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Circ0001320 inhibits lung cancer cell growth and invasion by regulating TNFAIP1 and TPM1 expression through sponging miR-558

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Abstract

Lung cancer is the most affected malignant tumor in the world, and its specific pathogenesis is still unclear. It has been confirmed that circ0001320 is down-regulated in lung cancer, but its mechanism has not been reported. Further study found that circ0001320 was down-regulated in lung cancer cells, localized in the cytoplasm, and had multiple miR-558 binding sites. Dual-luciferase reporter gene assay, RNA-pull-down, and immunoprecipitation experiments all confirmed that circ0001320 directly bound to miR-558, and then inhibit the expression of miR-558. MiR-558 was up-regulated in lung cancer cells, and bound the downstream target genes TNFAIP1 and TPM1 to inhibit their expression. Western blot showed that circ0001320 significantly up-regulated the protein levels of TNFAIP1 and TPM1, while miR-558 blocked this effect of circ0001320. Circ0001320, TNFAIP1, and TPM1 all inhibited the proliferation and invasion of lung cancer cells and promoted apoptosis, while miR-558 had the opposite effects. After transfection with circ0001320 overexpression vector, miR-558 up-regulation or down-regulation of TNFAIP1, or TPM1 expression significantly reversed the inhibition of cell growth and invasion by circ0001320. Similarly, the expression of TNFAIP1 or TPM1 was down-regulated, while miR-558 expression was inhibited, and the levels of cell proliferation, apoptosis, and invasion did not change significantly. Therefore, these fully show that circ0001320 inhibits the growth and invasion of lung cancer cells through miR-558/TNFAIP1 and TPM1 pathways, which may be closely related markers and therapeutic targets of lung cancer.

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References

  1. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2020. CA Cancer J Clin. 2020;70(1):7–30.

    Article  Google Scholar 

  2. Patop IL, Kadener S. circRNAs in Cancer. Curr Opin Genet Dev. 2018;48:121–7.

    Article  CAS  Google Scholar 

  3. Hang D, Zhou J, Qin N, et al. A novel plasma circular RNA circFARSA is a potential biomarker for non-small cell lung cancer. Cancer Med. 2018;7(6):2783–91.

    Article  CAS  Google Scholar 

  4. Xu J, Shu Y, Xu T, et al. Microarray expression profiling and bioinformatics analysis of circular RNA expression in lung squamous cell carcinoma. Am J Transl Res. 2018;10(3):771–83.

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Qu H, Zheng L, Pu J, et al. miRNA-558 promotes tumorigenesis and aggressiveness of neuroblastoma cells through activating the transcription of heparanase. Hum Mol Genet. 2015;24(9):2539–51.

    Article  CAS  Google Scholar 

  6. Xiao Y, Huang S, Qiu F, et al. Tumor necrosis factor α-induced protein 1 as a novel tumor suppressor through selective downregulation of CSNK2B blocks nuclear factor-κB activation in hepatocellular carcinoma. EBioMedicine. 2020;51:102603.

    Article  Google Scholar 

  7. Bharadwaj S, Prasad GL. Tropomyosin-1, a novel suppressor of cellular transformation is downregulated by promoter methylation in cancer cells. Cancer Lett. 2002;183(2):205–13.

    Article  CAS  Google Scholar 

  8. Yan Y, Zhang R, Zhang X, et al. RNA-Seq profiling of circular RNAs and potential function of hsa_circ_0002360 in human lung adenocarcinom. Am J Transl Res. 2019;11(1):160–75.

    PubMed  PubMed Central  Google Scholar 

  9. Di X, Jin X, Li R, et al. CircRNAs and lung cancer: biomarkers and master regulators. Life Sci. 2019;220:177–85.

    Article  CAS  Google Scholar 

  10. Gu X, Wang G, Shen H, Fei X. Hsa_circ_0033155: a potential novel biomarker for non-small cell lung cancer. Exp Ther Med. 2018;16(4):3220–6.

    PubMed  PubMed Central  Google Scholar 

  11. Huang MS, Liu JY, Xia XB, et al. Hsa_circ_0001946 inhibits lung cancer progression and mediates cisplatin sensitivity in non-small cell lung cancer via the nucleotide excision repair signaling pathway. Front Oncol. 2019;9:508.

    Article  Google Scholar 

  12. Qu H, Zheng L, Song H, et al. microRNA-558 facilitates the expression of hypoxia-inducible factor 2 alpha through binding to 5’-untranslated region in neuroblastoma. Oncotarget. 2016;7(26):40657–73.

    Article  Google Scholar 

  13. Zheng L, Jiao W, Song H, et al. miRNA-558 promotes gastric cancer progression through attenuating Smad4-mediated repression of heparanase expression. Cell Death Dis. 2016;7(9):e2382.

    Article  CAS  Google Scholar 

  14. Shohet JM, Ghosh R, Coarfa C, et al. A genome-wide search for promoters that respond to increased MYCN reveals both new oncogenic and tumor suppressor microRNAs associated with aggressive neuroblastoma. Cancer Res. 2011;71(11):3841–51.

    Article  CAS  Google Scholar 

  15. Zhang M, Gao C, Yang Y, et al. MiR-424 promotes non-small cell lung cancer progression and metastasis through regulating the tumor suppressor gene TNFAIP1. Cell Physiol Biochem. 2017;42(1):211–21.

    Article  CAS  Google Scholar 

  16. Cui R, Meng W, Sun HL, et al. MicroRNA-224 promotes tumor progression in nonsmall cell lung cancer. Proc Natl Acad Sci USA. 2015;112(31):E4288–97.

    Article  CAS  Google Scholar 

  17. Zhang CL, Wang C, Yan WJ, et al. Knockdown of TNFAIP1 inhibits growth and induces apoptosis in osteosarcoma cells through inhibition of the nuclear factor-κB pathway. Oncol Rep. 2014;32(3):1149–55.

    Article  CAS  Google Scholar 

  18. Zhu Y, Yao Z, Wu Z, et al. Role of tumor necrosis factor alpha-induced protein 1 in paclitaxel resistance. Oncogene. 2014;33(25):3246–55.

    Article  CAS  Google Scholar 

  19. Wang J, Tang C, Yang C, et al. Tropomyosin-1 functions as a tumor suppressor with respect to cell proliferation, angiogenesis and metastasis in renal cell carcinoma. J Cancer. 2019;10(10):2220–8.

    Article  CAS  Google Scholar 

  20. Tang C, Wang J, Wei Q, et al. Tropomyosin-1 promotes cancer cell apoptosis via the p53-mediated mitochondrial pathway in renal cell carcinoma. Oncol Lett. 2018;15(5):7060–8.

    PubMed  PubMed Central  Google Scholar 

  21. Du HQ, Wang Y, Jiang Y, et al. Silencing of the TPM1 gene induces radioresistance of glioma U251 cells. Oncol Rep. 2015;33(6):2807–14.

    Article  CAS  Google Scholar 

  22. Zhang C, Ma L, Niu Y, et al. Circular RNA in Lung Cancer Research: biogenesis, Functions, and Roles. Int J Biol Sci. 2020;16(5):803–14.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. The First Clinical College, Southern Medical University, No. 1023-1063, Shatai South Road, Baiyun District, Guangzhou, 510515, Guangdong, China

    Yong Mao & Jian-xing He

  2. Department of Thoracic Surgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, China

    Jia-xi He & Jian-xing He

  3. Department of Cardiothoracic Surgery, Ningbo First Hospital, Ningbo, 315010, China

    Yong Mao

  4. Ningbo University School of Medicine, Ningbo, 315211, China

    Mei Zhu

  5. Department of Respiratory Disease, Yinzhou Second Hospital, Ningbo, 315040, China

    Yong-quan Dong

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  1. Yong Mao
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Correspondence to Jian-xing He.

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This study was approved by the Ethics Committee of Yinzhou Second Hospital (reference number 2020011) and performed in accordance with Declaration of Helsinki.

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Mao, Y., He, Jx., Zhu, M. et al. Circ0001320 inhibits lung cancer cell growth and invasion by regulating TNFAIP1 and TPM1 expression through sponging miR-558. Human Cell 34, 468–477 (2021). https://doi.org/10.1007/s13577-020-00453-4

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  • DOI: https://doi.org/10.1007/s13577-020-00453-4

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