Analyzing Branch‐specific Dendritic Spikes Using an Ultrafast Laser Scalpel

Introduction

Dendrites perform computation with the aid of dendritic spikes (D-spikes) [1–3]. A D-spike is characterized by a nonlinear intensification of the membrane potential caused by regenerative activation of voltage-gated ion-channels. The broad temporal profiles of D-spikes (>20 ms) enable neurons to boost their firing probability with coincident synaptic inputs occurring within a broad time window [4, 5]. Hence, coincidence detection with the aid of D-spikes presents an important computing mechanism in pyramidal neurons [6, 7]. In vitro studies have shown that D-spikes occur in cortical pyramidal neurons [8–10] as well as hippocampal neurons [11–14] and their role in single neuron computation impacts information processing in neuronal circuits collectively.

D-spikes have been observed in several locations in the dendritic tree of cortical pyramidal neurons. In layer five pyramidal neurons (L5PNs), D-spikes mediated by voltage-gated calcium channels (VGCCs) have been reported to occur at the nexus of the apical tuft dendrites, which causes a global depolarization of the main apical trunk and apical branches [10, 15, 16]. Moreover, N-Methyl-D-Aspartate (NMDA) spikes have been reported to occur at the apical tuft [17] and basal dendrites [18, 19] of pyramidal neurons.

We have recently reported that D-spikes also occur in apical oblique dendrites of L5PNs [20, 21]. D-spikes in apical oblique branches are triggered by a low-frequency (f ≥ 60 Hz) train of action potentials (APs), which we initially investigated using a multi-compartment model of a L5PN and confirmed experimentally via functional calcium imaging [21]. As APs back-propagate to the dendritic tree, they activate VGCCs at the dendrite to generate a spike. We have numerically characterized the D-spike in oblique branches as a fast sodium spike followed by a broad depolarization due to regenerative activation high-voltage activated calcium channels [20].

D-spikes can be investigated by pharmacologically blocking certain channels along specific regions of the dendritic tree [17,22,23]. However, targeted and highly-localized drug delivery is challenging to perform especially on thin and complex dendritic morphologies such as the apical oblique branches of L5PNs. Neighboring dendrites could be unnecessarily applied with drugs and therefore not viable for analyzing D-spikes that occur only in a few among many intertwined dendrites.

An alternative way to investigate D-spikes is by dendrotomy. Bekkers and Häusser [24] used a pair of micropipettes to pinch the main apical trunk and to study its active and passive properties. Although their work did not focus on D-spikes evoked in particular dendritic domains, their findings show that dendrotomy of the apical trunk of L5PNs reduced the overall excitability and the afterdepolarization (ADP) at the soma. While the ADP comes from multiple sources across the dendritic tree [25], it has been primarily associated with the recruitment of D-spikes in specific regions such as the nexus of the apical tuft [15,16] and oblique branches of L5PNs [21]. However, unlike the wide reach of a D-spike at the nexus that extends throughout the tuft, investigating spikes in select oblique branches requires identifying the specificity of spike generation. Which among the oblique dendrites are most likely to produce a spike? To answer this question, we need a tool that allows us to tease out the influence of certain branches to the overall neuronal function.

Here, we aim to use dendrotomy to investigate branch-specific D-spikes in apical oblique dendrites of L5PNs. To aid our analysis, we numerically simulated the process and implemented dendrotomy in vitro using L5PNs in acute brain slices. While the technique used by Bekkers and Häusser [24] has been effective for dendrotomy of thick dendrites (e.g., apical trunk), using micropipettes to pinch thin obliques without affecting its neighboring dendrites can be very challenging. Hence, we adopted a highly-targeted optical ablation approach using a tightly-focused ultrafast laser scalpel [26]. We evoked D-spikes in apical oblique dendrites [21] and observed the changes in the ADP profile following dendrotomy. Since dendrotomy via an ultrafast laser scalpel is an irreversible process, it would take a large number of experiments to draw neuro-physiological conclusions as to the extent of specificity of spike generation among oblique branches. Nonetheless, our results provide the groundwork for using a laser scalpel to tease out the computing mechanisms of certain branches among complex dendritic arborizations in cortical pyramidal neurons.

Multi-Compartmental Model

We implemented our numerical experiments via NEURON and Python [27] using a multi-compartmental model of an L5PN shown in Figure 1A [28]. The procedure for numerical experiments is described in the Supplementary Methods 1. We slightly modified the L5PN model to shape the neuron’s response closer to our empirical data [21]. Our experiments show that oblique branches of L5PNs are less excitable compared to the model. Hence, we incorporated potassium (K⁺) ion-channels (fast-activating (K_T) and persistent-activating (K_PST) K⁺ channels) to regulate dendritic excitability and control the extent of backpropagating APs, as previously implemented in models [29–31], and experiments involving L5PNs [31,32] and hippocampal neurons [13,22]. Detailed description of the modified model is described in Supplementary Methods 2.

FIGURE 1. Dendritic spikes generated from the L5PN multi‐compartment should be in one line (move the compartment to the above line) model. (A) The morphology of the L5PN used in the model. The membrane potential at (B) the nexus of the apical tuft and (C) the soma during a four-AP train with frequency f = 100 Hz. The membrane potential at the oblique dendrites (D) O#3; and (E) O#1, and at the (F) soma during a two-AP train with f = 70 Hz.

To generate D-spikes, we injected brief current pulses (Amplitude 3–4 nA, Pulse-width 2 ms) at the soma to elicit a train of APs. The frequency of the train of two or four APs ranges from 20 ≤ f ≤ 120 Hz. When the APs back-propagate to the dendrites, they can initiate D-spikes at certain areas of the dendritic tree. For example, a train of four action potentials (four-AP train) with f = 100 Hz can initiate a D-spike at the nexus of the apical tuft dendrites [15]. Figure 1B shows the trace of the membrane potential, V_m, at the nexus of the apical tuft during a four-AP train while Figure 1C shows the V_m at the soma. On the other hand, a two-AP train elicits a D-spike at certain oblique branches as shown in Figure 1D and corresponding somatic V_m recording shown in Figure 1F. When a D-spike is generated, the broad temporal depolarization of the spike manifests at the soma as an ADP that follows after the last AP of the train. Somatic V_m recordings in Figures 1C,F show traces with a slight bump following the last AP. While there are multiple sources of the ADP, a larger ADP is observed when the AP train evokes a D-spike in one or multiple dendrites. Note that not all oblique branches evoke a D-spike during a two-AP train. Other dendrites, such as the proximal oblique O#1, do not evoke a D-spike as observed by the absence of a temporally broad depolarization (Figure 1E). In this particular L5PN model, branch-specific D-spikes are evoked only at two oblique dendrites: O#3 (Figure 1D) and O#5 (data not shown) [21].

The minimum frequency of the AP train required to generate the D-spike is referred to as the critical frequency, f_c [15]. The f_c is derived by plotting the neuron’s response (dendritic or somatic ADP recording) as a function of frequency. The frequency response is fitted with a Sigmoid function and f_c is the inflection point where the step increase in the neuron’s response occurs. Electrophysiological recording at the dendrite where the spike occurs provides for an accurate characterization of D-spikes but could be challenging especially when the dendrite is thinner than the tip of the electrode. Optical recording via calcium or voltage indicators can also be used to record dendritic activity [33]. However, both optical and electrophysiological approaches to record dendritic activity become ineffective after dendrites are severed from the neuron. Hence, for this work, we use electrophysiological recording at the soma to characterize D-spikes based on the changes in the ADP profile before and after dendrotomy. Note, however, that the ADP is a net depolarization from multiple sources in the neuron’s dendritic tree [25] and is therefore ambiguous to use it solely to identify branches where D-spikes occur. Nonetheless, we have prior work that shows calcium responses recorded from oblique branches showing D-spikes from these dendrites are correlated with ADP profiles at the soma [21].

We measured the ADP from the somatic recordings to extract the critical frequencies of the dendritic spikes. Figures 2A,B are voltage traces taken from the soma of the L5PN model during two-AP and four-AP trains, respectively, with the traces aligned to the peak of the last AP (t = 0). The average ADP is calculated as $\langle ADP\rangle = {\left(\Delta t\right)}^{-1}\underset{t_o}{\overset{t_o+ \Delta t}{{\displaystyle \int }}}{V}_m dt$, where Δt = 5 ms is the time-window used for averaging the ADP from t_o= 10 ms (for two-AP train) and t_o = 20 ms (for four-AP train). Figure 2C shows frequency responses of the $\langle ADP\rangle$. The plots are fitted with a Sigmoid function ($S\left(f\right)=A/\left(1+\text{exp}[-\beta \left(f-f_{ c}\right)]\right)$, to obtain critical frequencies, f_c = 50 Hz and f_c = 90 Hz, following two-AP and four-AP train stimulation, respectively. The fitting parameters, A (mV) and β (Hz⁻¹), refer to the amplitude and slope of the Sigmoid function, which can be used to evaluate the non linearity of the frequency response.

FIGURE 2. After depolarization associated with D-spikes. The somatic membrane potential aligned to the last AP, following (A) two-AP, and (B) four-AP trains with frequencies from 20 ≤ f ≤ 120 Hz. (C) The frequency response of the average ADP, 〈$ADP$〉, taken over Δt during two-AP (blue squares) and four-AP trains (red circles).

Oblique Branch Spike in L5PNs in vitro

We prepared 300-μm-thick brain slices from Wistar male rats (P26–P34 days old). Prior to slicing, the rats were sedated by inhalation of 2–4% isoflurane with oxygen (with flow of 3 L/min) and decapitated according to the protocol approved by the Animal Experimentation Ethics Committee of the Australian National University (Ethics Protocol ID: A2018/35). Somatosensory cortical brain slices were prepared using a vibratome and perfused with oxygenated (95% O₂/5% CO₂) extracellular solution containing (in mM): 125 NaCl, 25 NaHCO₃, 25 glucose, 3 KCl, 1.25 NaH₂PO₄, 2 CaCl₂, 1 MgCl₂, pH 7.4. Whole-cell patch-clamp recordings were made with 4–6 MΩ pipettes filled with an intracellular solution containing (in mM): 115 K-gluconate, 20 KCl, 10 HEPES, 10 phosphocreatine, 4.0 ATP-Mg, 0.3 GTP, and 0.13 Alexa Fluor 488 dye (Sigma Aldrich) [33].

For patch-clamp recordings and rendering of the neuron’s dendritic tree, the tissue sample was transferred to our custom-built two-photon (2P) microscope shown in Figure 3A [26, 34]. The microscope uses a near-infrared femtosecond laser (Chameleon, Coherent Scientific) and galvanometer scanning mirrors to render a three-dimensional (3D) image of the patched neuron. The microscope is built from a standard differential interference contrast microscope (Olympus BX50) and modified to incorporate a two-photon and holographic projection function [34]. While the microscope has other modalities (e.g., holographic projection), this work only used the mechanical two-dimensional (2D) beam scanners for imaging and positioning the laser focus onto a dendrite. Figure 3B shows a representative image of a L5PN loaded with Alexa Fluor 488.

FIGURE 3. Experimental setup and electrophysiological recordings: (A) Schematic of our custom-built two-photon microscope used for laser dendrotomy. Different modalities are merged using a polarizing beam splitter (PBS), while the emitted fluorescence is acquired via a photomultiplier tube (PMT). (B) A flattened image stack of an L5PN intracellularly loaded with Alexa Fluor 488 dye. (C) The membrane potential recorded from the soma following a two-AP train with frequencies from 40 to 150 Hz aligned to the second AP (D) The frequency response of the $ADP$ from the recordings in (C). Scale bar in (B) is 50 μm.

After mapping the 3D morphology of the entire neuron, we used the same protocol as our model to determine the critical frequencies from the changes in the ADP profile. We injected two pulse trains (Amplitude of 3–4 nA, Pulse-width of 2 ms) to produce a two-AP train with frequencies ranging from 4 < f < 150 Hz and the somatic recording is shown in Figure 3C. We took the $\langle ADP\rangle$ over Δt and the frequency response of the $\langle ADP\rangle$ shows a non linear step increase at the critical frequency (Figure 3D). We fitted a Sigmoid function on the frequency response of the $\langle ADP\rangle$ and identified L5PNs that exhibited D-spikes.

Dendrotomy of Oblique Branches

Model

To perform dendrotomy in silico, dendritic segments were disconnected from the model. We analyzed the temporal profiles of the membrane potentials at the soma during two-AP trains and investigated the changes in the ADP before and after cutting certain oblique branches. If the increase in the ADP is associated with the generation of a D-spike in a particular oblique branch, we hypothesize that removing that branch alters the ADP profile. On the other hand, removal of an oblique branch that do not support a D-spike, maintains the ADP profile as well as the frequency response of the $\langle ADP\rangle$.

Our numerical experiments show that dendrotomy of certain oblique branches variably affected the ADP and confirming our hypothesis. Cutting a branch that evokes a D-spike (e.g., O#3, Figure 4A) altered the ADP profile. Figure 4B shows traces of the somatic V_m at various frequencies of the two-AP train (aligned with the second AP) for an intact dendritic tree. The frequency response of the $\langle ADP\rangle$ is fitted with a sigmoid function and shows a non linear step increase occurring at f_c = 50 Hz (Figure 4C). The fitting parameters of the sigmoid function resulted in an amplitude, A = 13 ± 1 mV and slope, β = 0.22 ± 0.09 Hz⁻¹. Cutting of O#3 altered the ADP profile (Figure 4D) and consequently the frequency response of the $\langle ADP\rangle$ (Figure 4E). The increase in the $\langle ADP\rangle$ at frequencies less than 40 Hz is potentially due its extended branch morphology, which sets the branch to act as strong sink during back-propagation of an AP. The removal of O#3 potentially increased the efficacy of AP invasion to other apical dendrites resulting to a larger somatic depolarization. At frequencies greater than 40 Hz, the $\langle ADP\rangle$ follows a trend that can be fitted with a sigmoid function with A = 8 ± 1 mV and β = 0.10 ± 0.03 Hz⁻¹. The changes in the fitting parameters before (Pre) and after (Post) dendrotomy of O#3 are quantified in Figure 4F, which shows a significant decrease in A and β. The error bars represent the uncertainty of the fit.

FIGURE 4. Numerical dendrotomy of oblique branches: (A) A magnified view of the L5PN model following dendrotomy of oblique branches O#3 or O#1 (red). (B) Somatic traces during a two-AP train, and (C) corresponding frequency response of the $ADP$ before dendrotomy. (D) Somatic traces after cutting O#3 and (E) corresponding frequency response of the $\langle ADP\rangle$. (F) Quantification of the changes in fitting parameters, amplitude (A) and slope (β), of the Sigmoid function before (Pre) and after (Post) dendrotomy. Cutting oblique branch O#1 and corresponding (G) somatic traces during a two-AP train, and (H) the frequency response of the $\langle ADP\rangle$(I) Changes in A and β after cutting O#1. The error bars show uncertainty of the Sigmoid fit.

On the other hand, cutting branch O#1 (see Figure 4A) did not change the ADP profile (compare Figures 4G,B) as well as the frequency response of the $\langle ADP\rangle$ (compare Figures 4H,C). Note that O#1 did not display a D-spike even at higher frequencies of the two-AP train (Figure 1E). Likewise, the changes in the fitting parameters before and after dendrotomy of O#1 are quantified in Figure 4I, which shows a slight increase in the amplitude (A = 14 ± 1 mV) while the slope is slightly reduced (β = 0.16 ± 0.04 Hz⁻¹). Differences in neuronal responses when cutting O#1 and O#3 confirm branch-specific properties of certain oblique dendrites.

Experiment

We performed targeted dendrotomy using our-custom built 2P microscope (Figure 3A) by positioning the laser focus onto specific dendrites via the 2D beam scanners. The transverse resolution of the incision was within the diffraction limit (diameter ∼1 μm) matching that of typical diameters of apical oblique branches (Figure 5A). Note that a slight offset of the focus with respect to the dendrite reduced the efficacy of the cut. The average power of the laser used for dendrotomy is $P$ = 80 mW with an estimated irradiance of 129 mW/μm² (for 1.0NA Objective), 0.5–3.0 s exposure time and λ = 890 nm. The average power was measured using an optical power meter (Thorlabs) with a photodiode head (S132C, Thorlabs) positioned at the front aperture of the objective lens. We confirmed successful dendrotomy by imaging the targeted dendrite (Figure 5B).

FIGURE 5. Laser dendrotomy of apical oblique dendrites. Fluorescence image of an oblique branch (A) before dendrotomy, and (B) after dendrotomy, (C) the current recording at the soma. After dendrotomy, the current increased sharply indicating AP firing of the neuron. The current then decayed to a new value after 10 min as membrane at the cut site partially sealed, (D) the holding current before (Pre) and after (Post) dendrotomy required to maintain the membrane potential at −65 mV. Scale bars in (A) and (B) are 10 µm.

The L5PNs were held in voltage clamp at their typical resting membrane potential of −65 mV. After dendrotomy, the L5PNs depolarized and fired APs, as indicated by a large negative current in the voltage-clamp recording (Figure 5C). The L5PNs were left undisturbed for 10 min until the holding current, I_h, settled to a value that maintained a resting membrane potential of −65 mV (Figure 5D). The new holding current suggests that the membrane at the cut site has not fully sealed and partial resealing of the cut site caused the neuron to be slightly depolarized after dendrotomy. Despite the change in the holding current, the neurons were still able to fire action potentials when current pulses were injected onto the soma.

We analyzed the ADP of L5PNs before and after dendrotomy. Figure 6 shows somatic recordings before (column A) and after (column B) dendrotomy as well as the frequency response of the $\langle ADP\rangle$ (column C) from four neurons (rows 1–4). The L5PNs exhibited critical frequencies, f_c = 76–100 Hz during two-AP trains. Dendrotomy of certain oblique branches altered the frequency response of the $\langle ADP\rangle$, which confirmed our hypothesis. Across all neurons, the $\langle ADP\rangle$ decreased by 2–5 mV, indicative of a decrease in dendritic excitability (compare columns A and B in Figure 6). Column C shows two neurons (#1 and #2) with altered frequency response of the $\langle ADP\rangle$. We quantified the changes in the frequency response by comparing the fitting parameters of the Sigmoid function before (Pre) and after (Post) dendrotomy (column D). Prior to cutting, the non linear step increase in the $\langle ADP\rangle$ at their respective critical frequencies yielded amplitudes, A = 5.8 ± 0.3, 4.3 ± 0.15, 2.8 ± 0.2 and 7.3 ± 0.4 mV and slope, β = 3 ± 1, 0.15 ± 0.02, 0.3 ± 0.1 and 0.15 ± 0.03 Hz⁻¹ from neurons (rows) #1 to #4, respectively. However, cutting an oblique dendrite of two L5PNs reduced the amplitude A = 0.53 ± 0.08 mV and 1.7 ± 0.2 mV for neurons #1 and #2, respectively. The change in the slope for neuron #1 (β = 0.16 ± 0.09 Hz⁻¹) is significant but not much for neuron #2 (β = 0.1 ± 0.04 Hz⁻¹). Nonetheless, these two neurons resembled to a flattened frequency response after dendrotomy. Such response is representative of cutting O#3 in the model (Figure 4).

FIGURE 6. ADP profile and frequency responses before and after dendrotomy. Recordings from four L5PNs (rows 1–4) showing: the somatic membrane potential traces before (column A) and after (column B) dendrotomy aligned to the last AP; (column C) the corresponding frequency response of the $ADP$; and (column D) the changes in the fitting parameters of the Sigmoid function, amplitude (A) and slope (β), before (pre) and after (post) dendrotomy. The error bars show uncertainty of the Sigmoid fit.

On the other hand, two L5PNs showed minimal changes in the ADP profile (neurons #3 and #4 Figure 6) after dendrotomy. From column D (Post), cutting the proximal oblique dendrites resulted in a slight increase in the amplitude, A = 3.4 ± 0.2 mV and 11.6 ± 0.4 mV for neurons #3 and #4, respectively. No significant changes in their slopes were observed with β = 0.21 ± 0.05 (neuron #3) and 0.14 ± 0.02 Hz⁻¹ (neuron #4). Such response is representative of cutting the proximal O#1 branch in the model (Figure 4).

These experimental results agree with our model and suggest that removal of a single dendrite, which potentially evoked a D-spike, affected the overall excitability of L5PNs and altered the frequency response of the $\langle ADP\rangle$. On the other hand, cutting a dendrite that does not support a D-spike, maintained the ADP profile and frequency response of the $\langle ADP\rangle$.

Discussion

We investigated the influence of branch-specific D-spikes to neuronal function by pruning certain oblique branches of cortical L5PNs. Using a multi-compartment model of a L5PN, we removed segments of the oblique branches and observed the changes in neuronal output via the frequency response of the $\langle ADP\rangle$. We then confirmed the results of the model by using an ultrafast laser scalpel for cutting apical oblique branches in vitro. In the model, cutting branches that exhibited D-spikes resulted in the reduction of the $\langle ADP\rangle$ at frequencies above fc. Experimentally, we observed neurons that exhibited flattening of the $\langle ADP\rangle$ frequency response after laser dendrotomy of an oblique branch. Moreover, we also observed neurons that showed no change in the ADP profile after laser dendrotomy and they represent conditions in our model where we cut a branch that did not exhibit a D-spike.

Dendrotomy and Limitations

Dendritic branching strongly influences AP-firing [35], AP back-propagation [36], and the coupling of somatic-AP spike and Ca²⁺-AP spike zone at the nexus [5]. The ability to manipulate the neuron’s dendritic morphology with the use of micropipettes [24, 37] or laser [26, 38, 39], is important to understand the relation between structure and function. Laser dendrotomy offers a flexible approach since the focus of the laser can be positioned to target different branches of the neuron. We were able to adapt the set of laser parameters reported previously by Go et al. [26] to prune thin oblique branches while minimizing optically-induced damage on the L5PNs.

The main drawback with our approach is the fact that dendrotomy is an irreversible process. As such, using the technique will require data from several neurons in order to draw statistically significant conclusions on the function of the dendrites as well as their impact on the physiology of neurons as a whole. Moreover, the removal of too much dendritic segments may affect the overall excitability of the neuron. Oblique branches act as a sink for currents propagating along the main apical trunk. The number of oblique branches and their distribution along the trunk determine how effective APs back-propagate up to the nexus of the apical tuft dendrites [5].

Branch-Specific Spikes

Branch-specific D-spikes in apical oblique branches are recent addition to the well-documented spikes in other regions of the L5PN’s dendritic tree [15, 17, 40, 41]. The properties of branch-specific D-spikes are not yet well studied. Here, we investigated how D-spikes impact neuronal output and found that cutting certain oblique branches was sufficient to alter the neurons output profile. In summary, our results suggest that: (1) a single oblique branch among many neighboring branches in L5PNs can accommodate a D-spike; (2) a D-spike evoked at the branch manifests at the soma as an increase in the ADP; and (3) cutting an oblique branch that evoked a D-spike changes the ADP profile at the soma.

However, it remains an interesting question as to the role of branch-specific D-spikes in dendritic computation. One potential role is that D-spikes could be a mechanism to raise intracellular calcium levels and promote branch-specific plasticity [42]. Moreover, apart from their role on the dendritic computation, the resultant ADP at the soma could increase the probability of firing APs (if the ADP reaches firing threshold) and thus influence the excitability of the entire neuron.

Ultrafast Laser Scalpel

Cutting cellular features with minimal damage to neighboring structures and extracellular medium can be achieved using a tightly focused femtosecond-pulse laser. Cutting is confined within a small interaction volume at the focus where cascades of non-linear multiphoton-induced ablation processes occur [38, 43–47]. The dimensions of the interaction volume are defined by the diffraction limit of the optical system. Since the transverse diameter of the focus is in the order of ∼1 μm, the technique is able to prune dendrites with similar diameter while keeping the rest of the neuron’s dendritic tree and its surrounding extracellular matrix intact [26]. Maintaining a diffraction limited focus when cutting dendrites in deeper regions of the brain tissue can also be achieved by pre-compensating the optical aberrations caused by neuronal structures in the cortical tissue [48]. Thicker dendrites can also be pruned by transversely moving the focus across the dendrite during a longer exposure time. The technique has been used to perform axotomy, where axons of L5PNs were cut to study the generation of action potentials [49]. Note, however, that the cutting parameters, such as exposure time and laser power need to be regulated to facilitate an effective cut while avoiding damage of the surrounding tissue that can affect cell viability [26]. Here, we have successfully used the technique to prune and investigate the capacity of oblique dendrites to evoke D-spikes. After cutting, the cells regain their function and their ability to fire action potentials following current injection. While our experiments were done in acute brain slices (in vitro), recent work by Yamaguchi et al. [50] demonstrated the use of the technique for deep (up to ∼550 μm in depth from the surface of the mouse brain) focal dissection of apical and basal dendrites of L5PNs in vivo. Hence, in combination with other optical tools [39,51], the ultrafast laser scalpel can potentially be applied to study D-spikes in an intact rodent brain.

Conclusion

In conclusion, we propose a technique to analyze branch-specific D-spikes in cortical pyramidal neurons. D-spikes that occur only on specific branches are difficult to analyze especially in complex dendritic arborizations where the application of pharmacological agents or dendritic pinching technique could affect neighboring dendrites. We performed highly targeted dendrotomy via a non linear optical ablation process using a tightly focused femtosecond-pulse laser. We confined the interaction within the targeted dendrite thereby keeping the rest of the dendritic tree intact and the neuron remains viable. Our in vitro experiments were guided by numerical simulations, which confirmed our hypothesis that oblique branches have varying influence on neuronal function. While our proposed technique is irreversible, the technique can provide insights as to the role of certain dendrites in branch-specific computing mechanisms of individual neurons. Such goal can be achieved by performing systematic dendrotomy of oblique dendrites from different types of pyramidal neurons and classifying their corresponding outputs. Moreover, the ability to shape dendritic morphology to influence the output could also be used to study the broader roles of pyramidal neurons in brain circuits involving neuronal populations. D-spikes are critical computing attributes in pyramidal neurons and identifying their roles can facilitate a bottom-up approach to understand how the brain processes information during sensory perception, learning and memory.

Data Availability Statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

Ethics Statement

The handling of rats was performed according to the protocol approved by the Animal. Experimentation Ethics Committee of the Australian National University (Ethics protocol Number: A2018/35, Principal Investigator VRD; Understanding the role of apical obliques in neuronal function). Co-Investigator MC has undergone trainings for rat handling and surgery to prepare samples for the in vitro experiment. Sample preparation and data gathering were performed in a Physical Containment Level 2 (PC2) Laboratory.

Author Contributions

VD conceptualized the problem. VD built the microscope with advice from H-AB. MC performed the simulation and experiments. MC lead the writing of the manuscript with help from H-AB and VD.

Funding

This work was primarily funded by the National Health and Medical Research Council (PG1105944) and supported by the Australian Research Council (ARC) Discovery Project (DP140101555) and the ARC Centre of Excellence for Integrative Brain Function (CIBF).

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

We thank Greg Stuart for the comments and guidance he provided. We also thank John Bekkers for the valuable discussions on dendrotomy.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fphy.2020.600971/full#supplementary-material

References