Abstract
The content of damaged 8-hydroxy-2-deoxyguanosine nitrogenous bases in the blood DNA of healthy donors and patients with epidermolysis bullosa after exposure to an alternating magnetic field of 550 ± 30 A/m in the frequency range from 3 to 60 Hz in vitro was determined using an enzyme immunoassay. The degree of oxidative DNA damage in epidermolysis bullosa was almost twice as high as in healthy donors. It was shown that there was a significant increase in the level of 8-oxoguanine in the DNA of both groups after the magnetic field treatment, which depended in a complex way on the frequency. The resulting effect is explained by the generation of reactive oxygen species under the influence of a magnetic field and the disruption of DNA repair processes.
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Laboratory diagnostic tests were performed in accordance with the mandatory compliance with the ethical standards set out in the Declaration of Helsinki of 1975 with amendments of 1983.
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Abbreviations: MF, magnetic fields; ROS, reactive oxygen species; 8-ОНdG, 8-hydroxy-2-deoxyguanosine; 8-oxoG, 8‑oxoguanine; EB, epidermolysis bullosa.
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Tekutskaya, E.E., Baryshev, M.G., Gusaruk, L.R. et al. Oxidative Damage to DNA under the Action of an Alternating Magnetic Field. BIOPHYSICS 65, 564–568 (2020). https://doi.org/10.1134/S0006350920040247
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DOI: https://doi.org/10.1134/S0006350920040247