Abstract
Antimicrobial peptides (AMPs) are recognized as promising safe alternatives to antibiotics for its low drug-resistance. Brevilaterin B, a newly discovered antimicrobial lipopeptide produced by Brevibacillus laterosporus S62-9, exhibits efficient antibacterial activity on Listeria monocytogenes with a minimum inhibitory concentration of 1 μg mL−1. The present research aimed to investigate the antibacterial mechanism of brevilaterin B against Listeria monocytogenes. Brevilaterin B caused membrane depolarization and the breakup of the cytomembrane as measured by 3,3-dipropylthiadicarbocyanine iodide and transmission electron microscopy, respectively. Using 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (7:3) as a model membrane, results proved that brevilaterin B could bind to liposomes, integrate into the lipid bilayer, and consequently increase the permeability of liposomes to calcein. The secondary structure of brevilaterin B also changed from an unstructured coil to a mainly β-sheet conformation as measured by circular dichroism. Brevilaterin B exhibits antibacterial activity by a membrane interaction mechanism, which provides a theoretical basis for using brevilaterin B as a promising natural and effective antimicrobial agent against pathogenic bacteria.
Key points
• Brevilaterin B exhibited antibacterial activity against Listeria monocytogenes.
• Brevilaterin B exhibited membrane interaction mechanism.
• Brevilaterin B showed conformational change when interacted with liposome.
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This research was financially supported by National Natural Science Foundation of China, grant numbers 31771951 & 31801510; the Beijing Natural Science Foundation, grant number KZ201810011016; and the Beijing Science and Technology Project, grant number Z181100009318005.
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LY carried out the experiments, analyzed data, and drafted the manuscript. HP participated in experiments. MA, CZ, and JY conceived and coordinated the study and drafted the manuscript. All authors have read and agreed to the published version of the manuscript.
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Liu, Y., Ma, A., Han, P. et al. Antibacterial mechanism of brevilaterin B: an amphiphilic lipopeptide targeting the membrane of Listeria monocytogenes. Appl Microbiol Biotechnol 104, 10531–10539 (2020). https://doi.org/10.1007/s00253-020-10993-2
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DOI: https://doi.org/10.1007/s00253-020-10993-2