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Identification of exogenous ABA and heat stress tolerance in various cotton genotypes

Published online by Cambridge University Press:  12 November 2020

Yaping Guo Open the ORCID record for Yaping Guo [Opens in a new window] ,
Rong Fan ,
Fenglei Sun ,
Yanying Qu ,
Kai Zheng ,
Qin Chen  and
Quanjia Chen
Yaping Guo
Affiliation:
Engineering Research Center of Cotton of Ministry of Education, Country College of Agronomy, Xinjiang Agricultural University, Urumqi, Xinjiang, China
Rong Fan
Affiliation:
Engineering Research Center of Cotton of Ministry of Education, Country College of Agronomy, Xinjiang Agricultural University, Urumqi, Xinjiang, China
Fenglei Sun
Affiliation:
Engineering Research Center of Cotton of Ministry of Education, Country College of Agronomy, Xinjiang Agricultural University, Urumqi, Xinjiang, China
Yanying Qu
Affiliation:
Engineering Research Center of Cotton of Ministry of Education, Country College of Agronomy, Xinjiang Agricultural University, Urumqi, Xinjiang, China
Kai Zheng
Affiliation:
Engineering Research Center of Cotton of Ministry of Education, Country College of Agronomy, Xinjiang Agricultural University, Urumqi, Xinjiang, China
Qin Chen
Affiliation:
Engineering Research Center of Cotton of Ministry of Education, Country College of Agronomy, Xinjiang Agricultural University, Urumqi, Xinjiang, China
Quanjia Chen*
Affiliation:
Engineering Research Center of Cotton of Ministry of Education, Country College of Agronomy, Xinjiang Agricultural University, Urumqi, Xinjiang, China
*
*Corresponding author. E-mail: chqjia@126.com
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Abstract

Cotton fibre yield and quality are markedly influenced by drought and high-temperature stress. We examined the traits of the leaf stomata in 39 cotton genotypes subjected to exogenous phytohormone abscisic acid (ABA) signalling, electrolyte leakage under 40°C thermal stress, and relative GhHsfA, GhbZIP and GhHSP70 expression levels under two treatments. Stomatal density and area ranged from 66 to 182/mm2 and 663 to 1305 μm2, respectively. Under exogenous ABA signalling, the changes in stomatal aperture (ΔSAp) were in the range of 2.5–31.2%; ΔSAp and relative GhHsfA, GhbZIP and GhHSP70 expression levels were significantly correlated, respectively. Electrolyte leakage increased unequally among cotton genotypes after heat stress. The changes in electrolyte leakage (ΔEL) and relative GhHsfA, GhbZIP and GhHSP70 expression levels were very strongly correlated, respectively. Their relative expression levels could be used as references for the rapid identification of stress-tolerant cotton strains. Cluster analysis of the 39 cotton genotypes indicated that Xinluzao36, Shiyang1, shinong98-7 and Zhongmiansuo293 are heat- and drought-resistant. We integrated both analysis of physiological parameters and molecular methods to identify cotton varieties with the drought and heat tolerance, in order to provide a reference for the selection of materials and methods for the research and production of cotton.

Keywords

drought toleranceelectrolyte leakagegene expressionstomata
Type
Research Article
Information
Plant Genetic Resources , Volume 18 , Issue 6 , December 2020 , pp. 404 - 416
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press on behalf of NIAB

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References

Ahmad, A, Ilyas, MZ, Aslam, Z, Roman, M, Ali, A, Naeem, S, Nazar, M and Rehman, S (2020) Physiological screening of cotton (Gossypium hirsutum L.) genotypes against drought tolerance. Pure and Applied Biology 9: 140–147.Google Scholar
Bartlett, MK, Klein, T, Jansen, S, Choat, B and Sack, L (2016) The correlations and sequence of plant stomatal, hydraulic, and wilting responses to drought. Proceedings of the National Academy of Sciences 113: 13098–13103.CrossRefGoogle ScholarPubMed
Buckley, TN (2005) The control of stomata by water balance. New Phytologist 168: 275–292.CrossRefGoogle ScholarPubMed
Campos, PS, Quartin, V, Ramalho, JC and Nunes, MA (2003) Electrolyte leakage and lipid degradation account for cold sensitivity in leaves of Coffea sp. plants. Journal of Plant Physiology 160: 283–292.CrossRefGoogle ScholarPubMed
Clément, M, Leonhardt, N, Droillard, M, Reiter, I, Montillet, J, Genty, B, Laurière, C, Nussaume, L and Noël, LD (2011) The cytosolic/nuclear HSC70 and HSP90 molecular chaperones are important for stomatal closure and modulate abscisic acid-dependent physiological responses in Arabidopsis. Plant Physiology 156: 1481–1492.CrossRefGoogle ScholarPubMed
Danquah, A, de Zelicourt, A, Colcombet, J and Hirt, H (2013) The role of ABA and MAPK signaling pathways in plant abiotic stress responses. Biotechnology Advances 32: 40–52.CrossRefGoogle ScholarPubMed
Demidchik, V, Straltsova, D, Medvedev, SS, Pozhvanov, GA, Sokolik, A and Yurin, V (2014) Stress-induced electrolyte leakage: the role of K+-permeable channels and involvement in programmed cell death and metabolic adjustment. Journal of Experimental Botany 65: 1259–1270.CrossRefGoogle ScholarPubMed
Devi, MJ and Reddy, VR (2018) Transpiration response of cotton to vapor pressure deficit and its relationship with stomatal traits. Frontiers in Plant Science 9: 1–12.CrossRefGoogle ScholarPubMed
Du, Y-L, Wang, Z-Y, Fan, J-W, Turner, NC, He, J, Wang, T and Li, F-M (2013) Exogenous abscisic acid reduces water loss and improves antioxidant defence, desiccation tolerance and transpiration efficiency in two spring wheat cultivars subjected to a soil water deficit. Functional Plant Biology 40: 494–506.CrossRefGoogle ScholarPubMed
Gu, L, Jiang, T, Zhang, C, Li, X-D, Wang, C-M, Zhang, Y-M, Li, T, Dirk, LMA, Downie, AB and Zhao, T-Y (2019) Maize HSFA2 and HSBP2 antagonistically modulate raffinose biosynthesis and heat tolerance in Arabidopsis. The Plant Journal 100: 128–142.CrossRefGoogle ScholarPubMed
Hartung, W and Heilmeier, H (1993) Stomatal response to abscisic acid in natural environments. In: Jackson MB and Black CR (eds) Interacting Stresses on Plants in a Changing Climate, vol. 16, Berlin, Heidelberg: Springer-Verlag, pp. 525–526.CrossRefGoogle Scholar
Hatsugai, N and Katagiri, F (2018) Quantification of plant cell death by electrolyte leakage assay. Bio-Protocol 8: 1–7.CrossRefGoogle Scholar
He, J, Jin, Y, Palta, JA, Liu, H-Y, Chen, Z and Li, F-M (2019) Exogenous ABA induces osmotic adjustment, improves leaf water relations and water use efficiency, but not yield in soybean under water stress. Agronomy 9: 1–14.CrossRefGoogle Scholar
Hejnák, V, Tatar, Ö, Atasoy, GD, Martinková, J, Çelen, AE and Skalický, M (2016) Growth and photosynthesis of upland and pima cotton: response to drought and heat stress. Plant, Soil and Environment 61: 507–514.CrossRefGoogle Scholar
Hetherington, AM and Woodward, FI (2003) The role of stomata in sensing and driving environmental change. Nature 424: 901–908.CrossRefGoogle ScholarPubMed
Huang, X, Chen, M-H, Yang, L-T, Li, Y-R and Wu, J-M (2015) Effects of exogenous abscisic acid on cell membrane and endogenous hormone contents in leaves of sugarcane seedlings under cold stress. Sugar Tech: An International Journal of Sugar Crops & Related Industries 17: 59–64.CrossRefGoogle Scholar
Huang, Y-C, Niu, C-Y, Yang, C-R and Jinn, T-L (2016) The heat-stress factor HSFA6b connects ABA signaling and ABA-mediated heat responses. Plant Physiology 172: 1182–1199.Google ScholarPubMed
Jacob, P, Hirt, H and Bendahmane, A (2017) The heat-shock protein/chaperone network and multiple stress resistance. Plant Biotechnology Journal 15: 405–414.CrossRefGoogle ScholarPubMed
Kerr, TCC, Abdel-Mageed, H, Aleman, L, Lee, J, Payton, P, Cryer, D and Allen, RD (2018) Ectopic expression of two AREB/ABF orthologs increases drought tolerance in cotton (Gossypium hirsutum). Plant Cell and Environment 41: 898–907.CrossRefGoogle Scholar
Koiwai, H, Nakaminami, K, Seo, M, Mitsuhashi, W, Toyomasu, T and Koshiba, T (2004) Tissue-specific localization of an abscisic acid biosynthetic enzyme, AAO3, in Arabidopsis. Plant Physiology 134: 1697–1707.CrossRefGoogle ScholarPubMed
Kuromori, T, Miyaji, T, Yabuuchi, H, Shimizu, H, Sugimoto, E, Kamiya, A, Moriyama, Y and Shinozaki, K (2010) ABC transporter AtABCG25 is involved in abscisic acid transport and responses. Proceedings of the National Academy of Sciences 107: 2361–2366.CrossRefGoogle ScholarPubMed
Kuromori, T, Seo, M and Shinozaki, K (2018) ABA transport and plant water stress responses. Trends in Plant Science 23: 513–522.CrossRefGoogle ScholarPubMed
Lesk, C, Rowhani, P and Ramankutty, N (2016) Influence of extreme weather disasters on global crop production. Nature 529: 84–87.CrossRefGoogle ScholarPubMed
Li, H, Liu, S-S, Yi, C-Y, Wang, F, Zhou, J, Xia, X-J, Shi, K, Zhou, Y-H and Yu, J-Q (2014) Hydrogen peroxide mediates abscisic acid-induced HSP70 accumulation and heat tolerance in grafted cucumber plants. Plant, Cell & Environment 37: 2768–2780.CrossRefGoogle ScholarPubMed
Liang, C, Meng, Z, Meng, Z, Malik, W, Yan, R, Lwin, KM, Lin, F, Wang, Y, Sun, G, Zhou, T, Zhu, T, Li, J, Jin, S, Guo, S and Zhang, R (2016) GhABF2, a bZIP transcription factor, confers drought and salinity tolerance in cotton (Gossypium hirsutum L.). Scientific Reports 6: 1–14.CrossRefGoogle Scholar
Liu, H-C, Liao, H-T and Charng, Y-Y (2011) The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis. Plant Cell and Environment 34: 738–751.CrossRefGoogle ScholarPubMed
Livak, KJ and Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods (San Diego, Calif.) 25: 402–408.CrossRefGoogle Scholar
Mahmood, T, Khalid, S, Abdullah, M, Ahmed, Z, Shah, MKN, Ghafoor, A and Du, X (2020) Insights into drought stress signaling in plants and the molecular genetic basis of cotton drought tolerance. Cells 9: 1–30.Google Scholar
Malumpong, C, Cheabu, S, Mongkolsiriwatana, C, Detpittayanan, W and Vanavichit, A (2019) Spikelet fertility and heat shock transcription factor (Hsf) gene responses to heat stress in tolerant and susceptible rice (Oryza sativa L.) genotypes. The Journal of Agricultural Science 157: 283–299.CrossRefGoogle Scholar
Mao, H, Wang, H, Liu, S, Li, Z, Yang, X, Yan, J, Li, J, Tran, LSP and Qin, F (2015) A transposable element in a NAC Gene is associated with drought tolerance in maize seedlings. Nature Communications 6: 1–13.CrossRefGoogle Scholar
Moreno, AA, Mukhtar, MS, Blanco, F, Boatwright, JL, Moreno, I, Jordan, MR, Chen, Y-N, Brandizzi, F, Dong, X-N, Orellana, A and Pajerowska-Mukhtar, KM (2012) IRE1/bZIP60-mediated unfolded protein response plays distinct roles in plant immunity and abiotic stress responses. PLoS One 7: 1–15.CrossRefGoogle ScholarPubMed
Nakano, RT, Yamada, K, Bednarek, P, Nishimura, M and Hara-Nishimura, I (2014) ER bodies in plants of the Brassicales order: biogenesis and association with innate immunity. Frontiers in Plant Science 5: 1–18.CrossRefGoogle ScholarPubMed
Nejat, N and Mantri, N (2017) Plant immune system: crosstalk between responses to biotic and abiotic stresses the missing link in understanding plant defence. Current Issues in Molecular Biology 23: 1–16.CrossRefGoogle ScholarPubMed
Ohama, N, Kusakabe, K, Mizoi, J, Zhao, H, Kidokoro, S, Koizumi, S, Takahashi, F, Ishida, T, Yanagisawa, S, Shinozaki, K and Yamaguchi, SK (2016) The transcriptional cascade in the heat stress response of Arabidopsis is strictly regulated at the level of transcription factor expression. Plant Cell 28: 181–201.CrossRefGoogle ScholarPubMed
Parida, AK, Dagaonkar, VS, Phalak, MS, Umalkar, GV and Aurangabadkar, LP (2007) Alterations in photosynthetic pigments, protein and osmotic components in cotton genotypes subjected to short-term drought stress followed by recovery. Plant Biotechnology Reports 1: 37–48.CrossRefGoogle Scholar
Ryu, MY, Cho, SK and Kim, WT (2010) The Arabidopsis C3H2C3-type ring e3 ubiquitin ligase AtAIRP1 is a positive regulator of an abscisic acid-dependent response to drought stress. Plant Physiology 154: 1983–1997.CrossRefGoogle ScholarPubMed
Seo, M and Koshiba, T (2002) Complex regulation of ABA biosynthesis in plants. Trends in Plant Science 7: 41–48.CrossRefGoogle ScholarPubMed
Sripinyowanich, S, Klomsakul, P, Boonburapong, B, Bangyeekhun, T, Asami, T, Gu, H-Y, Buaboocha, T and Chadchawan, S (2013) Exogenous ABA induces salt tolerance in indica rice (Oryza sativa L.): the role of OsP5CS1 and OsP5CR gene expression during salt stress. Environmental and Experimental Botany 86: 94–105.CrossRefGoogle Scholar
Srivastava, R, Deng, Y and Howell, SH (2014) Stress sensing in plants by an ER stress sensor/transducer, bZIP28. Frontiers in Plant Science 5: 1–7.CrossRefGoogle ScholarPubMed
Tian, X-J, Wang, F, Zhao, Y, Lan, T-Y, Yu, K-H, Zhang, L-Y, Qin, Z, Hu, Z-R, Yao, Y-Y, Ni, Z-F, Sun, Q-X, Rossi, V, Peng, H-R and Xin, M-M (2020) Heat shock transcription factor A1b regulates heat tolerance in wheat and Arabidopsis through OPR3 and jasmonate signalling pathway. Plant Biotechnology Journal 18: 1109–1111.CrossRefGoogle ScholarPubMed
Trapero, MA, Morris, WL, Ducreux, LJM, McLean, K, Stephens, J, Torrance, L, Bryan, GJ, Hancock, RD and Taylor, MA (2018) Engineering heat tolerance in potato by temperature-dependent expression of a specific allele of heat-shock cognate 70. Plant Biotechnology Journal 16: 197–207.CrossRefGoogle Scholar
Ullah, A, Sun, H, Yang, X-Y and Zhang, X-L (2017) Drought coping strategies in cotton: increased crop per drop. Plant Biotechnology Journal 15: 271–284.CrossRefGoogle ScholarPubMed
Usman, MG, Rafii, MY, Martini, MY, Yusuff, OA, Ismail, MR and Miah, G (2017) Molecular analysis of Hsp70 mechanisms in plants and their function in response to stress. Biotechnology and Genetic Engineering Reviews 33: 26–39.CrossRefGoogle ScholarPubMed
Wan, J-X, Griffiths, R, Ying, J-F, McCourt, P and Huang, Y-F (2009) Development of drought-tolerant canola (Brassica napus L.) through genetic modulation of ABA-mediated stomatal responses. Crop Science 49: 1539–1554.CrossRefGoogle Scholar
Wang, X-L, Wang, H-W, Liu, S-X, Ferjani, A, Li, J-S, Yan, J-B, Yang, X-H and Qin, F (2016) Genetic variation in ZmVPP1 contributes to drought tolerance in maize seedlings. Nature Genetics 48: 1233–1241.CrossRefGoogle ScholarPubMed
Wang, C-L, Lu, G-Q, Hao, Y-Q, Guo, H-M, Guo, Y, Zhao, J and Cheng, H-M (2017) ABP9, a maize bZIP transcription factor, enhances tolerance to salt and drought in transgenic cotton. Planta 246: 453–469.CrossRefGoogle ScholarPubMed
Xiong, L-Z and Yang, Y-N (2003) Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase. Plant Cell 15: 745–759.CrossRefGoogle ScholarPubMed
Xu, Z-Z and Zhou, G-S (2008) Responses of leaf stomatal density to water status and its relationship with photosynthesis in a grass. Journal of Experimental Botany 59: 3317–3325.CrossRefGoogle ScholarPubMed
Zhang, X-Y, Zhang, X-Y, Liu, X-W, Shao, L-W, Sun, H-Y and Chen, S-Y (2016) Improving winter wheat performance by foliar spray of ABA and FA under water deficit conditions. Journal of Plant Growth Regulation 35: 83–96.CrossRefGoogle Scholar
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