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Generation and Characteristics of Glial Cells from Induced Human Pluripotent Stem Cells

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Abstract—The technology for producing human induced pluripotent stem cells (iPSCs) and the possibility of directed differentiation into specialized cells of all body tissues have opened unique opportunities for studying the molecular genetic basis of the pathogenesis of neurodegenerative diseases in vitro and effective screening for compounds with neuroprotective activity. The aim of this work was to obtain glial cell cultures from iPSCs of a healthy donor and a patient with the familial form of Parkinson’s disease (G2019S mutation in the LRRK2) and to characterize them. At the first stage, we compared the three previously described protocols for the differentiation of glial cells from neural precursors of human iPSCs and selected the method most acceptable under our conditions in terms of the quality and time necessary for obtaining the desired cultures. Glial cell cultures obtained by this method were characterized by the levels of expression of a number of neuroglial differentiation genes. Also, in the resulting cultures, we analyzed the expression of genes of some neurotrophic factors (GDNF, BDNF, NGF, NT3). It was shown that the culture medium conditioned by glial cells from a patient with Parkinson’s disease had a negative effect on the growth of neurites of dopaminergic neurons in differentiated cultures of a healthy donor, decreasing their length by a factor of 2.

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ACKNOWLEDGMENTS

The work was performed using the equipment of the Center for Collective Use of the Institute of Molecular Genetics of the Russian Academy of Sciences “Center for Cell and Gene Technologies.”

Funding

The study was supported by the Russian Foundation for Basic Research, project no. 19-29-04080 mk and by the grant of the program of the Presidium of the Russian Academy of Sciences “Basic Research for Biomedical Technologies.”

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Authors and Affiliations

  1. Institute of Molecular Genetics, National Research Center “Kurchatov Institute”, pl. Akademika Kurchatova 2, 123182, Moscow, Russia

    E. V. Novosadova, E. L. Arsen’eva, S. A. Antonov, E. A. Kazantseva, L. V. Novosadova, O. D. Kurko, V. Z. Tarantul & I. A. Grivennikov

  2. Scientific Center of Neurology, Moscow, Russia

    S. N. Illarioshkin

Authors
  1. E. V. Novosadova
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  2. E. L. Arsen’eva
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  3. S. A. Antonov
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  4. E. A. Kazantseva
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  5. L. V. Novosadova
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  6. O. D. Kurko
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  7. S. N. Illarioshkin
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  8. V. Z. Tarantul
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  9. I. A. Grivennikov
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Corresponding authors

Correspondence to E. V. Novosadova or I. A. Grivennikov.

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Conflict of interest. The authors declare they have no conflict of interest.

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Novosadova, E.V., Arsen’eva, E.L., Antonov, S.A. et al. Generation and Characteristics of Glial Cells from Induced Human Pluripotent Stem Cells. Neurochem. J. 14, 415–423 (2020). https://doi.org/10.1134/S1819712420040066

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  • DOI: https://doi.org/10.1134/S1819712420040066

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