Issue 3, 2021
From the journal:

Biomaterials Science

In vitro and in vivo osteogenesis up-regulated by two-dimensional nanosheets through a macrophage-mediated pathway†

Haoming Liu, ORCID logo ‡ab   Gaojie Yang,‡c   Hao Yin,ab   Zhenxing Wang, ORCID logo ab   Chunyuan Chen,ab   Zhengzhao Liubd  and  Hui Xie*abdefg  

* Corresponding authors

a Department of Orthopedics, Xiangya Hospital, Central South University, Changsha 410008, China
E-mail: huixie@csu.edu.cn

b Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha 410008, China

c Department of Materials, Huazhong University of Science and Technology, Wuhan 430074, China

d Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha 410008, China

e National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China

f Hunan Key Laboratory of Organ Injury, Aging and Regenerative Medicine, Changsha 410008, China

g Hunan Key Laboratory of Bone Joint Degeneration and Injury, Changsha 410008, China

Abstract

Two-dimensional (2D) nanomaterials are attracting more and more interest in regenerative medicine due to their unique properties; however 2D biomimetic calcium mineral has not yet been developed and demonstrated application for bone tissue engineering. Here we described a novel calcium phosphate material with a 2D nanostructure that was synthesized using collagen and sodium alginate as the template. In vitro performance of the nanocrystalline material was evaluated, and we found that 2D CaP nanoparticles (NPs) enhanced the in vitro osteogenic differentiation of rat mesenchymal stem cells (rMSCs) through a macrophage-mediated signal pathway, when co-cultured with RAW 264.7 cells, rather than direct NP/stem cell interaction. A 2D topology structured surface was constructed by encapsulating the CaP nanomaterials in a gelatin hydrogel, which was demonstrated to be able to mediate in vivo ossification through a macrophage polarization related pathway in a femur defect rat model, and allowed the optimal therapeutic outcome compared to normal CaP counterparts. Our current work may have enlightened a new mechanism regarding NP-induced stem cell differentiation through immunoregulation, and the 2D CaP encapsulated hydrogel scaffold may serve as a potential alternative to autograft bone for orthopedic applications.

Graphical abstract: In vitro and in vivo osteogenesis up-regulated by two-dimensional nanosheets through a macrophage-mediated pathway

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/D0BM01596B
Article type
Paper
Submitted
18 Sep 2020
Accepted
30 Oct 2020
First published
09 Nov 2020

Biomater. Sci., 2021,9, 780-794

Permissions

Request permissions

In vitro and in vivo osteogenesis up-regulated by two-dimensional nanosheets through a macrophage-mediated pathway

H. Liu, G. Yang, H. Yin, Z. Wang, C. Chen, Z. Liu and H. Xie, Biomater. Sci., 2021, 9, 780 DOI: 10.1039/D0BM01596B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements