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Photovoltage generation by photosystem II core complexes immobilized onto a Millipore filter on an indium tin oxide electrode

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Abstract

The light-induced functioning of photosynthetic pigment-protein complex of photosystem II (PSII) is linked to the vectorial translocation of charges across the membrane, which results in the formation of voltage. Direct measurement of the light-induced voltage (∆V) generated by spinach oxygen-evolving PSII core complexes adsorbed onto a Millipore membrane filter (MF) on an indium tin oxide (ITO) electrode under continuous illumination has been performed. PSII was shown to participate in electron transfer from water to the ITO electrode, resulting in ∆V generation. No photovoltage was detected in PSII deprived of the water-oxidizing complex. The maximal and stable photoelectric signal was observed in the presence of disaccharide trehalose and 2,6-dichloro-1,4-benzoquinone, acting as a redox mediator between the primary quinone acceptor QA of PSII and electrode surface. Long time preservation of the steady-state photoactivity at room temperature in a simple in design ITO|PSII-MF|ITO system may be related to the retention of water molecules attached to the PSII surface in the presence of trehalose.

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Acknowledgements

This work was supported by the Russian Foundation for Basic Research grant № 17-00-00201 (complex project № 17-00-00218(K)) and FRCCP RAS state task (АААА-А19-119012990175- 9).

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  1. A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia

    Andrey A. Zaspa, Liya A. Vitukhnovskaya, Aida M. Mamedova, Alexey Yu. Semenov & Mahir D. Mamedov

  2. N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, Moscow, Russia

    Liya A. Vitukhnovskaya & Alexey Yu. Semenov

  3. First Moscow State Medical University, Moscow, Russia

    Aida M. Mamedova

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  1. Andrey A. Zaspa
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Zaspa, A.A., Vitukhnovskaya, L.A., Mamedova, A.M. et al. Photovoltage generation by photosystem II core complexes immobilized onto a Millipore filter on an indium tin oxide electrode. J Bioenerg Biomembr 52, 495–504 (2020). https://doi.org/10.1007/s10863-020-09857-1

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