.
• HicAB contributes to the AAR and persister formation in A. pasteurianus Ab3.
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Data availability
The RNA-seq experiment results have been submitted to the Sequence Read Archive (SRA) under accession number PRJNA638183.
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The work was financially supported by grants from the Natural Science Foundation of Zhejiang Province (LY19C200002).
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K.X. conducted the lab work and data analysis, interpreted the data, designed the experiments, and drafted the manuscript. J.X. and C.H. performed part of the experimental work and drew the figures. X.L. conceived the study, acquired the financial support for the project leading to this publication, performed data review, and contributed to the paper writing.
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Xia, K., Han, C., Xu, J. et al. Toxin-antitoxin HicAB regulates the formation of persister cells responsible for the acid stress resistance in Acetobacter pasteurianus. Appl Microbiol Biotechnol 105, 725–739 (2021). https://doi.org/10.1007/s00253-020-11078-w
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DOI: https://doi.org/10.1007/s00253-020-11078-w