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Muricauda brasiliensis sp. nov., isolated from a mat-forming cyanobacterial culture

  • Environmental Microbiology - Research Paper
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Abstract

Strain K001 was isolated from a cyanobacterial culture derived from Abrolhos, a reef bank microbial mat (South Atlantic Ocean—Brazil). Cells of K001 are Gram stain–negative, catalase and oxidase-positive, non-motile, rod-shaped, and with or without appendages. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain K001 belongs to the genus Muricauda. The highest strain K001 16S rRNA gene identity, ANI, and dDDH, respectively, are with M. aquimarina (98.90%, 79.23, 21.60%), M. ruestringensis (98.20%, 80.82, 23.40%), and M. lutimaris (97.86%, 79.23, 22.70%). The strain grows at 15–37 °C and between 0.5 and 10% NaCl. The major fatty acids of strain K001 are iso-C15:0, iso-C15:1 G, iso-C17:0 3-OH, and summed feature 3 (C16:1 ω6c and/or C16:1 ω7c). The polar lipids are represented by phosphatidylethanolamine, three unidentified aminolipids, and three unidentified polar lipids. The major respiratory quinone is MK-6. The G+C content of the DNA of strain K001 is 41.62 mol%. Based on polyphasic analysis of strain K001, it was identified as a novel representative of the genus Muricauda and was named Muricauda brasiliensis sp. nov. The type strain is K001 (=CBMAI 2315T = CBAS 752T).

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Abbreviations

MA:

marine agar DIFCO 2216

MB:

marine broth DIFCO 2216

MALDI-TOF:

matrix-assisted laser desorption/ionization

MS:

mass spectrogram

dDDH:

digital DNA–DNA hybridization

ANI:

average nucleotide identity

TYGS:

Type Strain Genome Server

MIDI:

Sherlock Microbial Identification System

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Acknowledgments

We thank the Laboratory of Environmental Sanitation, Department of Civil and Environmental Engineering, University of Brasilia (Brazil), for the infrastructure to maintain the cultures and to perform some analyses. We thank the DNA Services Facility in Research Resources Center, University of Illinois at Chicago, Chicago (USA) for the sequencing service. We are grateful to the Microscopy and Microanalysis Laboratory of the University of Brasilia (Brazil), for their collaboration in carrying out the scanning electron microscopy analysis. We thank the Laboratory of Toxinology (Department of Physiological Sciences, Biological Sciences Institute, University of Brasilia, Brazil), the Laboratories of Graduate Program in Genomics Science and Biotechnology (Catholic University of Brasilia, Brasilia, Brazil), Laboratory of Mass Spectrometry (EMBRAPA Genetic Resources and Biotechnology, Brasilia, Brazil), and the Laboratories of the Department of Microbiology (Federal University of Viçosa, Viçosa, Brazil) for the infrastructure to perform part of the experiments of this work. We thank Dr. Manuela da Silva for the K001 microbial collections deposition efforts.

Funding

This research was supported by a grant from BioTecMar (no. 408339/2013-6) of CNPq (Ministry of Science, Technology, Innovations and Communications—Brazil), the Foundation for Research Support of the Federal District (FAP-DF), and Coordination for the Improvement of Higher Education Personnel (CAPES).

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Authors and Affiliations

  1. Department of Cellular Biology, Biological Sciences Institute, University of Brasília, Brasília, DF, Brazil

    Carla Simone Vizzotto, Julianna Peixoto, Fabyano Alvares C. Lopes, Otávio Henrique B. Pinto & Ricardo Henrique Krüger

  2. Department of Civil and Environmental Engineering, University of Brasília, Brasília, DF, Brazil

    Carla Simone Vizzotto

  3. Genome Research Core, Research Resources Center, University of Illinois at Chicago, Chicago, IL, USA

    Stefan Joshua Green

  4. Laboratory of Microbiology, Federal University of Tocantins, Porto Nacional, TO, Brazil

    Fabyano Alvares C. Lopes

  5. Graduate Program in Genomics Science and Biotechnology, Catholic University of Brasilia, Brasilia, DF, Brazil

    Marcelo Henrique S. Ramada

  6. Graduate Program in Gerontology, Catholic University of Brasilia, Brasilia, DF, Brazil

    Marcelo Henrique S. Ramada

  7. Toxinology Laboratory, Depto. Physiological Sciences, Institute of Biology, University of Brasilia, Brasilia, 70910-900, DF, Brazil

    Osmindo R. Pires Júnior

  8. Laboratories of the Department of Microbiology, Federal University of Viçosa, Viçosa, MG, Brazil

    Marcos Rogério Tótola

  9. Laboratory of Microbiology, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    Fabiano Lopes Thompson

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  1. Carla Simone Vizzotto
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Correspondence to Ricardo Henrique Krüger.

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Accession numbers: The GenBank/EMBL/DDBJ accession numbers of K001T strain for the 16S rRNA gene sequence is MN996941, and the draft genome sequence is QBTW00000000.

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Vizzotto, C.S., Peixoto, J., Green, S.J. et al. Muricauda brasiliensis sp. nov., isolated from a mat-forming cyanobacterial culture. Braz J Microbiol 52, 325–333 (2021). https://doi.org/10.1007/s42770-020-00400-3

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