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Expression of Epithelial Cell Adhesion Molecule (EpCAM) in Tumor Spheroids of Human Colorectal Adenocarcinoma Cells

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Bulletin of Experimental Biology and Medicine Aims and scope

We studied the formation of spheroids by Caco-2, SW480, and HCT116 human colorectal adenocarcinoma cell lines under low-adhesion culturing conditions. Of these three cell lines, only HCT116 formed stable tumor spheroids. Flow cytometry analysis of 19 surface markers in monolayer HCT116 culture and spheroids formed by these cells revealed considerable similarity of the expression profiles in these two culturing modes. The only exception was EpCAM molecule: its expression in spheroids was 3-fold higher than in the monolayer culture. Scanning confocal laser microscopy showed equal EpCAM distribution in the inner mass of the spheroids.

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Authors and Affiliations

  1. V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia

    A. M. Gisina, Ya. S. Kim, A. V. Tsvetkova, I. V. Vakhrushev, K. N. Yarygin & A. Yu. Lupatov

  2. National University of Science and Technology MISIS, Moscow, Russia

    A. N. Gabashvili

Authors
  1. A. M. Gisina
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  2. Ya. S. Kim
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  3. A. N. Gabashvili
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  4. A. V. Tsvetkova
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  5. I. V. Vakhrushev
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  6. K. N. Yarygin
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  7. A. Yu. Lupatov
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Correspondence to A. M. Gisina.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 178-184, September, 2020

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Gisina, A.M., Kim, Y.S., Gabashvili, A.N. et al. Expression of Epithelial Cell Adhesion Molecule (EpCAM) in Tumor Spheroids of Human Colorectal Adenocarcinoma Cells. Bull Exp Biol Med 170, 135–141 (2020). https://doi.org/10.1007/s10517-020-05018-x

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  • DOI: https://doi.org/10.1007/s10517-020-05018-x

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