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Rapid Deconstruction of Cotton, Coir, Areca, and Banana Fibers Recalcitrant Structure Using a Bacterial Consortium with Enhanced Saccharification

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Abstract

This study aims to establish a bacterial consortium to deconstruct the coir, areca, banana, and cotton fibers recalcitrant structure. Hence, lignocellulase secreting bacterial strains were isolated from cow rumen, dung garbage, and vermicompost samples. After selecting desired strains, a mixed-batch bacterial consortium (MBBC) was made by mixing of cellulase batch (Bacillus sp. HSTU-2, Bacillus sp. HSTU-3, Citrobacter sp. HSTU-AAJ4), pectinase batch (Acinetobacter sp. HSTU-6, Bacillus sp. HSTU-7, Enterobacter sp. HSTU-AAH8), and amylase batch (Bacillus sp. HSTU-9, and Bacillus sp. HSTU-10) strains. Separately, each batch and MBBC strains were largely grown in the culture medium enriched with coir, areca, banana, and cotton fibers; as a consequence, the hardy lignocellulosic fibers were degraded. FTIR study indicated that the peak intensities for lignin was diminished, but sharpened for cellulose in the MBBC than that of the single batch pretreatment. The MBBC pretreatment could remove 64–73% lignin from banana, areca, and coir fibers, resulting in increasing cellulose amounts. The crystallinity index was observed 31.5%, 21.71%, 35%, and 29.26% for the untreated and 13.69%, 18.27%, 17.72%, and 25.20% for the MBBC pretreated cotton, areca, coir, and banana fibers, respectively. The MBBC pretreated banana, areca, coir, and cotton fibers could generate reducing sugars that scaled up to 5.3-, 3.9-, 3.68-, and 2.68-fold greater than the untreated samples. Hence, it is radically feasible to produce bioethanol precursors from the 4-days long MBBC pretreated banana, areca, and coir lignocelluloses. This research revealed the shortest lignocellulose pretreatment duration with a bacterial consortium holding minimal community members.

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Acknowledgements

The research was supported by The World Academy of Sciences (TWAS), Trieste, Italy, and as a research grant (Individual) winner. Dr. Md. Azizul Haque would like to express gratitude to TWAS (Research Grants: 17-475 RG/BIO/AS_I, January, 2018 -June, 2020). The authors would like to acknowledge the Ministry of Science and Technology, Government of Bangladesh (MOST-2017) for the assistance.

Funding

The research was supported by The World Academy of Sciences (TWAS), Trieste, Italy, and as a research grant (Individual) winner Dr. Md. Azizul Haque would like to express gratitude to TWAS (Research Grants: 17–475 RG/BIO/AS_I, January, 2018 -June, 2020). The authors would like to acknowledge the Ministry of Science and Technology, Government of Bangladesh (MOST-2017) for the initial funding assistance.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, 5200, Bangladesh

    Md. Azizul Haque, Md. Ashikujjaman Ashik, Shefali Aktar, Most. Sarmin Akter, Abubakar Halilu, Md. Abdullah-Al-Mamun, Mst. Nur-E-Nazmun Nahar & Shukla Rani Das

  2. School of Engineering, Royal Melbourne Institute of Technology, Victoria, Australia

    Shefali Aktar

  3. Department of Pharmaceutical Sciences, Usmanu Danfodiyo University, Sokoto State, Nigeria

    Abubakar Halilu

  4. Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, 5200, Bangladesh

    Md. Atiqul Haque

  5. Key Lab of Animal Epidemiology and Zoonoses of Ministry of Agriculture, China Agricultural University, Beijing, 100193, China

    Md. Atiqul Haque

  6. Department of Fisheries Technology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, 5200, Bangladesh

    Md. Reazul Islam

  7. School of Life Sciences, University of Nottingham, Nottingham, UK

    Shukla Rani Das

  8. Division of Molecular Biotechnology, National Institute of Biotechnology, Savar, Dhaka, Bangladesh

    Keshob Chandra Das & Irfan Ahmed

  9. Institute of Radiation and Polymer Technology, Bangladesh Atomic Energy Research Establishment, Savar, Dhaka, Bangladesh

    Md. Serajum Manir

  10. Wazed Miah Science Research Centre, Jahangirnagar University, Savar, Dhaka, Bangladesh

    Md. Khairul Islam

  11. Department of Mechanical Engineering, Michigan State University, East Lansing, MI, USA

    Muhammad Rubayat Bin Shahadat

  12. Department of Mechanical Engineering, Hajee Mohammad Danesh Science and Technology University, Dinajpur, 5200, Bangladesh

    Muhammad Rubayat Bin Shahadat

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  1. Md. Azizul Haque
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Contributions

Dr. Md. Azizul Haque have conceived, designed, supervised the experiments, and analyzed the data as well as written the manuscript. Md. Ashikujjaman Ashik, Most. Sarmin Akter, Abubakar Halilu performed experiments and written their own thesis for graduation. Shefali Aktar, Dr. Mst. Nur-E- Nazmun Nahar, and Shukla Rani Das has superivesed and cosupervised the graduate students and comments on the data during experiments. Dr. Md. Atiqul Haque and Shefali Aktar was involved in the fund hunting with Dr. Md. Azizul Haque. Dr. Md. Reazul Islam, Dr. Md. Atiqul Haque, and Muhammad Rubayat Bin Shahadat carefully proofread the manuscript with and Dr. Md. Azizul Haque. Md. Abdullah-Al-Mamun, Keshob Chandra Das, Irfan Ahmed, Md. Ashikujjaman, and Dr. Md. Azizul Haque was involved in DNA sequencing and Analysis. Dr. Md. Azizul Haque, Md. Serajum Manir, and Md. Khairul Islam performed the FT-IR and X-ray Diffraction analyses of the samples.

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Correspondence to Md. Azizul Haque.

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Haque, M.A., Ashik, M.A., Aktar, S. et al. Rapid Deconstruction of Cotton, Coir, Areca, and Banana Fibers Recalcitrant Structure Using a Bacterial Consortium with Enhanced Saccharification. Waste Biomass Valor 12, 4001–4018 (2021). https://doi.org/10.1007/s12649-020-01294-w

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