Issue 2, 2021
From the journal:

Analyst

A novel ratiometric fluorescent probe for the detection of mitochondrial pH dynamics during cell damage†

Gaoqing Fu,‡a   Guoxing Yin,‡b   Tingting Niu,a   Wei Wu,a   Hui Han,a   Haimin Chen ORCID logo *a  and  Peng Yin ORCID logo *b  

* Corresponding authors

a State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, Zhejiang 315211, China
E-mail: chenhaimin@nbu.edu.cn
Fax: (+86)57487609575
Tel: (+86)57487609575

b Key Laboratory of the Assembly and Application of Organic Functional Molecules of Hunan Province, Hunan Normal University, Changsha 410081, China
E-mail: yinpeng@hunnu.edu.cn

Abstract

A sensitive fluorescent probe (E)-4-(3-(benzo[d]thiazol-2-yl)-4-hydroxy-5-methylstyryl)-1-methylpyridin-1-ium iodide (HBTMP) for the monitoring of pH in mitochondria was rationally exploited. This novel probe exhibited remarkable pH-dependent behavior in the linear range of 5.5–8.0, along with a pKa value of 6.829 ± 0.02627. A large Stokes shift of 205 nm was obtained. This fluorescent probe demonstrated good biocompatibility and high sensitivity for detecting the dynamic changes in mitochondrial pH in living cells and zebrafish. The results of the CCCP (m-chlorophenyl hydrazone) treatment experiment indicated that the probe can effectively monitor changes in mitochondrial pH caused by cell damage.

Graphical abstract: A novel ratiometric fluorescent probe for the detection of mitochondrial pH dynamics during cell damage

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/D0AN01240H
Article type
Paper
Submitted
21 Jun 2020
Accepted
21 Sep 2020
First published
14 Nov 2020

Analyst, 2021,146, 620-627

Permissions

Request permissions

A novel ratiometric fluorescent probe for the detection of mitochondrial pH dynamics during cell damage

G. Fu, G. Yin, T. Niu, W. Wu, H. Han, H. Chen and P. Yin, Analyst, 2021, 146, 620 DOI: 10.1039/D0AN01240H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements