Abstract
The yield potentiality of kharif rice is not completely used even under well-irrigated agro-ecosystem, mainly due to low irradiance by overcast cloud throughout the growing season in eastern India. We observed more than 50% yield reduction compared to the performance of 100 high-yield genotypes for consecutive three years both under open and 30–35% reduced light intensity, mainly by 34%, 25% and 12% reduction of panicle number, grains per panicle and test weight. As per the analysis of variance, genotypic variance explained 39% of the total yield-variation under shade with 58% heritability. Overall, the maintenance of equal panicle per plant in both open and shade has the highest association with shade tolerance. Purnendu, Sashi and Pantdhan19 showed less than 28% yield-reduction by maintenance or even by increasing grain numbers under shade and test weight. On the other hand, maintenance of an equal number of panicle under both situations was the key to the tolerance of Bhasamanik, Sasarang, Rudra and Swarnaprabha. As compared to open, we noticed the improvement of chlorophyll a and b under shade but saw a poor correlation with the shade tolerance index. Comparing the net photosynthesis rate (Pn) in eight genotypes, we found the best tolerant line ranked last with least Pn at low light intensity (<400 µmol m−2 s−1). We also identified diverse parental combinations between newly identified shade tolerant and abiotic stress tolerant high-yielding rice lines following diversity analysis using 54 simple-sequence repeats. Thus, the selected tolerant lines from a large set of genotypes with different adjustment ability to keep up high yield under low light intensity can be used for physiological, molecular analysis as well as pyramiding of traits.
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References
Adhya T. K., Singh O. N. and Ghosh A. 2008 Rice in Eastern India: causes for low productivity and available options. J Rice Res. 2, 1–5.
Barnes J. D., Balaguer L., Manrique E., Elvira S. and Davison A. W. 1992 A reappraisal of the use of DMSO for the extraction and determination of chlorophylls a and b in lichens and higher plants. Environ Exp. Bot. 32, 85–100.
Biswal A. K., Pattanayak G. K., Pandey S. S., Leelavathi S., Reddy V. S. and Tripathy B. C. 2012 Light intensity-dependent modulation of chlorophyll b biosynthesis and photosynthesis by overexpression of chlorophyllide a oxygenase in tobacco. Plant Physiol. 159, 433–449.
Chattopadhyay T., Biswas T., Chatterjee M., Mandal N. and Bhattacharyya S. 2008 Biochemical and SSR marker based characterization of some Bengal landraces of rice suffixed with ‘sail’ in their name. Ind. J. Genet. Plant Breed. 68, 15–20.
Dutta S. S., Tyagi W., Pale G., Pohlong J., Aochen C., Pandey A., Pattanayak A. and Rai M. 2018 Marker–trait association for low-light intensity tolerance in rice genotypes from Eastern India. Mol. Genet. Genomics 293, 1493–1506.
Eberhart S. A. and Russel W. L. 1966 Stability parameters for comparing varieties. Crop Sci. 6, 36–40.
Janardhan K. V., Murty K. S. and Dash N. B. 1980 Effects of low light during ripening period on grain yield and translocation of assimilates in rice varieties. Ind. J. Plant Physiol. 23, 163–168.
Janardhan K. V. and Murty K. S. 1980 Effect of low light during vegetative stage on photosynthesis and growth attributes in rice. Ind. J. Plant Physiol. 23, 156–162.
Jiao D. and Li X. 2001 Cultivar differences in photosynthetic tolerance to photooxidation and shading in Rice (Oryza sativa L.). Photosynthetica 39, 167–175.
Izumi M., Hidema J., Wada S., Kondo E., Kurusu T., Kuchitsu K. et al. 2015 Establishment of monitoring methods for autophagy in rice reveals autophagic recycling of chloroplasts and root plastids during energy limitation. Plant Physiol. 167, 1307–1320.
Kasajima I., Ebana K., Yamamoto T., Takaharac K., Yanob M., Kawai-Yamadaa M. et al. 2011 Molecular distinction in genetic regulation of nonphotochemical quenching in rice. Proc. Natl. Acad. Sci. USA 108, 13835–13840.
Kobata T., Sugawara M. and Takatu S. 2000 Shading during the early grain filling period does not affect potential grain dry matter increase in rice. Agron. J. 92, 411–417.
Liu L., Wang F., Deng Y., Huang D.Y., Liu W. J, Ren W. Y. and Yang W. Y. 2012 Osmotic regulation substance contents and activities of protective enzymes in leaves of different hybrid rice combinations as affected by shading. Chin. J. Rice. Sci. 26, 569–575.
Liu Q., Wu X., Chen B., Ma J. and Gao J. 2014 Effect of low light on agronomic and physiological characteristics of rice including grain yield and quality. Rice Sci. 21, 243−251.
Liu Q. H., Zhou X. B., Yang L. Q., Li T. and Zhang J. J. 2009 Effects of early growth stage shading on rice flag leaf physiological characters and grain growth at grain-filling stage. Chin. J. Appl. Ecol. 20, 2135–2141.
Murchie E. H., Hubbart S., Chen Y., Peng S. and Horton P. 2002 Acclimation of rice photosynthesis to irradiance under field conditions. Plant Physiol. 130, 1999–2010.
Nayak S. K. and Murty K. S. 1980 Effects of varying light intensities on yield and growth parameters in rice. Ind. J. Plant Physiol. 23, 309–316.
Singh S. 2005 Effect of low-light stress at various growth phases on yield and yield components of two rice cultivars. Int. Rice Res. Notes 30, 36–37.
Singh N., Choudhury D. R., Tiwari G., Singh A. K., Kumar S., Srinivasan K. et al. 2016 Genetic diversity trend in Indian rice varieties: an analysis using SSR markers. BMC Genet. 17, 127.
Vanniarajani C., Vinod K. K. and Pereira A. 2012 Molecular evaluation of genetic diversity and association studies in rice Oryza sativa L. J. Genet. 91, 9–19.
Venkateswarlu B. 1996 Influence of low light intensity on growth and productivity of rice (Oryza sativa L.). Plant Soil 47, 713–719.
Voleti S. R. and Singh V. P. 1996 Influence of low light irradiance on grain filling in rice (Oryza sativa L.) cultivars. J. Agron. Crop Sci. 176, 1–4.
Wang L., Deng F., Ren W. J. and Yang W. Y. 2013 Effects of shading on starch pasting characteristics of indica hybrid rice (Oryza sativa L.). PLoS One 8, e68220.
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This research work is supported by ICAR-Incentivizing research scheme. SG and SD acknowledge DST-Jawaharlal Nehru Fellowship and INSPIRE fellowship programme.
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Ganguly, S., Saha, S., Vangaru, S. et al. Identification and analysis of low light tolerant rice genotypes in field conditions and their SSR-based diversity in various abiotic stress tolerant lines. J Genet 99, 24 (2020). https://doi.org/10.1007/s12041-020-01249-z
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DOI: https://doi.org/10.1007/s12041-020-01249-z