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Interleukin-8 Receptors CXCR1 and CXCR2 Are Not Expressed by Endothelial Colony-forming Cells

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Abstract

Endothelial colony-forming cells (ECFCs) are human vasculogenic cells described as potential cell therapy product and good candidates for being a vascular liquid biopsy. Since interleukin-8 (IL-8) is a main actor in senescence, its ability to interact with ECFCs has been explored. However, expression of CXCR1 and CXCR2, the two cellular receptors for IL-8, by ECFCs remain controversial as several teams published contradictory reports. Using complementary technical approaches, we have investigated the presence of these receptors on ECFCs isolated from cord blood. First, CXCR1 and CXCR2 were not detected on several clones of cord blood- endothelial colony-forming cell using different antibodies available, in contrast to well-known positive cells. We then compared the RT-PCR primers used in different papers to search for the presence of CXCR1 and CXCR2 mRNA and found that several primer pairs used could lead to non-specific DNA amplification. Last, we confirmed those results by RNA sequencing. CXCR1 and CXCR2 were not detected in ECFCs in contrary to human-induced pluripotent stem cell-derived endothelial cells (h-iECs). In conclusion, using three different approaches, we confirmed that CXCR1 and CXCR2 were not expressed at mRNA or protein level by ECFCs. Thus, IL-8 secretion by ECFCs, its effects in angiogenesis and their involvement in senescent process need to be reanalyzed according to this absence of CXCR-1 and − 2 in ECFCs.

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Acknowledgements

This work was supported by grants from the PROMEX STIFTUNG FUR DIE FORSCHUNG foundation that we deeply thank.

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Authors and Affiliations

  1. Innovative Therapies in Haemostasis, INSERM, Université de Paris, F-75006 , Paris, France

    Adeline Blandinières, Aurélien Philippe, Elisa Rossi, Grégoire Detriche, Nicolas Gendron, Pascale Gaussem, Coralie L. Guerin & David M. Smadja

  2. Service d’Hématologie et Laboratoire de Recherches Biochirugicales (Fondation Carpentier), AH-HP, Georges Pompidou European Hospital, F-75015, Paris, France

    Adeline Blandinières, Aurélien Philippe, Nicolas Gendron & David M. Smadja

  3. Department of Cardiac Surgery, Boston Children’s Hospital, Boston, MA, USA

    Xuechong Hong & Juan M. Melero-Martin

  4. Department of Surgery, Harvard Medical School, Boston, MA, USA

    Xuechong Hong & Juan M. Melero-Martin

  5. Department of Genetics, Université de Paris and Pharmacogenomics Unit, Institut Curie, Paris, France

    Ivan Bièche & Sophie Vacher

  6. Service d’Hématologie, AH-HP, Georges Pompidou European Hospital, F-75015, Paris, France

    Pascale Gaussem

  7. Plateforme de cytométrie, Institut Curie, F-75006, Paris, France

    Coralie L. Guerin

  8. Harvard Stem Cell Institute, Cambridge, MA, USA

    Juan M. Melero-Martin

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  1. Adeline Blandinières
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  2. Xuechong Hong
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Correspondence to David M. Smadja.

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Blandinières, A., Hong, X., Philippe, A. et al. Interleukin-8 Receptors CXCR1 and CXCR2 Are Not Expressed by Endothelial Colony-forming Cells. Stem Cell Rev and Rep 17, 628–638 (2021). https://doi.org/10.1007/s12015-020-10081-y

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