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Properties of immobilized MAS1-H108A lipase and its application in the efficient synthesis of n-3 PUFA-rich triacylglycerols

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Abstract

This study reports the properties of immobilized MAS1-H108A lipase from marine Streptomyces sp. strain W007 on XAD1180 resin and its application in the synthesis of n-3 polyunsaturated fatty acids (PUFA)-rich triacylglycerols (TAG) for the first time. It was found that the optimal temperature and pH for both immobilized MAS1-H108A lipase and free lipase MAS1-H108A were 70 °C and 7.0, respectively. However, immobilized MAS1-H108A lipase exhibited higher thermostability when compared with free lipase MAS1-H108A. It was also interesting that both immobilized MAS1-H108A lipase and free lipase MAS1-H108A showed no regiospecificity in the hydrolysis of triolein. Subsequently, immobilized MAS1-H108A lipase and free lipase MAS1-H108A were employed to catalyze glycerolysis of n-3 PUFA-rich ethyl esters (EE) and esterification of n-3 PUFA with glycerol under vacuum in the solvent-free system. The results showed that n-3 PUFA-rich TAG were synthesized efficiently by non-regiospecific immobilized MAS1-H108A lipase and TAG contents separately reached 92.07% and 76.13% during the esterification and glycerolysis reactions, which were significantly higher than those (71.82% and 39.62%, respectively) obtained by free lipase MAS1-H108A. Besides, TAG exhibited similar n-3 PUFA composition to the substrate. These findings indicated that non-regiospecific immobilized MAS1-H108A lipase is a promising and efficient biocatalyst for the industrial synthesis of n-3 PUFA-rich TAG.

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Acknowledgements

This work was supported by the National Key R & D Program of China (2018YFC0311104), National Natural Science Foundation of China (31801462), National Science Fund for Distinguished Young Scholars (31725022), Guangdong MEPP Fund (NO.GDOE[2019]A20), Education and Research Project of Young and Middle-aged Teachers of Fujian Province (JT180467), the Cultivation Program for the Outstanding Young Scientific Research Talents of Fujian Province University (2018), Science and Technology Department of Putian (2018NP2003), Natural Science Foundation of Fujian Province (2020J05211, 2018J05063), Science and Technology Planning project of Putian University (2018055).

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Authors and Affiliations

  1. College of Environmental and Biological Engineering, Putian University, Putian, 351100, China

    Xiumei Wang & Xiaoxu Zhao

  2. Fujian Provincial Key Laboratory of Ecology-Toxicological Effects & Control for Emerging Contaminants, Putian, 351100, China

    Xiumei Wang & Xiaoxu Zhao

  3. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China

    Xiumei Wang, Zexin Zhao & Bo Yang

  4. College of Food Science, Southwest University, Chongqing, 400715, China

    Xiaoli Qin

  5. Guangdong Research Center of Lipid Science and Applied Engineering Technology, School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China

    Yonghua Wang

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  1. Xiumei Wang
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Wang, X., Zhao, X., Qin, X. et al. Properties of immobilized MAS1-H108A lipase and its application in the efficient synthesis of n-3 PUFA-rich triacylglycerols. Bioprocess Biosyst Eng 44, 575–584 (2021). https://doi.org/10.1007/s00449-020-02470-x

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