Skip to main content
SpringerLink
Log in

Comparative quantitative phytochemical and HPTLC analysis of two Euphorbiaceae family plants under the name Dugdhika

  • Original Research Paper
  • Published:
JPC – Journal of Planar Chromatography – Modern TLC Aims and scope Submit manuscript

Abstract

Euphorbia hirta and Euphorbia thymifolia are plants with the same regional name “Dudheli” in Gujarat region, India. The present study has been conducted with comparative analysis of two plants, E. hirta and E. thymifolia, for total flavonoid content, total phenolic content, and quantification of biomarkers (rutin, gallic acid, and quercetin) by using high-performance thin-layer chromatography (HPTLC). Total phenolic contents (TPC) and total flavonoid contents (TFC) were evaluated by Folin‒Ciocalteu method and aluminum chloride colorimetric method, respectively. An improved HPTLC method was developed and validated for the simultaneous estimation of rutin, quercetin, and gallic acid. TPC was found more in the methanolic extract of E. hirta, and TFC was found almost the same in ethyl acetate extracts of both plants. Both plants showed the presence of rutin, quercetin, and gallic acid. The results of the HPTLC study provided a valuable bioactive marker content comparison between two species of the Euphorbiaceae family.

Graphic abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Availability of data and material

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

References

  1. Anonymous (2008a) Ayurvedic Pharmacopoeia of India, Part 1. Ministry of Health and Family Welfare, Department of AYUSH, New Delhi, Government of India, vol 5, pp 36–38

  2. Anonymous (2008b) Ayurvedic Pharmacopoeia of India, Part 1. Ministry of Health and Family Welfare, Department of AYUSH, New Delhi, Government of India, vol 6, p 62

  3. Anand V, Kausar J, Muthumani D, Hedina A, Sivasamy R (2016) Review of pharmacological and phytochemical activities of Euphorbia hirta Linn. Pharmacogn J 8(4):310–313. https://doi.org/10.5530/pj.2016.4.2

    Article  CAS  Google Scholar 

  4. Oparaocha ET, Okorie C (2009) In vivo evaluation of the antimalarial activity of three medicinal plants used in South Eastern Nigeria. J Herbs Spices Med Plants 15(1):121–128. https://doi.org/10.1080/10496470902797914

    Article  Google Scholar 

  5. Sashini DP, Uthpala AJ, Chanika DJ (2018) Potential use of Euphorbia hirta for dengue: a systematic review of scientific evidence. J Trop Med, Article ID: 2048530. https://doi.org/10.1155/2018/2048530

  6. Ogueke CC, Ogbulie JN, Okoli IC, Anyanwu BN (2007) Antibacterial activities and toxicological potentials of crude ethanolic extracts of Euphorbia hirta. J Am Sci 3(3):11–16

    Google Scholar 

  7. Johnson PB, Abdurahman EM, Tiam EA, Abdu-Aguye I, Hussaini IM (1999) Euphorbia hirta leaf extracts increase urine output and electrolytes in rats. J Ethnopharmacol 65(1):63–69. https://doi.org/10.1016/s0378-8741(98)00143-3

    Article  CAS  PubMed  Google Scholar 

  8. Subramanian SP, Bhuwaneshwari S, Prasath GS (2011) Anti-diabetic and anti-oxidant potentials of Euphorbia hirta leaves extract studied in streptozotocin-induced experimental diabetes in rats. Asian Pac J Trop Med 5:386–390. https://doi.org/10.4149/gpb_2011_03_278

    Article  CAS  Google Scholar 

  9. Rastogi R, Mehrotra B (2002) Compendium of Indian medicinal plants, vol 4. Central Drug Res Inst, Lucknow, pp 110–111

    Google Scholar 

  10. Kirtikar KR, Basu BD (1975) Indian medicinal plants, 2nd edn. Bishen Singh Mahendra Pal Singh, Dehradun, p 2199

    Google Scholar 

  11. Lin CC, Cheng HY, Yang CM, Lin TC (2002) Antioxidant and antiviral activities of Euphorbia thymifolia L. J Biomed Sci 9(6):656–664. https://doi.org/10.1159/000067281

    Article  CAS  PubMed  Google Scholar 

  12. Mali PY, Panchal SS (2013) A review on phytopharmacological potentials of Euphorbia thymifolia L. Anc Sci Life 32(3):165–172. https://doi.org/10.4103/0257-7941.123001

    Article  PubMed  PubMed Central  Google Scholar 

  13. Nadkarni KM, Nadkarni AK (2007) Indian MateriaMedica, vol 1. Popular Prakashan, Bombay, p 529

    Google Scholar 

  14. Basma AA, Zakaria Z, Latha LY, Sasidharan S (2011) Antioxidant activity and phytochemical screening of the methanol extracts of Euphorbia hirta L. Asian Pac J Trop Med 4:386–390. https://doi.org/10.1016/S1995-7645(11)60109-0

    Article  CAS  PubMed  Google Scholar 

  15. Mustafa RA, Abdul HA, Mohamed S, Bakar FA (2010) Total phenolic compounds, flavonoids, and radical scavenging activity of 21 selected tropical plants. J Food Sci 75:28–35. https://doi.org/10.1111/j.1750-3841.2009.01401.x

    Article  CAS  Google Scholar 

  16. Wu Y, Qu W, Geng D, Liang JY, Luo YL (2012) Phenols and flavonoids from the aerial part of Euphorbia hirta. Chin J Nat Med 10(1):40–42. https://doi.org/10.1016/S1875-5364(12)60009-0

    Article  CAS  PubMed  Google Scholar 

  17. Kumar A, Intekhab J (2013) Phytochemical studies on Euphorbia hypericifolia. Natura Proda Medica 5(1):2–5

    Google Scholar 

  18. Mukherjee D, Kuma NS, Khatua T, Mukherjee PK (2010) Rapid validated HPTLC method for estimation of betulinic acid in Nelumbonucifera (Nymphaeaceae) rhizome extract. Phytochem Anal 21:556–560. https://doi.org/10.1002/pca.1232

    Article  CAS  PubMed  Google Scholar 

  19. Ciesla L, Hajnos M, Staszek D, Wojtal L, Kowalska T, Hajnos MWV (2010) Validated Binary high-performance thin-layer chromatographic fingerprints of polyphenolics for distinguishing different Salvia species. J Chromatogr Sci 48:421–427. https://doi.org/10.1093/chromsci/48.6.421

    Article  CAS  PubMed  Google Scholar 

  20. Ghosh P, Katiyar A (2012) Densitometric HPTLC analysis of juglone, quercetin, myricetin, rutin, caffeic acid, and gallic acid in Juglans regia L. J Planar Chromatogr 5:420–425. https://doi.org/10.1556/jpc.25.2012.5.6

    Article  Google Scholar 

  21. Patel AA, Amin AA, Patwari AH, Shah MB (2017) Validated high performance thin layer chromatography method for simultaneous determination of quercetin and gallic acid in Leeaindica. Rev Bras Farmacogn 27(1):50–53. https://doi.org/10.1016/j.bjp.2016.05.017

    Article  CAS  Google Scholar 

  22. Hussain J, Bassal M, Sarhan H, Mouhammad IHA (2017) Qualitative and quantitative comparison of rutin, quercetin and gallic acid concentrations in Syrian Capparisspinosa L Leaves. J Pharmacogn Phytochem 6(4):407–415

    CAS  Google Scholar 

  23. NairJ Ahamad J, Khan W, Anjum V, Mathur R (2017) Development and validation of high-performance thin-layer chromatography method for simultaneous determination of polyphenolic compounds in medicinal plants. Pharmacogn Res 9(Suppl 1):S67–S73. https://doi.org/10.4103/pr.pr_122_16

    Article  CAS  Google Scholar 

  24. International Conference on Harmonisation, Guideline Q2 (R1) (2005) Validation of analytical procedures: text and methodology. ICH, Geneva. http://www.ich.org/LOB/media/MEDIA417.pdf

  25. Dutta S, Ray S (2020) Comparative assessment of total phenolic content and in vitro antioxidant activities of bark and leaf methanolic extracts of Manilkarahexandra (Roxb.) Dubard. J King Saud Univ Sci 32(1):643–647. https://doi.org/10.1016/j.jksus.2018.09.015

    Article  Google Scholar 

  26. Blainski A, CristinyGL Carlos JPM (2013) Application and analysis of the Folin–Ciocalteu method for the determination of the total phenolic content from Limonium brasiliense L. Molecules 18:6852–6865. https://doi.org/10.3390/molecules18066852

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Funding

No funding was received for conducting this study.

Author information

Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Saurashtra University, Rajkot, Gujarat, India

    Jigna M. Vadalia & Jalpa Sanandia

  2. Gujarat Technological University, Ahmedabad, Gujarat, India

    Navin Sheth

Authors
  1. Jigna M. Vadalia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jalpa Sanandia
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Navin Sheth
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jigna M. Vadalia.

Ethics declarations

Conflict of interest

The authors have no conflict of interest to declare that are relevant to the content of this article.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 180 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Vadalia, J.M., Sanandia, J. & Sheth, N. Comparative quantitative phytochemical and HPTLC analysis of two Euphorbiaceae family plants under the name Dugdhika. JPC-J Planar Chromat 33, 473–479 (2020). https://doi.org/10.1007/s00764-020-00059-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00764-020-00059-4

Keywords

Search

Navigation