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Aqueous extract of Phragmites communis rhizomes attenuates phototoxicity in skin cells

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A Correction to this article was published on 14 December 2020

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Abstract

Background

Excessive sunlight exposure leads to photodamaged skin, resulting in wrinkles, roughness, relaxation, and pigmentation. We examined photoprotective effects of aqueous extracts of dried Phragmites communis rhizome (PCWE) on ultraviolet B radiation (UVB)-mediated photodamage in skin cells.

Methods

Human dermal fibroblasts (HDFs), melanocytes (B16F10 cells), and keratinocytes (HaCaT cells) were treated with PCWE (25–200 μg/mL), with or without UVB (30 mJ/cm2). Cell viability, cell senescence, and mRNA levels of genes involved in skin homeostasis were assessed. Anti-melanogenic effects of PCWE on B16F10 cells were evaluated. Cyclooxygenase-2 (COX-2) mRNA levels and β-hexosaminidase release were evaluated in macrophage RAW264.7 and basophilic leukemia RBL-2H3 cells, respectively.

Results

No significant cytotoxicity was observed in tested cells up to 200 μg/mL PCWE. In HDFs and HaCaT cells, PCWE pretreatment afforded significant, concentration-dependent photoprotection. PCWE downregulated baseline matrix metalloprotease-1 expression and elastase activity in HDFs; in HaCaT cells, telomerase reverse transcriptase and hyaluronan synthase-2 expressions were upregulated. PCWE suppressed α-melanocyte-stimulating hormone-mediated increase in melanin production and tyrosinase activity. PCWE suppressed COX-2 induction (in RAW264.7) and β-hexosaminidase release (in RBL-2H3).

Conclusion

PCWE exhibits good potential to attenuate photodamage in skin cells.

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Change history

  • 14 December 2020

    Unfortunately the second affiliation of the author Eun-Hwa Sohn has been omitted.

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Acknowledgements

This research was conducted as part of the project entitled “Development of multi-functional cosmetic materials derived from halophytes” funded by the Ministry of Oceans and Fisheries, Korea.

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Author notes

  1. Sung Hyeok Kim, Chang Woo Ha and Hyosun Lim contributed equally to this work.

Authors and Affiliations

  1. College of Health Sciences, Kangwon National University, Samcheok, 25949, Republic of Korea

    Sung Hyeok Kim, Chang Woo Ha, Sohee Jang, Seung Namkoong, Sungsil Hong & Eun-Hwa Sohn

  2. Korea Research Institute BioScience Co., Ltd, Yeoju-si, 12668, Republic of Korea

    Hyosun Lim & Youn Kyu Kim

  3. Department of Convergence Biomedical Science, Cardiovascular and Metabolic Disease Center, College of Medicine, Inje University, Busan, 47392, Republic of Korea

    Sung Ryul Lee

  4. Graduate School of Analytical Science and Technology (GRAST), Chungnam National University, Daejeon, 34134, Republic of Korea

    Hyosun Lim & Jae-Young Kim

Authors
  1. Sung Hyeok Kim
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  2. Chang Woo Ha
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  3. Hyosun Lim
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Contributions

SHK, CWH, H–SL, J-YK, SRL, and E-HS designed the research study. SHK, CWH, H-SL, J-YK, S-HJ, SN, S-SH, YKK performed the experiments and analyzed data. SHK, CWH, H-SL, SRL, and E-HS interpreted data and wrote the manuscript.

Corresponding authors

Correspondence to Sung Ryul Lee or Eun-Hwa Sohn.

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Conflict of interest

Sung Hyeok Kim, Chang Woo Ha, Hyosun Lim, Sohee Jang, Seung Namkoong, Sungsil Hong, Youn Kyu Kim, Jae-Young Kim, Sung Ryul Lee, and Eun-Hwa Sohn declare that they have no conflict of interest.

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The article does not contain any studies with human and animal and this study was performed following institutional and national guidelines.

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Kim, S.H., Ha, C.W., Lim, H. et al. Aqueous extract of Phragmites communis rhizomes attenuates phototoxicity in skin cells. Mol. Cell. Toxicol. 17, 29–40 (2021). https://doi.org/10.1007/s13273-020-00106-5

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