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Effects of Ocean Acidification and Microplastics on Microflora Community Composition in the Digestive Tract of the Thick Shell Mussel Mytilus coruscus Through 16S RNA Gene Sequencing

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Abstract

Ocean acidification and microplastic pollution is a global environmental threat, this research evaluated the effects of ocean acidification and microplastics on mussel digestive tract microbial community. The 16S rRNA gene was sequenced to characterize the flora. Species diversity in the samples was assessed by clustering valid tags on 97% similarity. Bacteroidetes, Firmicutes and Proteobacteria were the three most abundant genera in the four groups, with Bacteroidetes showing the highest diversity. However, no differences in flora structure were evident under various treatments. Phylogenetic relationship analysis revealed Bacteroidetes and Firmicutes had the highest OTU diversity. The weighted UniFrac distance, principal coordinate analysis (PCoA), unweighted pair group method with arithmetic mean (UPGMA) cluster tree and analysis of molecular variance (AMOVA) evaluation results for all samples also showed that changes in pH and microplastics concentration did not significantly affect the microbial community structure in the mussel digestive tract. The results presented the no significant effects of ocean acidification and microplastics intake on mussel intestinal diversity.

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Acknowledgements

This work was supported by the natural Science Foundation of China (41706142).

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Author notes

  1. Liguo Yang and Linlan Lv have contributed equally to this work.

Authors and Affiliations

  1. Department of Ocean Technology, College of Chemistry and Biology Engineering, Yancheng Institute of Technology, Yancheng, 224051, China

    Linlan Lv & Yanming Sui

  2. Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China

    Liguo Yang & Yanming Sui

  3. International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai, China

    Youji Wang

  4. Faculty of Agricultural and Environmental Sciences, University of Rostock, Rostock, Germany

    Haojie Liu & Miaorun Wang

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  1. Liguo Yang
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  2. Linlan Lv
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  3. Haojie Liu
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  4. Miaorun Wang
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  5. Yanming Sui
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Correspondence to Yanming Sui or Youji Wang.

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Yang, L., Lv, L., Liu, H. et al. Effects of Ocean Acidification and Microplastics on Microflora Community Composition in the Digestive Tract of the Thick Shell Mussel Mytilus coruscus Through 16S RNA Gene Sequencing. Bull Environ Contam Toxicol 107, 616–625 (2021). https://doi.org/10.1007/s00128-020-03022-5

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