Abstract
Adult neurogenesis has been profusely studied in central nervous system. However, its presence in enteric nervous system remains elusive although it has been recently demonstrated in mice and intimately linked to glial cells. Moreover, primary cilium is an important organelle in central adult neurogenesis. In the present study, we analysed some parallelisms between central and enteric nervous system (ENS) in humans based on ultrastructural and immunohistochemical techniques. Thus, we described the presence of primary cilia in some subtypes of glial cells and Interstitial Cells of Cajal (ICCs) and we performed 3-D reconstructions to better characterise their features. Besides, we studied the expression of several adult neurogenesis-related proteins. Immature neuron markers were found in human ENS, supporting the existence of adult neurogenesis. However, only ICCs showed proliferation markers. Hence, we propose a new paradigm where ICCs would constitute the original neural stem cells which, through asymmetrical cell division, would generate the new-born neurons.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
Authors would like to acknowledge the use of Servicio General de Apoyo a la Investigación - SAI, Universidad de Zaragoza. No specific funding was received for the experiments performed in this paper. Authors would like to acknowledge Dr. Adolfo Lopez de Munain for allowing PI access to his facilities to perform revision experiments.
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10571_2020_1017_MOESM1_ESM.tif
Electronic supplementary material 1 Supl. Fig. 1. Primary cilia in ICC. As our group already described, ICC show primary cilia. cr = ciliary rootlet, bb = basal body, a = axoneme (TIFF 151 kb)
Electronic supplementary material 2 Supl. Video 1. 3D reconstruction of a ciliated glial cell. Video projects 3D reconstruction of a glial cell showing primary cilia (AVI 2787 kb)
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Iruzubieta, P., Cantarero, I., Monzón, M. et al. Supporting Evidence of Human Enteric Nervous System Adult Neurogenesis: Presence of Primary Cilia and Adult Neurogenesis Markers. Cell Mol Neurobiol 42, 473–481 (2022). https://doi.org/10.1007/s10571-020-01017-8
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DOI: https://doi.org/10.1007/s10571-020-01017-8