Abstract
The effects of several surfactants on the biosynthesis of β-1,3-D-glucan (β-glucan) and pullulan by Aureobasidium pullulans CCTCC M 2012259 were investigated, and Triton X-100 was found to decrease biomass formation but increase β-glucan and pullulan production. The addition of 5 g/L Triton X-100 to the fermentation medium and bioconversion broth significantly increased β-glucan production by 76.6% and 69.9%, respectively, when compared to the control without surfactant addition. To reveal the physiological mechanism underlying the effect of Triton X-100 on polysaccharides production, the cell morphology and viability, membrane permeability, key enzyme activities, and intracellular levels of UDPG, NADH, and ATP were determined. The results indicated that Triton X-100 increased the activities of key enzymes involved in β-glucan and pullulan biosynthesis, improved intracellular UDPG and energy supply, and accelerated the transportation rate of precursors across the cell membrane, all of which contributed to the enhanced production of β-glucan and pullulan. Moreover, a two-stage culture strategy with combined processes of batch fermentation and bioconversion was applied, and co-production of β-glucan and pullulan in the presence of 5 g/L Triton X-100 additions was further improved. The present study not only provides insights into the effect of surfactant on β-glucan and pullulan production but also presents a feasible approach for efficient production of analogue exopolysaccharides.
Key points
• Triton X-100 increased β-glucan and pullulan production under either batch fermentation or bioconversion.
• Triton X-100 increased the permeability of cell membrane and accelerated the transportation rate of precursors across cell membrane.
• Activities of key enzymes involved in β-glucan and pullulan biosynthesis were increased in the presence of Triton X-100.
• Intracellular UDPG levels and energy supply were improved by Triton X-100 addition.
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Funding
This work was supported by the Natural Science Foundation of Jiangsu Province (BK20181440), the National Natural Science Foundation of China (21776189), and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.
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DHW and GYW conceived and designed research. GLW, AUD, and YSQ conducted experiments. CLW and DHW analyzed the data. GLW and AUD wrote the manuscript. CLW and GYW reviewed and edited the manuscript. DHW and GYW administrated project and acquired funding. All authors read and approved the manuscript.
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Wang, GL., Din, A.U., Qiu, YS. et al. Triton X-100 improves co-production of β-1,3-D-glucan and pullulan by Aureobasidium pullulans. Appl Microbiol Biotechnol 104, 10685–10696 (2020). https://doi.org/10.1007/s00253-020-10992-3
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DOI: https://doi.org/10.1007/s00253-020-10992-3