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Whole genome sequencing to decipher the virulence phenotype of hypervirulent Klebsiella pneumoniae responsible for liver abscess, Marseille, France

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Abstract

We described three clinical cases of pyogenic liver abscess caused by hypervirulent Klebsiella pneumoniae (hvKp) successfully treated by prolonged antibiotherapy, in which one case was complicated by endophthalmitis. Whole genome sequencing helped to confirm the diagnosis of these hvKp strains, which belong to clonal complexes CC86 and CC23 and carried hvKp-associated genes (magA and/or rmpA). This syndrome is increasingly reported in France and Europe and raises questions about the source of infection.

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References

  1. Siu LK, Yeh K-M, Lin J-C, Fung C-P, Chang F-Y (2012) Klebsiella pneumoniae liver abscess: a new invasive syndrome. Lancet Infect Dis 12:881–887. https://doi.org/10.1016/S1473-3099(12)70205-0

    Article  PubMed  Google Scholar 

  2. Bialek-Davenet S, Criscuolo A, Ailloud F, Passet V, Jones L, Delannoy-Vieillard A-S et al (2014) Genomic definition of hypervirulent and multidrug-resistant Klebsiella pneumoniae clonal groups. Emerg Infect Dis 20:1812–1820. https://doi.org/10.3201/eid2011.140206

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Luo Y, Wang Y, Ye L, Yang J (2014) Molecular epidemiology and virulence factors of pyogenic liver abscess causing Klebsiella pneumoniae in China. Clin Microbiol Infect 20:O818–O824. https://doi.org/10.1111/1469-0691.12664

    Article  CAS  PubMed  Google Scholar 

  4. MMC L, Wyres KL, Duchêne S, Wick RR, Judd LM, Gan Y-H et al (2018) Population genomics of hypervirulent Klebsiella pneumoniae clonal-group 23 reveals early emergence and rapid global dissemination. Nat Commun 13(9):1–10. https://doi.org/10.1038/s41467-018-05114-7

    Article  CAS  Google Scholar 

  5. Merlet A, Cazanave C, Dutronc H, de Barbeyrac B, Brisse S, Dupon M (2012) Primary liver abscess due to CC23-K1 virulent clone of Klebsiella pneumoniae in France. Clin Microbiol Infect 18:E338–E339. https://doi.org/10.1111/j.1469-0691.2012.03953.x

    Article  CAS  PubMed  Google Scholar 

  6. Lan P, Shi Q, Zhang P, Chen Y, Yan R, Hua X et al (2020) Core genome allelic profiles of clinical Klebsiella pneumoniae strains using a random forest algorithm based on multilocus sequence typing scheme for hypervirulence analysis. J Infect Dis 16(221):S263–S271. https://doi.org/10.1093/infdis/jiz562

    Article  CAS  Google Scholar 

  7. Baron SA, Cassir N, Mékidèche T, Mlaga KD, Brouqui P, Rolain J-M (2019) Successful treatment and digestive decolonisation of a patient with osteitis caused by a carbapenemase-producing Klebsiella pneumoniae isolate harbouring both NDM-1 and OXA-48 enzymes. J Glob Antimicrob Resist 18:225–229. https://doi.org/10.1016/j.jgar.2019.06.001

    Article  PubMed  Google Scholar 

  8. Rossi B, Gasperini ML, Leflon-Guibout V, Gioanni A, de Lastours V, Rossi G et al (2018) Hypervirulent Klebsiella pneumoniae in cryptogenic liver abscesses, Paris. France Emerging Infect Dis 24:221–229. https://doi.org/10.3201/eid2402.170957

    Article  CAS  Google Scholar 

  9. Surgers L, Boyd A, Girard P-M, Arlet G, Decré D (2016) ESBL-producing strain of hypervirulent Klebsiella pneumoniae K2, France. Emerg Infect Dis 22:1687–1688. https://doi.org/10.3201/eid2209.160681

    Article  PubMed  PubMed Central  Google Scholar 

  10. Rafat C, Messika J, Barnaud G, Dufour N, Magdoud F, Billard-Pomarès T et al (2018) Hypervirulent Klebsiella pneumoniae, a 5-year study in a French ICU. J Med Microbiol 67:1083–1089. https://doi.org/10.1099/jmm.0.000788

    Article  CAS  PubMed  Google Scholar 

  11. Choby JE, Howard-Anderson J, Weiss DS (2020) Hypervirulent Klebsiella pneumoniae - clinical and molecular perspectives. J Intern Med 287:283–300. https://doi.org/10.1111/joim.13007

    Article  CAS  PubMed  Google Scholar 

  12. Pinpimai K, Roe WD, Biggs PJ, Dittmer KE, Michael SA (2018) Draft whole-genome sequences of five Klebsiella pneumoniae isolates from the Subantarctic Islands of New Zealand. Microbiol Resour Announc 7. https://doi.org/10.1128/MRA.01328-18

  13. Anzai EK, de Souza Júnior JC, Peruchi AR, Fonseca JM, Gumpl EK, Pignatari ACC et al (2017) First case report of non-human primates (Alouatta clamitans) with the hypervirulent Klebsiella pneumoniae serotype K1 strain ST 23: a possible emerging wildlife pathogen. J Med Primatol 46:337–342. https://doi.org/10.1111/jmp.12296

    Article  CAS  PubMed  Google Scholar 

  14. Harada S, Doi Y (2018) Hypervirulent Klebsiella pneumoniae: a call for Consensus Definition and International Collaboration. J Clin Microbiol 56. https://doi.org/10.1128/JCM.00959-18

  15. Beyrouthy R, Dalmasso G, Birer A, Robin F, Bonnet R (2020) Carbapenem resistance conferred by OXA-48 in K2-ST86 hypervirulent Klebsiella pneumoniae. France Emerging Infect Dis 26:1529–1533. https://doi.org/10.3201/eid2607.191490

    Article  CAS  Google Scholar 

  16. Du P, Zhang Y, Chen C (2018) Emergence of carbapenem-resistant hypervirulent Klebsiella pneumoniae. Lancet Infect Dis 18:23–24. https://doi.org/10.1016/S1473-3099(17)30625-4

    Article  PubMed  Google Scholar 

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Acknowledgments

We want to thank technicians of our microbiology laboratory and our genomic platform for technical assistance.

Funding

This work was supported by the French Government under the “Investissements d’avenir” (Investments for the Future) program managed by the Agence Nationale de la Recherche (ANR, fr: National Agency for Research), (reference: Méditerranée Infection 10-IAHU-03). This work was supported by Région Provence-Alpes-Côte d’Azur and European funding FEDER PRIMI.

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Authors and Affiliations

  1. IRD, APHM, MEPHI, Faculté de Médecine et de Pharmacie, Aix Marseille Univ, 19-21 boulevard Jean Moulin, 13385, Marseille, CEDEX 05, France

    Sophie Alexandra Baron, Léa-Marie Pascale, Matthieu Million, Linda Hadjadj & Jean-Marc Rolain

  2. IHU Méditerranée Infection, 19-21 boulevard Jean Moulin, 13385, Marseille, CEDEX 05, France

    Sophie Alexandra Baron, Léa-Marie Pascale, Matthieu Million, Linda Hadjadj & Jean-Marc Rolain

  3. Service de Médecine Interne, Hôpital La Timone, APHM, Marseille, France

    Antoine Briantais & Jean-Marc Durand

  4. IRD, APHM, MEPHI, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix Marseille Univ, 19-21 boulevard Jean Moulin, 13385, Marseille, CEDEX 05, France

    Jean-Marc Rolain

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  1. Sophie Alexandra Baron
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  2. Léa-Marie Pascale
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Correspondence to Jean-Marc Rolain.

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Baron, S.A., Pascale, LM., Million, M. et al. Whole genome sequencing to decipher the virulence phenotype of hypervirulent Klebsiella pneumoniae responsible for liver abscess, Marseille, France. Eur J Clin Microbiol Infect Dis 40, 1073–1077 (2021). https://doi.org/10.1007/s10096-020-04080-7

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  • DOI: https://doi.org/10.1007/s10096-020-04080-7

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