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Validation of a UV-Spectrophotometric Method for Quantitative Determination of Paclitaxel in a Targeted Delivery System Based on Poly(Lactic–Glycolic Acid) Copolymer Nanoparticles

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Pharmaceutical Chemistry Journal Aims and scope

A UV spectrophotometric method for quantitative determination of paclitaxel in a conjugate of poly(lactic-glycolic acid) (PLGA) copolymer nanoparticles and a protein vector (recombinant third domain of alpha-fetoprotein) was developed. Paclitaxel was determined at wavelength 260 ± 2 nm. The proposed method showed specificity, linearity (R2 = 0.9948) in the range 80 – 120% of paclitaxel nominal load in the polymer matrix (0.18 mg/mL), accuracy, and precision. The developed method could be recommended for inclusion in regulatory documentation for quality control of paclitaxel nanoparticles conjugated with a protein vector.

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References

  1. B. A. Weaver, Mol. Biol. Cell, 25, No. 18, 2677 – 2681 (2014).

    Article  Google Scholar 

  2. N. C. Kampan, M. T. Madondo, O. M. McNally, et al., BioMed Res. Int., 2015, 1 – 21 (2015).

    Article  Google Scholar 

  3. X. Du, A. R. Khan, and M. Fu, Int. J. Pharm., 542(1 – 2), 242 – 252 (2018).

    Article  CAS  Google Scholar 

  4. Z. Naz, S. Usman, K. Saleem, et al., Biomed. Res., 29(12), 2478 – 2483 (2018).

    Article  CAS  Google Scholar 

  5. E. S. Severin, Usp. Khim., 84(1), 43 – 60 (2015).

    Article  CAS  Google Scholar 

  6. United States Pharmacopeia 29, National Formulary 24 (2006).

  7. W. Deng, J. Qiu, S. Wang, et al., Int. J. Nanomed., 13, 439 (2018).

    Article  CAS  Google Scholar 

  8. C. G. Hiremath, G. B. Heggnnavar, M. Y. Kariduraganavar, et al., Prog. Biomater., 8, 1 – 14 (2019).

    Article  Google Scholar 

  9. S. Siddiqui, S. Paliwal, K. Kohli, et al., J. Chromatogr. Sep. Tech., 2, 3 – 4 (2012).

  10. A. Mittal, D. Chitkara, and N. Kumar, J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 855(2), 211 – 219 (2007).

    Article  CAS  Google Scholar 

  11. I. Badea, D. Ciutaru, L. Lazar, et al., J. Pharm. Biomed. Anal., 34(3), 501 – 507 (2004).

    Article  CAS  Google Scholar 

  12. E. D. Nikolskaya, O. A. Zhunina, N. G. Yabbarov, et al., Russ. J. Bioorg. Chem., 43, 278 – 285 (2017).

    Article  CAS  Google Scholar 

  13. M. B. Sokol, E. D. Nikolskaya, N. G. Yabbarov, et al., J. Biomed. Mater. Res. Part B, 107, 1150 – 1158 (2019).

  14. B. I. Kruglyi, E. D. Nikolskaya, and E. S. Severin, Oncopediatry, 3, 188 – 199 (2016).

    Article  Google Scholar 

  15. G. Song, M. H. Hayes, E. H. Novotny, et al., Naturwissenschaften, 98(1), 7 – 13 (2011).

    Article  CAS  Google Scholar 

  16. State Pharmacopoeia of the Russian Federation, XIVth Ed., Vol. 1, Moscow (2018).

  17. N. V. Yurgel’, Guideline for Pharmaceutical Industry Manufacturers [in Russian], Sport i Kul’tura, Moscow (2007), pp. 24 – 26.

    Google Scholar 

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Authors and Affiliations

  1. Russian Research Center for Molecular Diagnostics and Therapy, 8 Simferopol’skii Blvd, Moscow, 117638, Russia

    M. B. Sokol, Yu. V. Sycheva, N. G. Yabbarov, A. I. Zabolotskii, M. D. Mollaev, M. R. Faustova, O. G. Tereshchenko, M. V. Fomicheva & E. D. Nikol’skaya

  2. N. N. Semenov Federal Research Center of Chemical Physics, Russian Academy of Sciences, 4 Kosygina St, Moscow, 119991, Russia

    M. B. Sokol, N. G. Yabbarov, M. R. Faustova, M. V. Fomicheva & E. D. Nikol’skaya

  3. Department of Chemistry, M. V. Lomonosov Moscow State University, 1/3 Leninskii Gory, Moscow, 119991, Russia

    A. I. Zabolotskii

  4. M. V. Lomonosov Moscow Institute of Fine Chemical Technologies, MIREA – Russian Technological University, 86 Prosp. Vernadskogo, Moscow, 119571, Russia

    M. D. Mollaev & M. R. Faustova

Authors
  1. M. B. Sokol
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  2. Yu. V. Sycheva
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  3. N. G. Yabbarov
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  4. A. I. Zabolotskii
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  5. M. D. Mollaev
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  6. M. R. Faustova
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  7. O. G. Tereshchenko
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  8. M. V. Fomicheva
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  9. E. D. Nikol’skaya
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Corresponding author

Correspondence to M. B. Sokol.

Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 54, No. 8, pp. 47 – 51, August, 2020.

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Sokol, M.B., Sycheva, Y.V., Yabbarov, N.G. et al. Validation of a UV-Spectrophotometric Method for Quantitative Determination of Paclitaxel in a Targeted Delivery System Based on Poly(Lactic–Glycolic Acid) Copolymer Nanoparticles. Pharm Chem J 54, 846–850 (2020). https://doi.org/10.1007/s11094-020-02285-w

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  • DOI: https://doi.org/10.1007/s11094-020-02285-w

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