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Influence of reductive soil disinfestation or biochar amendment on bacterial communities and their utilization of plant-derived carbon in the rhizosphere of tomato

  • Environmental biotechnology
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Abstract

Root-associated microorganisms play an important role in plant nutrition and productivity. However, our understanding of how a plant-microbiome system responds to pre-planting soil management remains limited. Here, continuous labeling with 13CO2 gas combined with stable isotope probing (SIP) was applied to explore bacterial utilization of plant-derived carbon (C) in the tomato rhizosphere as affected by biochar amendment or reductive soil disinfestation (RSD). Our results showed that RSD treatment strongly shaped the soil bacterial community composition, while biochar soil amendment had little impact on the community in the rhizosphere of tomato. We observed that the bacterial community in the RSD treatment, which actively utilized plant-derived C, belonged to various phyla (i.e., Proteobacteria, Cyanobacteria, Verrucomicrobia, and Acidobacteria), while the genus Streptomyces (phylum Actinobacteria) was the main bacterial taxa that actively utilized plant-derived C in the biochar and control treatments. This study provides evidence that biochar application or RSD pre-planting soil management practices induced distinct bacterial utilization of plant-derived C, which may in turn regulate plant productivity in agricultural systems.

Key points

• Genus Streptomyces was the main bacterial group utilizing plant-derived carbon in both control and biochar treatments.

• Reductive soil disinfestation altered bacterial utilization of plant-derived carbon.

• Biochar did not alter the composition of the bacterial communities but had more labeled bacterial taxa utilizing plant-derived carbon.

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All datasets generated for this study are included in the article/Supplementary Material.

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Funding

This work was supported by the National Natural Science Foundation of China (41525002, 41471206, 41601249, and 41877051), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB15020301), the Talents of Guizhou Science and Technology Cooperation Platform ([2017]5726-52), and the Ningbo Municipal Science and Technology Bureau (2015C10031).

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Authors and Affiliations

  1. Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, People’s Republic of China

    Hongkai Liao, Yaying Li & Huaiying Yao

  2. Zhejiang Key Laboratory of Urban Environmental Processes and Pollution Control, Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, 315800, Ningbo, People’s Republic of China

    Hongkai Liao, Yaying Li & Huaiying Yao

  3. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Hongkai Liao

  4. Guizhou Provincial Key Laboratory of Mountain Environment, Guizhou Normal University, Guiyang, 550001, People’s Republic of China

    Hongkai Liao

  5. Research Center for Environmental Ecology and Engineering, School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan, 430073, People’s Republic of China

    Haoxin Fan & Huaiying Yao

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  1. Hongkai Liao
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  2. Haoxin Fan
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Contributions

HL and HY conceived and designed the research. HL and YL conducted the experiments. HF contributed new reagents or analytical tools. HL and HY wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Huaiying Yao.

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Liao, H., Fan, H., Li, Y. et al. Influence of reductive soil disinfestation or biochar amendment on bacterial communities and their utilization of plant-derived carbon in the rhizosphere of tomato. Appl Microbiol Biotechnol 105, 815–825 (2021). https://doi.org/10.1007/s00253-020-11036-6

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