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Development and application of molecular diagnostics and proteomics to bovine respiratory disease (BRD)

Published online by Cambridge University Press:  02 December 2020

John Dustin Loy Open the ORCID record for John Dustin Loy [Opens in a new window]
John Dustin Loy*
Affiliation:
4040 East Campus Loop, 115Q NVDC, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE68583-0906, USA
*
Author for correspondence: John Dustin Loy, 4040 East Campus Loop, 115Q NVDC, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE68583-0906, USA. E-mail: jdloy@unl.edu
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Abstract

Advances in molecular and proteomic technologies and methods have enabled new diagnostic tools for bovine respiratory pathogens that are high-throughput, rapid, and extremely sensitive. Classically, diagnostic testing for these pathogens required culture-based approaches that required days to weeks and highly trained technical staff to conduct. However, new advances such as multiplex hydrolysis probe-based real-time PCR technology have enabled enhanced and rapid detection of bovine respiratory disease (BRD) pathogens in a variety of clinical specimens. These tools provide many advantages and have shown superiority over culture for co-infections/co-detections where multiple pathogens are present. Additionally, the integration of matrix-assisted laser desorption ionization time of flight mass spectrometry (MS) into veterinary diagnostic labs has revolutionized the ability to rapidly identify bacterial pathogens associated with BRD. Recent applications of this technology include the ability to type these opportunistic pathogens to the sub-species level (specifically Mannheimia haemolytica) using MS-based biomarkers, to allow for the identification of bacterial genotypes associated with BRD versus genotypes that are more likely to be commensal in nature.

Keywords

BRD diagnosisMALDI typingMALDI-TOF MSMannheimia haemolyticareal-time PCR bovine respiratory disease
Type
Special issue: Papers from Bovine Respiratory Disease Symposium
Information
Animal Health Research Reviews , Volume 21 , Issue 2 , December 2020 , pp. 164 - 167
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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