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Bio-synthesis of silver nanoparticles with the brackish water blue-green alga Oscillatoria princeps and antibacterial assessment

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Abstract

This study describes the morphology of a filamentous non-heterocystous blue-green alga (cyanobacterium) Oscillatoria princeps isolated from the Bhabakundalswara sea mouth, its use in biogenic synthesis of silver nanoparticles (AgNPs), along with antimicrobial studies using multidrug resistant (MDR) methicillin-resistant strains of Staphylococcus aureus (MRSA), Streptococcus pyogenes and Escherichia coli, isolated from clinical samples. The synthesized O. princeps -AgNPs were characterized with UV–visible spectrometry, scanning electron microscopy (SEM), X-ray diffraction (XRD), dynamic light scattering analysis (DLS) and Fourier transform infrared spectroscopy (FT-IR) analyses. The synthesized AgNPs had remarkable antibacterial activities against MDR strains of MRSA, S. pyogenes and E. coli with the inhibitory zone sizes, 14–16 mm as recorded from agar well diffusion method, while MIC values were 100, 80, 60 µg/ml, for the respective cited bacterial species. Thus, O. princeps -AgNPs in future would be druggable antibacterials.

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Acknowledgments

This work was funded by the SOADU-PhD fellowship of AK Bishoyi (Regd. No.1981611008/2019). Authors are grateful to School of Pharmaceutical Sciences and Institute of Technical Education and Research (ITER) of the Siksha O Anusandhan Deemed to be University, Bhubaneswar, Odisha for necessary facilities. Authors are thankful to central-instrumental facility center of Orissa University Agriculture and Technology, Bhubaneswar, Odisha; S. N. Bose National Centre for Basic Sciences, Kolkata for DLS and Zeta potential and SRM-DBT Platform for Advanced Life Science Technologies, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, for work with molecular taxonomy.

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  1. Central Research Laboratory, Institute of Medical Sciences and Sum Hospital, Siksha ‘O’ Anusandhan (Deemed To Be University), Kalinga Nagar, Bhubaneswar, Odisha, 751003, India

    Ajit Kumar Bishoyi, Chita Ranjan Sahoo, Arpita Priyadarshinee Sahoo & Rabindra Nath Padhy

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  1. Ajit Kumar Bishoyi
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  2. Chita Ranjan Sahoo
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Bishoyi, A.K., Sahoo, C.R., Sahoo, A.P. et al. Bio-synthesis of silver nanoparticles with the brackish water blue-green alga Oscillatoria princeps and antibacterial assessment. Appl Nanosci 11, 389–398 (2021). https://doi.org/10.1007/s13204-020-01593-7

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