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Differentially expressed proteins in the development of protocorm-like bodies of Cattleya tigrina A. Richard

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Abstract

Cattleya tigrina is an endangered Brazilian native orchid with ornamental potential. Biotechnological tools based on tissue culture techniques are effective for its mass propagation and conservation. Protocorm-like bodies (PLBs) were induced from leaves in culture medium supplemented with plant growth regulators, and generated complete in vitro plants. To deepen on the mechanism underlying this morphogenetic route, this study is aimed to identify and characterize differentially expressed proteins during C. tigrina PLBs development. Leaves were inoculated in in culture medium Murashige and Skoog (MS) supplemented with 9 μM Thidiazuron, and PLBs t were collected after 30, 60 and 100 days in culture. Proteomic analysis performed by two-dimensional electrophoresis and MALDI-TOF mass spectrometry detected 122, 132 and 447 protein after 30, 60 and 100 days, respectively. During PLBs development, 36 proteins were differentially expressed. Proteins related to energy and carbohydrate metabolism, cell proliferation, protein processing, and secondary metabolism and phytohormones were assessed showing their relevance in the process of development of PLBs. Many proteins related to metabolic and energy processes were identified after 60 and 100 days in culture, suggesting their role in which is cell division and differentiation. Enolase was only present after 100 days in culture, thus being a candidate as a molecular marker in this developmental phase.

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Acknowledgements

The authors thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES-Brasília/Brazil, Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq—Brasília/Brazil and Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina—FAPESC—Florianópolis/Brazil (Proc. 2780/2012-4) for fellowship research grants, and financial support. The authors also thank Centro de Biologia Molecular Estrutural da Universidade Federal de Santa Catarina (CEBIME-UFSC) where part of the work was developed. The information present in this work is part of the PhD Thesis of the author Daniela De Conti.

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Authors and Affiliations

  1. Faculdade do Vale do Araranguá, Av. Getúlio Vargas, 415-Centro, Araranguá, Santa Catarina, 88900-000, Brazil

    Daniela De Conti

  2. Departament of Biology and Chemistry, Universidade de Sucre, Barrio Puerta Roja, 28 # 5-267, Ciudad Sincelejo, Estado Sucre, Colombia

    Jenny Paola Corredor-Prado

  3. Graduate Program in Plant Genetic Resources, Federal University of Santa Catarina, Admar Gonzaga, Road, 1346, Itacorubi, Florianópolis, Santa Catarina, 88034-001, Brazil

    Jessica Cavalheiro Ferreira Bueno, Gabriela Claudia Cangahuala-Inocente, Miguel Pedro Guerra & Rosete Pescador

  4. Graduate Program in Agricultural and Natural Ecosystems, Federal University of Santa Catarina, Curitibanos Campus, Ulysses Gaboardi Road, Km 3, Curitibanos, Santa Catarina, 89520‑000, Brazil

    Miguel Pedro Guerra

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  1. Daniela De Conti
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Correspondence to Daniela De Conti.

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De Conti, D., Corredor-Prado, J.P., Bueno, J.C.F. et al. Differentially expressed proteins in the development of protocorm-like bodies of Cattleya tigrina A. Richard. Acta Physiol Plant 42, 181 (2020). https://doi.org/10.1007/s11738-020-03170-5

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