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Genome-wide analysis of Hsp70 and Hsp100 gene families in Ziziphus jujuba

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Cell Stress and Chaperones Aims and scope

Abstract

The Ziziphus species are naturally tolerant to a range of abiotic stresses. Therefore, it is expected that they are an enriched source of genes conferring stress tolerance. Heat shock proteins (Hsps) play a significant role in plants in imparting tolerance against abiotic stress conditions. To get an insight into potential Hsp function in Ziziphus, we performed a genome-wide analysis and expression study of Hsp70 and Hsp100 gene families in Ziziphus jujuba. We identified 21 and 6 genes of the ZjHsp70 and ZjHsp100 families, respectively. Physiochemical properties, chromosomal location, gene structure, motifs, and protein domain organization were analysed for structural and functional characterization. We identified the contribution of tandem and segmental gene duplications in expansions of ZjHsp70s and ZjHsp100s in Z. jujuba. Promoter analysis suggested that ZjHsp70s and ZjHsp100s perform diverse functions related to abiotic stress. Furthermore, expression analyses revealed that most of the Z. jujuba Hsp genes are differentially expressed in response to heat, drought, and salinity stress. Our analyses suggested ZjHsp70-3, ZjHsp70-5, ZjHsp70-6, ZjHsp70-16, ZjHsp70-17, ZjHsp70-20, ZjHsp100-1, ZjHsp100-2, and ZjHsp100-3 are potential candidates for further functional analysis and with regard to breeding new more resilient strains. The present analysis laid the foundation for understanding the molecular mechanism of Hsps70 and Hsp100 gene families regulating abiotic stress tolerance in Z. jujuba.

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Acknowledgements

We gratefully acknowledge the National Phytotron Facility, IARI, for providing space for growing plants.

Funding

KP gratefully acknowledges the Indian Agricultural Research Institute (IARI) for the IARI-Junior Research Fellowship grant and the Department of Biotechnology (DBT), Government of India, for DBT-Senior research fellowship grant.

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Authors and Affiliations

  1. Division of Molecular Biology and Biotechnology, Indian Agriculture Research Institute, New Delhi, 110012, India

    Kishor Prabhakar Panzade

  2. Department of Plant Biotechnology, MGM College of Agricultural Biotechnology, Aurangabad, 431007, India

    Sonam S. Kale & Narendra R. Chavan

  3. Department of Plant Biotechnology, Ajeet Seeds Pvt. Ltd., Aurangabad, 431133, India

    Bhupal Hatzade

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  1. Kishor Prabhakar Panzade
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  2. Sonam S. Kale
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KPP conceived, designed experiments, performed the most of analysis, experiments, wrote and revised the manuscript. SSK conducted the analysis and help in manuscript writing. NRC and BH also help in manuscript writing.

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Correspondence to Kishor Prabhakar Panzade.

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Supplementary information

Table S1

Summary of all Hsp70 and Hsp100 gene sequences, functional domains and biochemical characteristics in Z. jujuba (XLSX 105 kb)

Table S2

Orthologous protein sequences of Hsp70 and Hsp100 gene family used for phylogenetic relationship analysis (XLSX 30 kb)

Table S3

List of primers used for qRT-PCR analysis of ZjHsp70 and ZjHsp100 gene family (XLSX 12 kb)

Table S4

Identified conserved motif sequences of ZjHsp70 and ZjHsp100 protein family in Z. jujuba (XLSX 11 kb)

Table S5

Summary of domains present in ZjHsp100 and ZjHsp70 proteins (XLSX 11 kb)

Table S6

Identified paralogous and orthologous pairs of ZjHsp70s and ZjHsp100s gene sequences of Z. jujuba, P. mume and A. thaliana (XLSX 11 kb)

Table S7

Details of cis-regulatory elements observed in ZjHsp70 and ZjHsp100 gene family (XLSX 20 kb)

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Panzade, K.P., Kale, S.S., Chavan, N.R. et al. Genome-wide analysis of Hsp70 and Hsp100 gene families in Ziziphus jujuba. Cell Stress and Chaperones 26, 341–353 (2021). https://doi.org/10.1007/s12192-020-01179-w

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