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Association of HLA-DQ and IL13 gene variants with challenge-proven shrimp allergy in West Bengal, India

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Abstract

Little is known about genetic factors and mechanisms underlying shrimp allergy. Genome-wide association studies identified HLA class-II and IL13 genes as highly plausible candidates for shrimp allergy. The present study was designed to investigate potential associations of HLA-DQ rs9275596, IL13 rs20541, and IL13 rs1800925 polymorphisms with challenge-proven shrimp allergy using the data from 532 people of West Bengal, India; selected on basis of positive skin prick test, elevated specific IgE and medical history. Risk genotypes, i.e., HLA-DQ rs9275596 CC, IL13 rs20541 AA, and IL13 rs1800925 TT, were found to be significantly associated with challenge positive shrimp allergy (P = 0.04, 0.01, and 0.03, respectively). Distribution of genotypes for HLA-DQ and IL13 polymorphisms in allergic and control subjects showed significant difference between younger (20–40 years) and older (> 40 years) age group (P = 0.006). Risk genotypes significantly associated with elevated shrimp-specific IgE. IL13 TA haplotype significantly associated with shrimp allergy and elevated specific IgE (P = 0.02). Synergistic effect of IL13 TA haplotype–HLA-DQ rs9275596 CC genotype interaction significantly elevated specific IgE (P = 0.03). The present study suggests that HLA-DQ and IL13 polymorphisms pose major risk for shrimp allergic patients in West Bengal, India and thus could be helpful for early target-specific therapeutic intervention in near future.

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Data availability

The datasets generated and/or analyzed during the current study are not publicly available due to consent requirements to protect subjects’ privacy but are available from the corresponding author on reasonable request.

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Acknowledgements

The authors convey thanks to the patients who voluntarily participated in our study. We are also grateful to the lab professionals of the Allergy and Asthma Research Center, Kolkata, for their technical help. We are also thankful to the Principal, Barasat Govt. College for providing infrastructure facilities for this study.

Funding

Was provided by Council of Scientific and Industrial Research, Govt. of India (Sanction no. 08/606(0004)/2017-EMR-I dated 14/11/2017) and Department of Science and Technology, Govt. of West Bengal (Sanction No. 1170(Sanc.)/ST/P/S&T/1G-4/2016, dated 02.03.16).

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Authors and Affiliations

  1. Allergology and Applied Entomology Research Laboratory, Department of Zoology, University of Burdwan, Bardhaman, 713104, West Bengal, India

    Arghya Laha & Sanjoy Podder

  2. Department of Life Sciences, Presidency University, Kolkata, 700073, West Bengal, India

    Amlan Ghosh

  3. Allergy and Asthma Research Centre, Kolkata- 700029, West Bengal, India

    Saibal Moitra

  4. Post Graduate Department of Zoology, Krishnagar Government College, Krishnagar, 741101, West Bengal, India

    Himani Biswas

  5. Vice-Chancellor, University of Burdwan, Bardhaman, 713104, West Bengal, India

    Nimai Chandra Saha

  6. Post Graduate Department of Physics, Barasat Government College, Kolkata, 700124, West Bengal, India

    Srijit Bhattacharya

  7. Department of Zoology, University of Calcutta, Kolkata, 700019, West Bengal, India

    Goutam Kumar Saha

Authors
  1. Arghya Laha
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  2. Amlan Ghosh
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  3. Saibal Moitra
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  4. Himani Biswas
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  5. Nimai Chandra Saha
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  6. Srijit Bhattacharya
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  7. Goutam Kumar Saha
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  8. Sanjoy Podder
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Contributions

All the authors have made substantial contributions. Arghya Laha contributed to data compilation and wrote first draft of the manuscript. Amlan Ghosh designed the genotyping protocol for HLA-DQ rs9275596 and helps in LD analysis. Saibal Moitra diagnosed the disease, provided the sample, and supervised the oral food challenge. Himani Biswas helped in data collection. Srijit Bhattacharya did the statistical analysis. Nimai Chandra Saha, Goutam Kumar Saha, and Sanjoy Podder conceived the study design. Sanjoy Podder supervised the work, critically revised the manuscript, and made the final draft. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Sanjoy Podder.

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Competing interests

The authors declare that they have no competing interests.

Ethics approval and consent to participate

The study protocol was approved by the Clinical Research Ethics Committee, Allergy and Asthma Research Center, West Bengal, India (CREC-AARC Ref: 004/17). All the procedures performed in the present study involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Written informed consent was obtained from all included subjects and patient anonymity was preserved using methods approved by the Ethics Committee.

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Laha, A., Ghosh, A., Moitra, S. et al. Association of HLA-DQ and IL13 gene variants with challenge-proven shrimp allergy in West Bengal, India. Immunogenetics 72, 489–498 (2020). https://doi.org/10.1007/s00251-020-01185-3

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  • DOI: https://doi.org/10.1007/s00251-020-01185-3

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