Skip to main content
SpringerLink
Log in

Gamma-amino butyric acid effect in the alleviation of saline–alkaline stress conditions in rice

  • Original Paper
  • Published:
Cereal Research Communications Aims and scope Submit manuscript

Abstract

The present study was conducted in order to determine the effect of GABA on 2 rice cultivars, i.e., CSR 43 (tolerant) and Pusa 44 (susceptible) at seedling stage under 200 mM saline–alkaline stress (SAS) (molar ratio of 1:9:9:1 of NaCl/Na2SO4/Na2CO3/Na2HCO3) with and without GABA (1.5 mM) supplementation. Exogenous GABA application enhanced the protein content along with the activities of glutamate decarboxylase (GAD) and diamine oxidase (DAO) in cultivar CSR 43. Under SAS, the activities of GAD, DAO and contents of protein were found higher in CSR 43 as compared to Pusa 44 while reverse was observed under GABA + SAS. As compared to sole application of GABA/SAS, CSR 43 possessed higher proline content under GABA + SAS. Endogenous GABA contents were found to increase under SAS in both cultivars, whereas the levels remained higher in CSR 43. Thus, it is elucidated that the increased endogenous GABA by exogenous GABA treatment could improve stress tolerance of rice seedlings associated with GABA regulation in improving DAO activity along with proline content.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Aggarwal, A. 2018 Antioxidant response of rice (Oryza sativa L.) under saline-alkaline stress in the presence of gamma-amino butyric acid. M.Sc. Dissertation, Punjab Agricultural University Ludhiana, http://krishikosh.egranth.ac.in/handle/1/5810085017

  • Ahmed IM, Dai H, Zheng W, Cao F, Zhang G, Sun D, Wu F (2013) Genotypic differences in physiological characteristics in the tolerance to drought and salinity combined stress between Tibetan wild and cultivated barley. Plant Physiol Biochem 63:49–60

    Article  CAS  Google Scholar 

  • Akcay N, Bor M, Karabudak T, Ozdemir F, Turkan I (2012) Contribution of gamma amino butyric acid (GABA) to salt stress responses of Nicotiana sylvestris CMSII mutant and wild type plants. J Plant Physiol 169:452–458

    Article  CAS  Google Scholar 

  • Alan MK, Frank JT (2000) Gamma Aminobutyric Acid (GABA) and plant responses to stress. Crit Rev Plant Sci 19:479–509. https://doi.org/10.1016/S0735-2689(01)80006-X

    Article  Google Scholar 

  • Asthir B, Kumari M (2017) Modulation of antioxidant defense system and polyamine catabolism in rice leaves under two planting conditions. Cereal Res Commun 45(1):57–67

    Article  CAS  Google Scholar 

  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–216

    Article  CAS  Google Scholar 

  • Bouché N, Fromm H (2004) GABA in plants: Just a metabolite. Trends Plant Sci 9:110–115

    Article  Google Scholar 

  • Du W, Xiaoning N, Li M (2012) Effects of tutin and its derivatives on GAD and GABA-T in Pseudelatia separata (Lepidoptera : Noctuidae ). Rev Colomb Entomol 38:81–86

    CAS  Google Scholar 

  • Fait A, Fromm H, Walter D, Galili G, Fernie AR (2008) Highway or byway: the metabolic role of the GABA shunt in plants. Trends Plant Sci 13:14–19

    Article  CAS  Google Scholar 

  • Fait A, Nesi AN, Angelovici R, Lehmann M, Pham PA, Song L, Haslam RP, Napier JA, Galili G, Fernie AR (2011) Targeted enhancement of glutamate-to-γ-aminobutyrate conversion in Arabidopsis seeds affects carbon-nitrogen balance and storage reserves in a development-dependent manner. Plant Physiol 157:1026–1042

    Article  CAS  Google Scholar 

  • Fan L, Wu X, Tian Z, Jia K, Pan Y, Li J, Gao H (2015) Comparative proteomic analysis of gamma-aminobutyric acid responses in hypoxia-treated and untreated melon roots. Phytochem 116:28–37

    Article  CAS  Google Scholar 

  • FAO. 2009. High level expert forum- How to feed the world in 2050. Rome, Italy: economic and social development, food and agricultural organization of the United Nations.

  • Hu X, Xu Z, Xu W, Li J, Zhao N, Zhou Y (2015) Application of γ-aminobutyric acid demonstrates a protective role of polyamine and GABA metabolism in muskmelon seedlings under Ca (NO3)2 stress. Plant Physiol Biochem 92:1–10

    Article  CAS  Google Scholar 

  • Hu X, Zhang Y, Shi Y, Zhang Z, Zou Z, Zhang H, Zhao J (2012) Effect of exogenous spermidine on polyamine content and metabolism in tomato exposed to salinity-alkalinity mixed stress. Plant Physiol Biochem 57:200–209

    Article  CAS  Google Scholar 

  • Kaiser WM (2009) Effects of water deficit on photosynthetic capacity. Plant Physiol 71:142

    Article  Google Scholar 

  • Kaur, A. 2018. Role of gamma-amino butyric acid under saline-alkaline stress in rice (Oryza sativa L.). M. Sc. Dissertation, Punjab Agricultural University, Ludhiana, India, http://krishikosh.egranth.ac.in/handle/1/5810084815

  • Kinnersley AM, Turano FJ (2000) Gamma aminobutyric acid (GABA) and plant responses to stress. Crit Rev Plant Sci 19:479–509

    Article  CAS  Google Scholar 

  • Krishnan S, Laskowski K, Shukla V, Merewitz EB (2013) Mitigation of drought stress damage by exogenous application of a non-protein amino acid γ-aminobutyric acid on perennial ryegrass. J Am Soc Hortic Sci 138:358–366

    Article  Google Scholar 

  • Legocka J, Sobieszczuk-Nowicka E, Ludwicki D, Lehmann T (2017) Putrescine catabolism via DAO contributes to proline and GABA accumulation in roots of lupine seedlings growing under salt stress. Acta Soc Bot Pol 86:3549

    Article  Google Scholar 

  • Lin CC, Kao CH (2001) Cell wall peroxidase activity, hydrogen peroxide level and NaCl-inhibited root growth of rice seedlings. Plant Soil 230:135–143

    Article  CAS  Google Scholar 

  • Mattoo AK, Minocha SC, Minocha R, Handa AK (2010) Polyamines and cellular metabolism in plants: transgenic approaches reveal different responses to diamine putrescine versus higher polyamines spermidine and spermine. Amino Acids 38:405–413. https://doi.org/10.1007/s00726-009-0399-4

    Article  CAS  PubMed  Google Scholar 

  • Michaeli S, Fromm H (2015) Closing the loop on the GABA shunt in plants: Are GABA metabolism and signaling entwined? Front Plant Sci 6:419

    Article  Google Scholar 

  • Munns R, Tester M (2008) Mechanisms of salinity tolerance. Ann Rev Plant Biol 59:651–681

    Article  CAS  Google Scholar 

  • Nayyar H, Kaur R, Kaur S, Singh R (2014) γ-AminobutyricAcid (GABA) imparts partial protection from heat stress injury to rice seedlings by improving leaf turgor and up regulating osmoprotectants and antioxidants. J Plant Growth Regul. 33:408–419

    Article  CAS  Google Scholar 

  • Parida AK, Dagaonkar VS, Phalak MS, Aurangabadkar LP (2008) Differential responses of the enzymes involved in proline biosynthesis and degradation in drought tolerant and sensitive cotton genotypes during drought stress and recovery. Acta Physiol Plant 30:619–627. https://doi.org/10.1007/s11738-008-0157-3

    Article  CAS  Google Scholar 

  • Rangan P, Subramani R, Kumar R, Singh AK, Singh R (2014) Recent advances in polyamine metabolism and abiotic stress tolerance. Biomed Res Int 2014:239621. https://doi.org/10.1155/2014/239621

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Renault H, El Amrani A, Palanivelu R, Updegraff EP, Yu A, Renou JP, Preuss D, Bouchereau A, Deleu C (2011) GABA accumulation causes cell elongation defects and a decrease in expression of genes encoding secreted and cell wall-related proteins in Arabidopsis thaliana. Plant Cell Physiol 52:894–908

    Article  CAS  Google Scholar 

  • Renault H, Roussel V, El Amrani A, Arzel M, Renault D, Bouchereau A, Deleu C (2010) The Arabidopsis pop 2–1 mutant reveals the involvement of GABA transaminase in salt stress tolerance. BMC Plant Biol 10:20

    Article  Google Scholar 

  • Shang H, Cao S, Yang Z, Cai Y, Zheng Y (2011) Effect of exogenous γ-aminobutyric acid treatment on proline accumulation and chilling injury in peach fruit after long-term cold storage. J Agric Food Chem 59:1264–1268

    Article  CAS  Google Scholar 

  • Shi SQ, Shi Z, Jiang ZP, Qi LW, Sun XM, Li CX, Liu JF, Xiao WF, Zhang SG (2010) Effects of exogenous GABA on gene expression of Caragana intermedia roots under NaCl stress: regulatory roles for H2O2 and ethylene production. Plant Cell Environ 33:149–162

    Article  CAS  Google Scholar 

  • Skopelitis DS, Paranychianakis NV, Paschalidis KA, Pliakonis ED, Delis ID, Yakoumakis DI, Kouvarakis A, Papadakis AK, Stephanou EG, Roubelakis-Angelakis KA (2006) Abiotic stress generates ROS that signal expression of anionic glutamate dehydrogenases to form glutamate for proline synthesis in tobacco and grapevine. Plant Cell 18:2767–2781

    Article  CAS  Google Scholar 

  • Vijayakumari K, Puthur JT (2015) γ-Aminobutyric acid (GABA) priming enhances the osmotic stress tolerance in Piper nigrum Linn. plants subjected to PEG-induced stress. Plant Growth Regul 78:57–67. https://doi.org/10.1007/s10725-015-0074-6

    Article  CAS  Google Scholar 

  • Vijayakumari K, Jisha KC, Puthur JT (2016) GABA/BABA priming: A means for enhancing abiotic stress tolerance potential of plants with less energy investments on defence cache. Acta Physiol Plant 38:230

    Article  Google Scholar 

  • Xing SG, Jun YB, Hau ZW, Liang LY (2007) Higher accumulation of γ-aminobutyric acid induced by salt stress through stimulating the activity of diamine oxidases in Glycine max (L.) Merr.root. Plant Physiol Biochem 45:560–566

    Article  CAS  Google Scholar 

  • Yang R, Hui Q, Gu Z (2016) Effects of ABA and CaCl2 on GABA accumulation in fava bean germinating under hypoxia-NaCl stress. Biosci Biotechnol Biochem 80:540–546

    Article  CAS  Google Scholar 

  • Yin Y, Yang R, Han Y, Gu Z (2015) Comparative proteomic and physiological analyses reveal the protective effect of exogenous calcium on the germinating soybean response to salt stress. J Proteom 113:110–126

    Article  CAS  Google Scholar 

  • Zhang G, Bown AW (1997) The rapid determination of γ-aminobutyric acid. Phytochem 44:1007–1009

    Article  CAS  Google Scholar 

  • Zhang J, Zhang Y, Du Y, Chen S, Tang H (2011) Dynamic metabonomic responses of tobacco (Nicotiana tabacum) plants to salt stress. J Proteome Res 10:1904–1914

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Authors would like to thank Department of Plant Breeding and Genetics, P. A. U., Ludhiana, for providing seeds of rice cultivars CSR 43 and Pusa 44 and DSAASTAT for its free online availability for statistical analysis.

Author information

Authors and Affiliations

  1. Department of Biochemistry, College of Basic Sciences and Humanities, Punjab Agricultural University (PAU), Ludhiana, Punjab, India, 141004

    Ramanjeet Kaur, Vikramjit Kaur Zhawar, Gurpreet Kaur & Bavita Asthir

Authors
  1. Ramanjeet Kaur
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vikramjit Kaur Zhawar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gurpreet Kaur
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bavita Asthir
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Ramanjeet Kaur performed the experiments, collected data and analyzed it. Vikramjit Kaur Zhawar designed and supervised the experiments and provided technical expertise in the experimentation and data analysis and prepared the final manuscript. Gurpreet Kaur and Bavita Asthir helped in guiding experiments and preparation of the manuscript.

Corresponding author

Correspondence to Bavita Asthir.

Additional information

Communicated by Á. Gallé.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kaur, R., Zhawar, V.K., Kaur, G. et al. Gamma-amino butyric acid effect in the alleviation of saline–alkaline stress conditions in rice . CEREAL RESEARCH COMMUNICATIONS 49, 355–363 (2021). https://doi.org/10.1007/s42976-020-00119-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42976-020-00119-9

Keywords

Search

Navigation