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Efficacy of a commercial test kit to determine early pregnancy in cows using whole blood and blood serum

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Abstract

The objective of this study was to determine the efficacy of the Fassisi®BoviPreg visual test kit (Fassisi®BoviPreg) in determining early pregnancies in cows by measuring the pregnancy-associated glycoprotein (PAG) in whole blood and blood serum. The study was conducted on 50 cows, the artificial insemination (AI) dates of which were designated as day 0. Pregnancy diagnosis was performed with transrectal ultrasonography (USG), and serum samples were simultaneously collected and used with Fassisi®BoviPreg to determine pregnancies on 30 days after AI. The results of the Fassisi®BoviPreg test on serum and whole blood samples, respectively, on 30 days after AI were as follows: sensitivity, 61.54% and 50.0%; specificity, 79.17% and 75%; accuracy, 70.0% and 62.0%; positive predictive values, 76.2% and 68.4%; negative predictive values, 65.5% and 58.1%; false-positive diagnoses, 23.8% and 31.6%; and false-negative diagnoses, 34.5% and 41.9%. On day 50 after AI, sensitivities were 63.64% and 50.0%, specificities were 100.0% and 100.0%, and accuracies were 75.0% and 65.62% in serum and whole blood, respectively. Higher pregnancy rates were obtained using Fassisi®BoviPreg in cows with ≥ 5 ng/mL P4 (P < 0.001). In conclusion, the results from the Fassisi®BoviPreg tests on cows on 30 and 50 days after AI showed that the use of blood serum is more accurate and suitable than that of whole blood. The results also showed a higher confidence level in specificity 50 days after AI.

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Funding

This study is the first author’s master thesis and was financially supported by Kafkas University Scientific Research Coordination, Kars, Turkey (Project Number 2016-TS-59).

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Authors and Affiliations

  1. Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Kafkas University, 36100, Kars, Turkey

    Ozan Karakuş, Cihan Kaçar, Mushap Kuru, Semra Kaya & Murat Can Demir

  2. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Kafkas University, 36100, Kars, Turkey

    Umut Çağın Arı & Yavuz Öztürkler

  3. Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Harran University, 63200, Şanlıurfa, Turkey

    Abuzer Kafar Zonturlu

Authors
  1. Ozan Karakuş
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  2. Cihan Kaçar
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  3. Mushap Kuru
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  4. Semra Kaya
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  5. Murat Can Demir
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  6. Umut Çağın Arı
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  7. Abuzer Kafar Zonturlu
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  8. Yavuz Öztürkler
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Correspondence to Mushap Kuru.

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Karakuş, O., Kaçar, C., Kuru, M. et al. Efficacy of a commercial test kit to determine early pregnancy in cows using whole blood and blood serum. Trop Anim Health Prod 53, 28 (2021). https://doi.org/10.1007/s11250-020-02428-x

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