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Genetic diversity and structure analysis of Vigna unguiculata L. (Walp.) landraces from southeastern Mexico using ISSR markers

Published online by Cambridge University Press:  04 November 2020

L. F. C. dos Santos Open the ORCID record for L. F. C. dos Santos [Opens in a new window] ,
M. M. Ferrer ,
M. R. Ruenes-Morales ,
P. I. Montañez-Escalante ,
R. H. Andueza-Noh  and
J. Jiménez-Osornio
L. F. C. dos Santos
Affiliation:
Departamento de Manejo y Conservación de Recursos Naturales Tropicales, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Carretera Mérida-Xmatkuil Km. 15.5, 97315Mérida, Yucatán, México
M. M. Ferrer
Affiliation:
Departamento de Manejo y Conservación de Recursos Naturales Tropicales, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Carretera Mérida-Xmatkuil Km. 15.5, 97315Mérida, Yucatán, México
M. R. Ruenes-Morales
Affiliation:
Departamento de Manejo y Conservación de Recursos Naturales Tropicales, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Carretera Mérida-Xmatkuil Km. 15.5, 97315Mérida, Yucatán, México
P. I. Montañez-Escalante
Affiliation:
Departamento de Manejo y Conservación de Recursos Naturales Tropicales, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Carretera Mérida-Xmatkuil Km. 15.5, 97315Mérida, Yucatán, México
R. H. Andueza-Noh
Affiliation:
Conacyt-División de Estudios de Posgrado e Investigación, Instituto Tecnológico de Conkal, Avenida Tecnológico S/N CP 97345Conkal, Yucatán, México
J. Jiménez-Osornio*
Affiliation:
Departamento de Manejo y Conservación de Recursos Naturales Tropicales, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Carretera Mérida-Xmatkuil Km. 15.5, 97315Mérida, Yucatán, México
*
*Corresponding author. E-mail: josornio@correo.uady.mx
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Abstract

Cowpea (Vigna unguiculata L. Walp.) is an important grain legume in tropical and subtropical regions. It requires low resource inputs and has a high nutritional value. Therefore, cowpea can play an important role in the development of agriculture. In southern Mexico, Mayan farmers have conserved and developed cowpea landraces for centuries. Nevertheless, information on their genetic diversity, conservation status and potential use is minimal. To generate information toward sustainable use, management and conservation of this species, we evaluated the genetic diversity and structure of 20 cowpea landraces from southeast Mexico using 10 inter-simple sequence repeat (ISSR) molecular markers. These ISSR markers generated 68 loci with a 67.7% polymorphism rate and average polymorphic information content of 0.36. The results of Bayesian assignation and the UPGMA analysis suggest the formation of two main groups defined by their genetic origin in southeast Mexico. High levels of genetic structure were found with a moderate level of genetic diversity distributed mainly between landraces. Low levels of intra-landrace variability were observed. Two landraces (P5 and P12) from Calakmul resulted in the high levels of genetic diversity. The selected markers were efficient at assessing genetic variability among Mexican cowpea landraces, providing valuable information that can be used in local conservation and participatory breeding programmes.

Keywords

Cowpeagenetic resourceslandrace conservationmolecular markerspolymorphism
Type
Research Article
Information
Plant Genetic Resources , Volume 18 , Issue 4 , August 2020 , pp. 201 - 210
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press on behalf of NIAB

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