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Bioactivity-Oriented Purification of Polyphenols from Cinnamomum cassia Presl. with Anti-Proliferation Effects on Colorectal Cancer Cells

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Abstract

Cinnamomum cassia Presl. (CCP) is a popular natural spice possessing various pharmacological properties. We obtained polyphenol-rich fraction (CCP-P) from CCP by bioactivity-oriented purification method and evaluated its Wnt signaling inhibition activity. Firstly, the phenolic components were identified as the main bioactive compounds with anti-colorectal cancer activity. Then, we compared the anti-colorectal cancer activity of CCP extract obtained from different solvent by cell morphology alteration and EdU assay. Ethanol extract showed higher antiproliferative activity compared to water extract on HCT116 cells, with proliferating cells reducing to 41.12 and 21.83% at 156.00 μg GAE/mL, respectively. Next, separation and enrichment of polyphenols from ethanol extract was performed on AB-8 macroporous resins under optimal conditions. Further evaluation of the CCP-P bioactivity revealed that it exerted more potent antiproliferative activity on RKO and HCT116 cells, showing higher selectivity for Wnt-dependent colorectal cancer cells (CRCs). Ten major polyphenols were identified in the CCP-P by UPLC-ESI-MS/MS. In summary, this study presents evidence that CCP-derived polyphenols are promising potential candidates as functional food ingredients against CRC.

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Data Availability

All data and models generated or used during the study appear in the submitted article.

Abbreviations

BV:

Bed volume

CCP:

Cinnamomum cassia Presl

CCP-P:

Cinnamomum cassia Presl. polyphenols rich faction

CRC:

Colorectal cancer

CRCs:

Colorectal cancer cells

GAE:

Gallic acid equivalents

MTT:

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

TPC:

Total phenolic content

Wnt:

Wingless / Integrated

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Funding

National Natural Science Foundation of China (No. 31671804), the National Key R&D Program of China (2018YFD0901101) and Research and Development Program in Key Areas of Guangdong Province (2019B020210002).

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Authors and Affiliations

  1. School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, Guangdong, China

    Li Gao, Na Gou, Erdong Yuan & Jiaoyan Ren

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  1. Li Gao
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  2. Na Gou
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  3. Erdong Yuan
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Contributions

Li Gao performed purification, cell culture and cell experiments and written the manuscript. Na Gou performed extraction and EdU assay. Erdong Yuan revised the manuscript. Jiaoyan Ren designed experiment.

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Correspondence to Jiaoyan Ren.

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Gao, L., Gou, N., Yuan, E. et al. Bioactivity-Oriented Purification of Polyphenols from Cinnamomum cassia Presl. with Anti-Proliferation Effects on Colorectal Cancer Cells. Plant Foods Hum Nutr 75, 561–568 (2020). https://doi.org/10.1007/s11130-020-00846-8

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