Elsevier

Virus Research

Volume 293, February 2021, 198257
Virus Research

Two viruses from Stylosanthes guianensis may represent a new genus within Potyviridae

https://doi.org/10.1016/j.virusres.2020.198257Get rights and content

Highlights

  • Two potyvirids were found naturally co-infecting stylo plants in Brazil.

  • Named as StyMaV 1 and 2, they represent a new virus genus within the Potyviridae.

  • StyMaV-1 and StyMaV-2 were not efficiently transmitted by mechanical inoculation.

Abstract

Forage crops occupy large areas of tropical pastures for cattle feeding in Brazil. The use of stylos (Stylosanthes spp.) in these pastures, which are leguminous shrubs, has increased in the country due to their outstanding nutritional value and for being an efficient and alternative source for nitrogen fixation in the soil. In recent years, virus-like mosaic symptoms on S.guianensis leaves have often been observed in the field, indicating possible virus-like pathogen infections. In an effort to identify the causal agent, virus semi-purification protocol was performed using symptomatic S. guianensis leaves collected at EMBRAPA Beef Cattle Research Center. Total RNA extracted from this semi-purified preparation was submitted to high-throughput sequencing, which revealed complete genome sequences of novel viruses of the family Potyviridae. These viruses, tentatively named stylo mosaic-associated virus 1 (StyMaV-1) and stylo mosaic-associated virus 2 (StyMaV-2), shared 73 % CP aa identity and 77 % polyprotein aa identity with each other and, after that, being closest related to blackberry virus Y, genus Brambyvirus (only 41 % CP aa identity). Based on ICTV genus demarcation criteria, StyMaV-1 and StyMaV-2 represent new species of a new genus within the family Potyviridae. StyMaV-1 and StyMaV-2 are also not efficiently transmitted to other plant species by mechanical inoculation.

Section snippets

Funding

This research was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Apoio à Pesquisa do Distrito Federal (FAP-DF), and Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA).

CRediT authorship contribution statement

Jamile Mendes de Souza: Methodology, Validation, Data curation, Writing - original draft. Karina Nascimento da Silva Fragoso: Methodology. Anelise Franco Orílio: Validation, Data curation. Fernando Lucas Melo: Conceptualization, Data curation, Writing - review & editing. Tatsuya Nagata: Validation, Writing - review & editing. Celso Dornelas Fernandes: Conceptualization. José Raul Valério: Conceptualization. Fabrícia Zimermann Vilela Torres: Conceptualization. Bruno Barbosa Amaral: Methodology.

Declaration of Competing Interest

The authors declare no conflict of interest.

Acknowledgments

We would like to acknowledge Dr. William Rosa de Oliveira Soares and Dr. Rayane Nunes Lima for providing soybean seeds for experimental inoculation.

References (30)

  • P. Bernardo et al.

    Geometagenomics illuminates the impact of agriculture on the distribution and prevalence of plant viruses at the ecosystem scale

    ISME J.

    (2018)
  • G. Cheng et al.

    Dissecting the molecular mechanism of the subcellular localization and cell-to-cell movement of the sugarcane mosaic virus P3N-PIPO

    Sci. Rep.

    (2017)
  • B.Y.W. Chung et al.

    An overlapping essential gene in the Potyviridae

    Proc. Natl. Acad. Sci. U. S. A.

    (2008)
  • L.A. da Silva et al.

    A new virus found in garlic virus complex is a member of possible novel genus of the family Betaflexiviridae (order Tymovirales)

    PeerJ

    (2019)
  • V.F. Dutra et al.

    Geographic distribution patterns of Leguminosae and their relevance for the Itacolomi State Park, Minas Gerais, Brazil

    Biota Neotrop.

    (2014)
  • Cited by (0)

    View full text