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Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient

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Abstract

In the last years, an increasing number of untreatable infections caused by drug-resistant microbes have impacted the health care system. Worldwide, infections caused by carbapenem-resistant (CR) Gram-negative bacilli have dramatically increased. Among the CR-Gram-negative bacilli, those producing carbapenemases, such as NDM-1, are the main concern. Different Enterobacterales harboring NDM-1 have been reported lately. Providencia stuartii, a member of the Morganellaceae family, is ubiquitous in the environment, but is also known to cause nosocomial infections. Here we describe the genomic analysis of two NDM-1- producing P. stuartii strains recovered from the same patient as well as other carbapenem resistant strains recovered from the same hospital. As a result of the genomic analysis thirteen resistance genes, including three to β-lactams (blaOXA-1, blaTEM-1, blaNDM-1), four to aminoglycosides (aphA6, aac(3)-IId, aac(2′)-Ia, aac(6′)-Ib-cr5), one to sulfonamides (sul1), two to chloramphenicol (catB3, catA3), one to rifampicin, one to bleomycin (ble), and one to tetracycline (tet(B)) were found. Moreover, a variety of mobile genetic elements, such as insertion sequences, plasmids and phage- related sequences, were found within P. stuartii genomes. The spread of carbapenem-resistant isolates remains a significant clinical and public health concern. Therefore, we considered that the detection of CR isolates is an essential step in addressing this problem.

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Acknowledgements

Part of the authors’ work was supported by NIH SC3GM125556 to MSR. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. J.S.F. was supported by grant MHIRT 2T37MD001368 from the National Institute on Minority Health and Health Disparities, National Institute of Health. SM is recipient of a postdoctoral fellowship from CONICET. C.Q and G.M.T. are members of the CIC from CONICET.

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Author notes

  1. Amparo Hoard, Sabrina Montaña have contributed equally to this work.

Authors and Affiliations

  1. Center for Applied Biotechnology Studies, Department of Biological Science, California State University Fullerton, Fullerton, CA, USA

    Amparo Hoard, Alessandro Moriano, Jennifer S. Fernandez & Maria Soledad Ramirez

  2. Laboratorio de Bacteriología Clínica, Departamento de Bioquímica Clínica, Facultad de Farmacia Y Bioquímica, Hospital de Clínicas José de San Martín, Buenos Aires, Argentina

    Sabrina Montaña & German M. Traglia

  3. Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas Y Tecnológicas, Instituto de Investigaciones en Microbiología Y Parasitología Médica (IMPAM), Facultad de Medicina, Paraguay 2155- C1121ABG, Buenos Aires, Argentina

    Cecilia Quiroga

  4. Laboratorio de Bacteriologia, Hospital Interzonal de Agudos Eva Perón, Provincia de Buenos Aires, San Martín, Argentina

    Agustina Franchi, Emilia Cohen, Cecilia Corigliano & Marisa Almuzara

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  1. Amparo Hoard
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Contributions

(1) Conception and design of the study: AH, SM, AM, JSF, GMT, CQ, MA, MSR; (2) Acquisition of data: AH, SM, AM, JSF, GMT, AF, EC, CC, MA, MSR; (3) Analysis of data: AH, SM, GMT, CQ, MSR; (4) Drafting and revision of manuscript: AH, SM, GMT, CQ, MA, MSR.

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Correspondence to Maria Soledad Ramirez.

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All procedures performed in this study were in accordance with the ethical standards of the Hospital Eva Peron, Buenos Aires, Argentina, the 1964 Helsinki Declaration, its later amendments and the National Law on the protection of personal data No 25.326.

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Informed consent was obtained from the patient involved in the study.

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Hoard, A., Montaña, S., Moriano, A. et al. Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient. Curr Microbiol 77, 4029–4036 (2020). https://doi.org/10.1007/s00284-020-02242-6

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