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Detection reagents used for on-plate identification of organic pesticides in biological samples with preliminary separation by TLC/HPTLC

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Abstract

Many specific and non-specific chromogenic spray reagents have been used to detect organic pesticides (organophosphorus, organochlorine, carbamates, and pyrethroids) on thin-layer chromatographic (TLC)/high-performance thin-layer chromatographic (HPTLC) plates. To realize high sensitivity and improved selectivity, several chromogenic reagents have been introduced. The physical properties of organic pesticides reported so far are also presented in tabular form. The colors produced on TLC plates due to reactions taking place between pesticides and spray reagents are illustrated in the form of chromatograms, and reaction mechanism is also presented.

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Authors and Affiliations

  1. Physical Sciences Section, School of Sciences, Maulana Azad National Urdu University, Gachibowli, Hyderabad, TS, 500032, India

    Qasim Ullah & Nahid Fatema

  2. Department of Applied Chemistry, Zakir Hussain College of Engineering and Technology, Aligarh Muslim University, Aligarh, UP, 202002, India

    Ali Mohammad

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  1. Qasim Ullah
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  2. Nahid Fatema
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  3. Ali Mohammad
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Correspondence to Qasim Ullah.

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Qasim Ullah, Fatema, N. & Mohammad, A. Detection reagents used for on-plate identification of organic pesticides in biological samples with preliminary separation by TLC/HPTLC. JPC-J Planar Chromat 33, 533–546 (2020). https://doi.org/10.1007/s00764-020-00057-6

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