Abstract
Ethylene response factors (ERFs) widely exist in plants and have been reported to be an important regulator of plant abiotic stress. Celery, a common economic vegetable of Apiaceae, contains lots of ERF transcription factors (TFs) with various functions. AP2/ERF TFs play positive or negative roles in plant growth and stress response. Here, AgERF8, a gene encoding EAR-type AP2/ERF TF, was identified. The AgERF8 mRNA accumulated in response to both abscisic acid (ABA) signaling and salt treatment. AgERF8 was proving to be a nucleus-located protein and could bind to GCC-box. The overexpression of AgERF8 in Arabidopsis repressed the transcription of downstream genes, AtBGL and AtBCH. Arabidopsis overexpressing AgERF8 gene showed inhibited root growth under ABA and NaCl treatments. AgERF8 transgenic lines showed low tolerance to ABA and salt stress than wild-type plants. Low increment in SOD and POD activities, increased accumulation of MDA, and significantly decreased plant fresh weights and chlorophyll levels were detected in AgERF8 hosting lines after treated with ABA and NaCl. Furthermore, the overexpression of AgERF8 also inhibited the levels of ascorbic acid and antioxidant-related genes (AtCAT1, AtSOD1, AtPOD, AtSOS1, AtAPX1, and AtP5CS1) expression in transgenic Arabidopsis. This finding indicated that AgERF8 negatively affected the resistance of transgenic Arabidopsis to ABA and salt stress through regulating downstream genes expression and relevant physiological changes. It will provide a potential sight to further understand the functions of ERF TFs in celery.
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The research was supported by Jiangsu Agricultural Science and Technology Innovation Fund (CX(18)2007), New Century Excellent Talents in University (NCET-11-0670), National Natural Science Foundation of China (31272175), Priority Academic Program Development of Jiangsu Higher Education Institutions Project (PAPD).
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ASX and JXL initiated and designed the research; JXL, BW, AQD, MYL, DS, and YL performed the experiments; JXL, BW, and ZSX analyzed the data; ASX contributed to reagents/materials/analysis tools; JXL wrote the paper; and ASX and KF revised the paper. All authors read and approved the final manuscript.
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Liu, JX., Wu, B., Feng, K. et al. A celery transcriptional repressor AgERF8 negatively modulates abscisic acid and salt tolerance. Mol Genet Genomics 296, 179–192 (2021). https://doi.org/10.1007/s00438-020-01738-x
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DOI: https://doi.org/10.1007/s00438-020-01738-x