Can constraint network analysis guide the identification phase of KnowVolution? A case study on improved thermostability of an endo-β-glucanase
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Graphical abstract
Abbreviations
CNA
Constraint Network Analysis
PCR
polymerase chain reaction
HTS
high-throughput screening
EGLII
endoglucanase II
SSM
site-saturation mutagenesis
MTP
96-well microtiter plates
CMC
carboxymethyl cellulose
MD
molecular dynamics
AU
absorbance units
Keywords
KnowVolution
Protein engineering
Constraint network analysis
Thermostability
Cellulase
GH5 endoglucanase
Cited by (0)
- 1
Shared first authors.
- 2
ORCID: 0000-0001-8134-1445.
- 3
ORCID: 0000-0001-5498-0911.
- 4
ORCID: 0000-0003-0089-7156.
- 5
ORCID: 0000-0003-4026-701X.
- 6
ORCID: 0000-0001-8613-1447.
© 2021 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology.