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The effect of vascular complications of diabetes mellitus on human umbilical cord tissue and the number of Wharton Jelly’s mesenchymal stem cells

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Abstract

The current study investigated the change in umbilical cord tissue and the number of markers of Wharton’s jelly mesenchymal stem cells (WJ-MSC) in pregnant women with gestational diabetes (GDM), with chronic diabetes who developed nephropathy as vascular complication (VC-PGDM), and healthy pregnant women as the control. The umbilical cords (UC) were investigated by the histomorphological method and the number of WJ-MSC were detected by flow-cytometry using the CD90, CD44, CD105, and CD73 markers in Wharton’s jelly (WJ) isolated from fresh umbilical cords. The number of positive cells for CD 90, CD44, CD105, and CD73 were found to be elevated in the GDM group, whereas it was significantly diminished in the VC-PGDM group (p = 0.001, p = 0.001, p = 0.001, and p = 0.001). The only histopathological sign in the GDM group were an increased number of pores in the Wharton jelly. Artery wall thickness/cord diamater ratio was increased, which indicates an increase of the artery wall thickness in the VC- PGDM group (p = 0.039 and p = 0.048). The increase in umbilical cord diameter and number of Wharton jelly mesenchymal stem cells in babies of gestational diabetic mothers was considered as an effect of macrosomia seen in babies of mothers with gestational diabetes. Vasculopathy, a long-term complication of diabetes, is known to affect all tissues by causing marked lower perfusion and hypoxia, as well as a decrease in the MSC number in our study.

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Funding

This study was funded by Gaziantep University Scientific Research Projects with project number TF.UT.18.04.

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Authors and Affiliations

  1. Department of Histology Embriology, Afyonkarahisar Health Science University Faculty of Medicine, Afyonkarahisar, Turkey

    Cigdem Karaca

  2. Department of Histology Embriology, Gaziantep Ünivercity Faculty of Medicine, Gaziantep, Turkey

    Nuray Bostancıeri

  3. Department of Obstetrics and Gynecology, Cengiz Gökcek Women’s and Children’s Hospital, Gaziantep, Turkey

    Ali Ovayolu

  4. Department of Medical Biochemistry, Gaziantep Ünivercity Faculty of Medicine, Gaziantep, Turkey

    Demet Tasdemir Kahraman

Authors
  1. Cigdem Karaca
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  2. Nuray Bostancıeri
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  3. Ali Ovayolu
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  4. Demet Tasdemir Kahraman
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Çiğdem Karaca, Nuray Bostancıeri, Ali Ovayolu and Demet Taşdemir Karahan. The first draft of the manuscript was written by Çiğdem Karaca and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Cigdem Karaca.

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The authors declare that they have no conflict of interest.

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Yes.

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All authors approved the publication.

Ethical approval

Permission was obtained with the decision of Gaziantep University Faculty of Medicine Medical Ethics Committee dated 24.07.2017 and numbered 2017/268. The procedures used in this study adhere to the tenets of the Declaration of Helsinki.

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Supplementary Figure 1 WJ views in all three groups respectively a) GDM b) VC PGDM c) Control (JPG 505 kb)

Supplementary Figure 2 Vessel wall thickness/umbilical cord diameter ratios in all groups (JPG 31 kb)

11033_2020_5965_MOESM3_ESM.jpg

Supplementary Figure 3 Graph comparing CD90, CD105, CD73 and CD44+ cell numbers. All values are represented as mean ± SD.). *Denote significant differences at the level of p<0.005 between the groups (JPG 47 kb)

(DOCX 20 kb)

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Karaca, C., Bostancıeri, N., Ovayolu, A. et al. The effect of vascular complications of diabetes mellitus on human umbilical cord tissue and the number of Wharton Jelly’s mesenchymal stem cells. Mol Biol Rep 47, 9313–9323 (2020). https://doi.org/10.1007/s11033-020-05965-8

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  • DOI: https://doi.org/10.1007/s11033-020-05965-8

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