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Expression difference of P450–1 and P450–4 between G- and F-groups of Fusarium fujikuroi

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Abstract

Fusarium fujikuroi is the pathogen of rice bakanae disease and has been intensively studied for gibberellin (GA) production. F. fujikuroi is phylogenetically subclassified into G- and F-groups, which differ in GA and fumonisin production. A higher amount of GA is produced by the G-group than the F-group. A previous study found that the GA production difference between the G-group strain Gfc0801001 and the F-group strain Gfc0825009 was due to the GA gene cluster. In this study, higher expression of P450–1 and P450–4 in the GA gene cluster were detected in G-group strains than in F-group strains. The nucleotide sequence of the bidirectional promoter region between P450–1 and P450–4 and expression of P450–1 and P450–4 were compared between G- and F-group strains. The promoter sequence was identical among G-group strains while nucleotide substitutions were detected at various locations in F-group strains. Most F-group strains did not share the same substitutions. These results combined with the ancestral position of the F-group in the phylogenetic tree suggest that the population carrying highly expressed sequence generates the G-group rather than the low expression of P450–1 and P450–4 in the F-group due to a specific mutation occured in G-group.

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Acknowledgments

We thank Tomomi Katsu (Gifu University, Japan) and Ayako Usui (Gifu University, Japan) for technical support.

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The datasets generated during the current study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. The United Graduate School of Agricultural Science, Gifu University, Gifu, 501-1193, Japan

    W. X. Bao & S. Sultana

  2. Faculty of Applied Biological Science, Gifu University, Gifu, 501-1193, Japan

    S. Inagaki, S. Tatebayashi & M. Shimizu

  3. River Basin Research Center, Gifu University, Gifu, 501-1193, Japan

    K. Kageyama

  4. Life Science Research Center, Gifu University, Gifu, 501-1193, Japan

    H. Suga

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  1. W. X. Bao
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Correspondence to H. Suga.

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Bao, W.X., Inagaki, S., Tatebayashi, S. et al. Expression difference of P450–1 and P450–4 between G- and F-groups of Fusarium fujikuroi. Eur J Plant Pathol 159, 27–36 (2021). https://doi.org/10.1007/s10658-020-02133-3

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  • DOI: https://doi.org/10.1007/s10658-020-02133-3

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