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Evaluation of the combined application of Purpureocillium lilacinum PLSAU-1 and Glomus sp. against Meloidogyne incognita: implications for arsenic phytotoxicity on eggplant

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Abstract

Glasshouse pot experiments were conducted to evaluate the efficacy of Purpureocillium lilacinum PLSAU-1 (Pl) either alone or in combination with Glomus sp. (G) to control southern root-knot nematode, Meloidogyne incognita (Mi) in Solanum melongena in arsenic (AS)-contaminated soil. Root fragments of maize seedlings were applied to 5 g roots containing approximately 200 G colonized root fragments/100 g potting soil and G colonized 100 g rhizosphere soil of maize containing 30 chlamydospores/g soil and thoroughly mixed with sterilized potting soil for the G treatment. Pl was applied to the potting soil at 5 × 106colony-forming units (CFU/g soil). AS solution was added to the potting soil at 50 mg kg−1. Sixteen treatments comprising single and combined applications of Pl, G, AS, and Mi with five replications were applied and pots were randomly arranged in the glasshouse. Single nematode-free eggplant seedlings (30-days old) were transplanted. Eggs of Mi were inoculated at 10000 eggs/seedling pot. Data were recorded on root gall index, plant growth parameters, N, P, K and S uptake, and AS uptake 2 months after transplantation. The combined application of Pl and G enhanced plant growth, leaf area, chlorophyll content, nutrients uptake and reduced AS toxicity. Gall index and AS uptake were reduced by 84.50% and 51.72%, respectively with the combined application of Pl and G. We conclude that Pl and G can be integrated as biological management tools against Mi in AS polluted vegetable growing areas.

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Acknowledgements

We thank the anonymous reviewers for their kind review of this article. This research work was supported by the TWAS (The World Academy of Sciences for the advancement of Science in developing countries) through a grant to F. M. Aminuzzaman [Research Grant No.:13-246 RG/BIO/AS_/UNESCO FR: 3240277693].

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  1. Department of Plant Pathology, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    H. Khalid, F. M. Aminuzzaman, K. Amit, A. A. Faria, A. I. Mitu, M. S. M. Chowdhury & J. Shammi

  2. Department of Soil Science, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    M. A. Khan

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Correspondence to F. M. Aminuzzaman.

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Khalid, H., Aminuzzaman, F.M., Amit, K. et al. Evaluation of the combined application of Purpureocillium lilacinum PLSAU-1 and Glomus sp. against Meloidogyne incognita: implications for arsenic phytotoxicity on eggplant. Eur J Plant Pathol 159, 139–152 (2021). https://doi.org/10.1007/s10658-020-02150-2

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