Soluble TatA forms oligomers that interact with membranes: Structure and insertion studies of a versatile protein transporter
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Graphical abstract
Abbreviations
CD
circular dichroism
CN-PAGE
colorless native-polyacrylamide gel electrophoresis
DLS
dynamic light scattering
DOPC
1,2-dioleoyl-sn-glycero-3-phosphocholine
DOPE
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
DSG
disuccinimidyl glutarate
DSS
disuccinimidyl suberate
DTSP
3,3′-dithiobis(succinimidylpropionate)
EM
electron microscopy
FCCS
fluorescence cross-correlation spectroscopy
FCS
fluorescence correlation spectroscopy
GUV
giant unilamellar vesicle
HSQC
heteronuclear single quantum coherence spectroscopy
LUV
large unilamellar vesicle
POPC
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
POPG
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol)
Tat
twin-arginine translocase
Keywords
Twin-arginine translocase
Oligomer
Vesicles
Membrane insertion
Membrane leakage
Electron microscopy
Light scattering
Chemical crosslinking
Circular dichroism
Fluorescence Correlation Spectroscopy
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© 2020 The Author(s). Published by Elsevier B.V.