Effect of mixed culture of yeast and microalgae on acetyl-CoA carboxylase and Glycerol-3-phosphate acyltransferase expression
Section snippets
Materials
All chemicals used in this study were purchased from Merck (Darmstadt, Germany). The RNA extract kit was obtained from Denazist Asia (Mashhad, Iran). All solutions, prepared with double-distilled water, were kept at 4 °C before further use.
Cultivation of microorganisms and biomass processing
The yeast R. glutinis and the microalgae C. vulgaris used in this study were obtained from the Iranian Biological Resource Center (Tehran, Iran). R. glutinis was maintained on the agar slant of yeast and malt extract (YM) medium. The YM medium contained
Biomass production in pure culture versus co-culture
As C. vulgaris grew slower than R. glutinis, when the mixed culture was prepared from the logarithmic phases of the two species monoculture, the yeast was the prevailing species at the beginning of the co-culture, leading to reduction of the dissolved oxygen in the medium and hence causing anaerobic respiration. Yeast anaerobic respiration produces a large number of volatile organic acids decreasing the environmental pH as the significant factors in fatty acids profile of oleaginous
Acknowledgments
The authors would like to express their gratitude to Ms. Teimorim, the Life Science Engineering Laboratory staff, for her assistance in PCR experiments. The authors declare no conflict of interest.
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