Abstract
Transcription of defense genes in grapevines function by combining the transcription factors to overcome stresses caused by unfavorable environmental conditions. Heat shock transcriptional factors (HSFs) have the most important role in transcription of genes that respond to high temperatures. The present study identifies the structure and motif location of 19 grape HSF genes in Vitis flexuosa, and investigates their expression patterns in grapevines exposed to high temperatures. Examination of the V. flexuosa transcript database identified 19 VfHSFs, which include VHSFA, VHSFB, and VHSFC. Analysis of the amino acid sequences of VHSF genes revealed 10 motifs. Vines of ‘Campbell Early’, ‘Kyoho’ grapes, and V. flexuosa were exposed to 25 °C, 30 °C, 35 °C, 40 °C and 45 °C in incubation chambers, for varying time points (0, 3, 6, 12 and 24 h). Homologous genes (VfHSFs) of V. flexuosa were predicted by searching the HSF genes in the V. flexuosa transcripts database. Prediction of the protein structure demonstrated that all 19 VfHSF genes of V. flexuosa had an HSF domain. In order to validate the difference in gene expression among cultivars, the relative expression levels were measured using real-time PCR. We found 10 HSF genes (HSF2, HSF3, HSF4, HSF5, HSF7, HSF10, HSF14, HSF16, HSF17, and HSF18) showed differential expression patterns in response to high temperature in ‘Campbell Early’, ‘Kyoho’, and V. flexuosa grapevines. We consider that these results can be useful information to select genetic resources for the future development of heat tolerant grapes, and to study the reaction of grapevines to high temperatures.
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Data availability
Information of nucleotide sequencing of Vitis flexuosa heat shock transcription factor genes (VfHSFs) were submitted to National Agricultural Biotechnology Information Center, RDA, Korea (accession numbers are NS-2689 to NS-2707-000001). The datasets generated during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Export Promotion Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA, 617070-5).
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JL performed the overall experiment and data analysis. SK and SA performed experiment to confirm the results and wrote manuscript together. HY designed and managed whole experiments and finalized the manuscript.
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Lee, J.H., Kim, S.A., Ahn, S.Y. et al. Heat shock transcriptional factor genes (VfHSFs) of Vitis flexuosa respond differentially to high temperature in grapevines. Hortic. Environ. Biotechnol. 62, 87–97 (2021). https://doi.org/10.1007/s13580-020-00296-w
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DOI: https://doi.org/10.1007/s13580-020-00296-w