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A Rapid and reliable thin-layer chromatographic method for the simultaneous estimation of celecoxib and diacerein in their binary mixture using nanosilica gel plate

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Abstract

A novel, rapid, and sensitive stability-indicating high-performance thin-layer chromatography method was developed and validated for the simultaneous determination of celecoxib (CEL) and diacerein (DIA) in bulk and a capsule binary mixture. The complete separation was achieved by the proposed method on nanosilica gel F254 plates using n-hexane‒ethyl acetate‒tetrahydrofuran‒glacial acetic acid (7:3:1:0.8, V/V) as the mobile phase. The chromatographic bands were visualized using short-wave ultraviolet light, and the amount of CEL and DIA was determined by scanning densitometry at 254 nm using the peak area. The RF values were 0.59 and 0.37 for CEL and DIA, respectively. From regression plots, it was found that good linearity was achieved in the concentration ranges of 20.00–320.00 ng/band and 5.00–80.00 ng/band with mean percentage recoveries 99.86 ± 0.70 and 99.64 ± 0.96 for CEL and DIA, respectively. The proposed method was validated according to the International Conference on Harmonisation guidelines. The statistical analysis of the results revealed high accuracy and precision, the suggested procedures could be used as a stability-indicating assay for the simultaneous determination of CEL and DIA in bulk drug and their capsule binary mixture in the presence of their degradation products.

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Abbreviations

CEL:

Celecoxib

DIA:

Diacerein

THF:

Tetrahydrofuran

SD:

Standard deviation

RSD:

Relative standard deviation

SE:

Standard error

R F :

Retention factor

LOQ:

Limit of quantification

LOD:

Limit of detection

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Authors and Affiliations

  1. Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, Ein Helwan, Cairo, 11795, Egypt

    Mohamed Mohamed Salem Rizk, Safaa Shafik Toubar, Emad Ramzy Abd almalak Gadallah & Marwa Ibrahim Mohamed Helmy

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  1. Mohamed Mohamed Salem Rizk
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  2. Safaa Shafik Toubar
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  3. Emad Ramzy Abd almalak Gadallah
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  4. Marwa Ibrahim Mohamed Helmy
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Correspondence to Marwa Ibrahim Mohamed Helmy.

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Rizk, M.M.S., Toubar, S.S., Gadallah, E.R.A. et al. A Rapid and reliable thin-layer chromatographic method for the simultaneous estimation of celecoxib and diacerein in their binary mixture using nanosilica gel plate. JPC-J Planar Chromat 33, 511–522 (2020). https://doi.org/10.1007/s00764-020-00064-7

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  • DOI: https://doi.org/10.1007/s00764-020-00064-7

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