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A potential endogenous gibberellin-mediated signaling cascade regulated floral transition in Magnolia × soulangeana ‘Changchun’

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Abstract

The floral transition is a critical developmental switch in plants, and has profound effects on the flower production and yield. Magnolia × soulangeana ‘Changchun’ is known as a woody ornamental plant, which can bloom in spring and summer, respectively. In this study, anatomical observation, physiological measurement, transcriptome, and small RNA sequencing were performed to investigate potential endogenous regulatory mechanisms underlying floral transition in ‘Changchun’. Transition of the shoot apical meristem from vegetative to reproductive growth occurred between late April and early May. During this specific developmental process, a total of 161,645 unigenes were identified, of which 73,257 were significantly differentially expressed, while a number of these two categories of miRNAs were 299 and 148, respectively. Further analysis of differentially expressed genes (DEGs) revealed that gibberellin signaling could regulate floral transition in ‘Changchun’ in a DELLA-dependent manner. In addition, prediction and analysis of miRNA targeted genes suggested that another potential molecular regulatory module was mediated by the miR172 family and other several novel miRNAs (Ms-novel_miR139, Ms-novel_miR229, and Ms-novel_miR232), with the participation of up- or down-regulating genes, including MsSVP, MsAP2, MsTOE3, MsAP1, MsGATA6, MsE2FA, and MsMDS6. Through the integrated analysis of mRNA and miRNA, our research results will facilitate the understanding of the potential molecular mechanism underlying floral transition in ‘Changchun’, and also provide basic experimental data for the plant germplasm resources innovation in Magnolia.

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Data availability

The raw sequence data from this study have been deposited at NCBI Sequence Read Archive (Accession Number PRJNA663115, PRJNA663196).

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Acknowledgements

We thank Dr. Qiang Wei (Bamboo Research Institute, Nanjing Forestry University) for his useful advice and also help with molecular experiments.

Funding

This work was supported by grants from the Postgraduate Research & Practice Innovation Program of Jiangsu Province (Grant Number SJKY19_0917), A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and the Doctorate Fellowship Foundation of Nanjing Forestry University.

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  1. Liyong Sun and Zheng Jiang contributed equally to this work.

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  1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China

    Liyong Sun, Zheng Jiang, Ye Ju, Xuan Zou, Xiaoxia Wan, Yao Chen & Zengfang Yin

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Contributions

Z.-F.Y. designed and supervised the project, reviewed drafts of the paper; L.-Y.S. conceived the conception, performed part of experiments, analyzed the data, and wrote the original draft manuscript; Z.J. collected the samples, analyzed the data, performed part of experiments, and checked and revised the manuscript; Y.J. performed the qRT-PCR experiment; X.Z. and X.-X.W. participated in analyzing the data and drew some figures and tables; Y.C. performed the phenologic observation.

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Correspondence to Zengfang Yin.

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Sun, L., Jiang, Z., Ju, Y. et al. A potential endogenous gibberellin-mediated signaling cascade regulated floral transition in Magnolia × soulangeana ‘Changchun’. Mol Genet Genomics 296, 207–222 (2021). https://doi.org/10.1007/s00438-020-01740-3

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