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Environmentally Sustainable Production of Silver Nanoparticles and Their Effect on Glycine max L. Seedlings

Umweltverträgliche Herstellung von Silbernanopartikeln und ihre Wirkung auf Glycine max L.-Keimlinge

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Abstract

Nanoparticles are regularly released into our atmosphere, and the study of their effect on edible crop plants is of great interest today. This study accounts the enviro-friendly production of silver nanoparticles (SNPs) using Sarcococca saligna (D.Don) Muell.-Arg. leaf extract and the effect of these SNPs on growth and biochemical attributes of soybean (Glycine max L.). The nanoparticles obtained were spherical crystals with a mean size of 52.41 nm. Three soybean varieties namely Rawal‑1, NARC‑2, and Ajmari were exposed to four concentrations of SNPs (0, 25, 50 and 100 ppm) in a completely randomized design. A significant increment in seed germination (Ajmari), root length and shoot length (NARC-2) was recorded at 50 ppm SNPs while the leaf size positively increased at 25 ppm SNPs. The amount of chlorophyll, carotenoids and protein increased at 25 ppm SNPs. All other concentrations caused negative effects on these compounds. SNPs showed a positive effect on growth and biochemical attributes in G. max plant at specific concentrations (50 ppm for growth and 25 ppm for biochemical parameters). Nevertheless, to establish the most appropriate concentration, size and mode of application of SNPs for higher growth and maximum yield, a comprehensive experimentation is required.

Zusammenfassung

Nanopartikel werden regelmäßig in unsere Atmosphäre freigesetzt und die Untersuchung ihrer Wirkung auf essbare Nutzpflanzen ist heute von großem Interesse. Diese Studie beschreibt die umweltfreundliche Produktion von Silbernanopartikeln (SNPs) unter Verwendung eines Sarcococca saligna (D.Don) Muell.-Arg.-Blattextrakts und die Wirkung dieser SNPs auf das Wachstum und die biochemischen Eigenschaften von Sojabohnen (Glycine max L.). Die erhaltenen Nanopartikel waren kugelförmige Kristalle mit einer mittleren Größe von 52,41 nm. Drei Sojabohnensorten, nämlich Rawal‑1, NARC‑2 und Ajmari, wurden in einem vollständig randomisierten Design vier Konzentrationen von SNPs (0, 25, 50 und 100 ppm) ausgesetzt. Ein signifikanter Anstieg der Samenkeimung (Ajmari), der Wurzellänge und der Sprosslänge (NARC-2) wurde bei 50 ppm SNPs festgestellt, während die Blattgröße bei 25 ppm SNPs zunahm. Die Menge an Chlorophyll, Carotinoiden und Protein stieg bei 25 ppm SNPs an. Alle anderen Konzentrationen hatten negative Auswirkungen auf diese Komponenten. Die SNPs zeigten einen positiven Effekt auf das Wachstum und die biochemischen Eigenschaften in G. max-Pflanzen bei bestimmten Konzentrationen (50 ppm für Wachstum und 25 ppm für biochemische Parameter). Um die am besten geeignete Konzentration, Größe und Art der Anwendung von SNPs für ein höheres Wachstum und einen maximalen Ertrag zu ermitteln, sind jedoch umfassende Experimente erforderlich.

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Acknowledgements

We are thankful to IST Islamabad for providing scanning electron microscopy and x‑ray diffraction analysis and QAU Islamabad for UV-vis spectroscopy.

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Authors and Affiliations

  1. Department of Botany, University of the Poonch Rawalakot, 12350, Azad Kashmir, Pakistan

    Hina Sharif, Ansar Mehmood, Aneela Ulfat & Khawaja Shafique Ahmad

  2. Department of Food Science and Technology, University of Poonch Rawalakot (UPR), 12350, Azad Kashmir, Pakistan

    Imtiaz Hussain

  3. Department of Botany, University of Azad Jammu and Kashmir (UAJK), 13100, Muzaffarabad, Pakistan

    Rizwan Taj Khan

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  1. Hina Sharif
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  2. Ansar Mehmood
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  3. Aneela Ulfat
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  5. Imtiaz Hussain
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Correspondence to Ansar Mehmood.

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H. Sharif, A. Mehmood, A. Ulfat, K.S. Ahmad, I. Hussain and R.T. Khan declare that they have no competing interests.

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Sharif, H., Mehmood, A., Ulfat, A. et al. Environmentally Sustainable Production of Silver Nanoparticles and Their Effect on Glycine max L. Seedlings. Gesunde Pflanzen 73, 95–103 (2021). https://doi.org/10.1007/s10343-020-00532-4

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  • DOI: https://doi.org/10.1007/s10343-020-00532-4

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