Abstract
Phytoseiid mites are efficient predators of mites and small pest insects. Understanding the dispersion and distribution pattern of phytoseiid mites is essential to promote the conservation of these natural enemies and support their use in biological control. Population genetic studies using molecular markers such as microsatellites have proved to be extremely informative to address questions about population structure and dispersion patterns of predatory mites. The objective of this work was to develop specific microsatellite markers for the predatory mite Phytoseiulus macropilis, aiming at improving field dispersion studies. For this purpose, the genomic DNA was extracted from the whole body of a pool of 260 adult females and used to build the genomic microsatellites-enriched library, using biotinylated probes (CT)8 and (GT)8. In total 26 pairs of primers were synthesized and screened across 30 adult females of P. macropilis for characterization. Seven loci were polymorphic, revealing from two to six alleles per locus. Cross amplifications were successfully obtained in the species Phytoseiulus persimilis, Amblyseius swirskii and Proprioseiopsis sp. The molecular markers obtained are the first developed for P. macropilis—they are effective for the detection and quantification of genetic variation, and show high transferability, thus can be used in genetic and molecular studies of this and other species of the same genus and also of close genera.
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Acknowledgements
The authors thank FAPESP (São Paulo Research Foundation) for the funding received for this research (Processes 2016/06919; 2017/50334-3) and for the doctoral scholarship to the first author (Process 2015/26288-6) and postdoctoral fellowship to the second author (Process 2018/18527-9). Our thanks also go to CNPq-Brazil (National Council for Scientific and Technological Development – Brazil) for providing the research fellowships to the third and fourth authors. This study was financed in part by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil) – Finance Code 001.
Funding
FAPESP (São Paulo Research Foundation) provided funding for this research (Processes 2016/06919-4; 2017/50334-3), scholarship to the first author (Process 2015/26288-6) and PD fellowship to the second author (Process 2018/18527-9). CNPq-Brazil (National Council for Scientific and Technological Development – Brazil) provided the research fellowships to the third and fourth authors (Process: 306852/2019-5). This study was financed in part by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil) – Finance Code 001.
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Queiroz, M.C.V., de Oliveira, F.A., de Souza, A.P. et al. Development of microsatellite markers for the predatory mite Phytoseiulus macropilis and cross-amplification in three other species of phytoseiid mites. Exp Appl Acarol 83, 1–12 (2021). https://doi.org/10.1007/s10493-020-00572-5
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DOI: https://doi.org/10.1007/s10493-020-00572-5