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Immunological and bacteriological shifts associated with a flagellin-hyperproducing Salmonella Enteritidis mutant in chickens

  • Veterinary Microbiology - Research Paper
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Abstract

Salmonella Enteritidis causes infections in humans and animals which are often associated with extensive gut colonization and bacterial shedding in faeces. The natural presence of flagella in Salmonella enterica has been shown to be enough to induce pro-inflammatory responses in the gut, resulting in recruitment of polymorphonuclear cells, gut inflammation and, consequently, reducing the severity of systemic infection in chickens. On the other hand, the absence of flagellin in some Salmonella strains favours systemic infection as a result of the poor intestinal inflammatory responses elicited. The hypothesis that higher production of flagellin by certain Salmonella enterica strains could lead to an even more immunogenic and less pathogenic strain for chickens was here investigated. In the present study, a Salmonella Enteritidis mutant strain harbouring deletions in clpP and fliD genes (SE ΔclpPfliD), which lead to overexpression of flagellin, was generated, and its immunogenicity and pathogenicity were comparatively assessed to the wild type in chickens. Our results showed that SE ΔclpPfliD elicited more intense immune responses in the gut during early stages of infection than the wild type did, and that this correlated with earlier intestinal and systemic clearance of the bacterium.

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Funding

This work was supported by São Paulo Research Foundation (FAPESP) (grant numbers: 2018/04883-8 (F. O. Barbosa); 2016/10369-0 (A. Berchieri Jr)), Coordination of Improvement of Higher Education Personnel (CAPES), and National Council of Technological and Scientific Development (CNPq).

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Authors and Affiliations

  1. Department of Veterinary Pathology from the School of Agriculture and Veterinarian Sciences (FCAV), Jaboticabal, State University of São Paulo, Jaboticabal, Brazil

    Fernanda de Oliveira Barbosa, Lucas Bocchini Rodrigues Alves, Valdinete Pereira Benevides, Andrei Itajahy Secundo de Souza, Marcela da Silva Rubio, Adriana Maria de Almeida, Mauro Mesquita Saraiva & Angelo Berchieri Junior

  2. Department of Preventive Veterinary Medicine, Veterinary School, Federal University of Minas Gerais (UFMG), Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, 31270-901, Brazil

    Oliveiro Caetano de Freitas Neto

  3. Department of Animal Science, Center for Agricultural Sciences, Federal University of Paraiba (CCA/UFPB), Areia, PB, Brazil

    Celso José Bruno de Oliveira

  4. Department of Veterinary and Animal Sciences, University of Copenhagen, Stigbøjlen 4, 1870, Frederiksberg C, Denmark

    John Elmerdahl Olsen

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  1. Fernanda de Oliveira Barbosa
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  2. Oliveiro Caetano de Freitas Neto
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  9. Celso José Bruno de Oliveira
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Correspondence to Oliveiro Caetano de Freitas Neto.

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de Oliveira Barbosa, F., de Freitas Neto, O.C., Rodrigues Alves, L.B. et al. Immunological and bacteriological shifts associated with a flagellin-hyperproducing Salmonella Enteritidis mutant in chickens. Braz J Microbiol 52, 419–429 (2021). https://doi.org/10.1007/s42770-020-00399-7

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